Planar optical waveguide-based biosensor for the quantitative detection of tumor markers

Mukundan, Harshini; J, Kubicek; Holt, Alicia; Shively, John E.; Martinez, Jennifer S.; Grace, Karen M.; Grace, W. Kevin; Swanson, Basil I.

doi:10.1016/j.snb.2009.01.073

Cited by 57 publications

(73 citation statements)

References 32 publications

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“…Lipid micelles for use in waveguide experiments were prepared as described previously [2,5,13]. Briefly, 2 mM DOPC and 1% cap-biotinyl (mol/mol) were combined in a glass tube and the chloroform evaporated under argon gas.…”

Section: Lipid Micelle Preparationmentioning

confidence: 99%

“…Incident light from a 635 nm diode laser (Coherent, Auburn, CA) (power of 440−443 µW) was coupled into the waveguide using its diffraction grading. The response signal was adjusted for maximum peak intensity using a spectrometer (USB2000, Ocean Optics,Winter Park, FL) interfaced with the instrument and an optical power meter (Thor Labs, Newton, NJ) [1,5,13].…”

Section: Lta Membrane Insertion Assaymentioning

confidence: 99%

“…Measurements are extremely rapid (3 sec), yield minimal non-specific and background signals, and can be adapted to the simultaneous detection of a wide variety of fluorescent labels. This biosensor has been previously validated for the detection of anthrax [3], influenza [4], breast cancer [5] and tuberculosis [6][7][8]. Here we have extended the utility of this platform to detect Gram-positive pathogens, which include the clinically relevant Staphylococcus spp.…”

Section: Introductionmentioning

confidence: 99%

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Membrane Insertion for Direct Detection of Lipoteichoic Acid

Kubicek-Sutherland

Hengartner

Mukundan

2017

Trans. Mat. Res. Soc. Japan

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The incorrect diagnosis of bacterial pathogens results in the misuse of antibiotics, a major contributor to the evolution of antibiotic resistance. The ability to rapidly identify a pathogen could facilitate more effective treatment strategies, reducing the unnecessary use of antibiotics. Current diagnostics of bacterial infections often require cell growth, and are therefore less capable of informing timely treatment options. Here, we describe the development of a rapid assay (<45 min) to identify Gram-positive bacteria directly in human serum without any growth or isolation required. We have developed a fluorescence-based immunoassay specifically targeting the Gram-positive bacterial biomarker lipoteichoic acid (LTA). LTA is an amphiphilic molecule with both hydrophobic and hydrophilic portions. Our approach, termed membrane insertion, exploits the amphiphilic biochemistry of the molecule by allowing it to passively insert itself into a lipid bilayer, thereby trapping it for subsequent probing with a fluorescently labeled α-LTA antibody. Using this assay, we have successfully detected less than 10 µg/ml of LTA directly in spiked human serum. We intend to combine this rapid assay with others currently in development into a diagnostic tool for point-of-care detection of bacterial pathogens.

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Section: Lipid Micelle Preparationmentioning

confidence: 99%

Section: Lta Membrane Insertion Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Membrane Insertion for Direct Detection of Lipoteichoic Acid

Kubicek-Sutherland

Hengartner

Mukundan

2017

Trans. Mat. Res. Soc. Japan

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“…Both systems utilize a thin waveguide film deposited on a substrate etched with grating couplers. Using this platform, biomarkers for breast cancer (Mukundan, Kubicek, et al, 2009), tuberculosis (multiplexed assays; Mukundan et al, 2012), and anthrax (multiplexed assays; Mukundan et al, 2010) have been detected at physiologically relevant concentrations in clinical fluids such as serum, nipple aspirate, and urine.…”

Section: Ngimat and Los Alamos National Laboratorymentioning

confidence: 99%

Total internal reflection fluorescence (TIRF) array biosensors for biothreat agents for food safety and food defense

Taitt¹,

North²

2015

High Throughput Screening for Food Safety Assessment

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“…[7][8][9][10][11] This biosensor relies on evanescent sensing on a planar waveguide surface to amplify the transduction signal of the fluorescently labeled antibodies to the target antigen. 7,12 We have also developed novel surface functionalization chemistries and target pull-down strategies to increase the sensitivity and specificity of our assays.…”

Section: Introductionmentioning

confidence: 99%

Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis

Sakamuri

Waters

et al. 2017

ANAL. SCI.

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Early and rapid detection of bovine tuberculosis (bTB) is critical to controlling the spread of this disease in cattle and other animals. In this study, we demonstrate the development of an immunoassay for the direct detection of the bovine bTB biomarker, lipomannan (LM) in serum using a waveguide-based optical biosensor. We apply an ultra-sensitive detection strategy developed by our team, termed lipoprotein capture, that exploits the pull-down of high-density lipoprotein (HDL) nanodiscs from cattle blood that allows for the recovery and detection of associated LM. We also profile the change in the expression of these TB biomarkers as a function of time from a small set of samples collected from studies of bovine TB-infected cattle. We demonstrate for the first time the direct detection of bovine LM in serum, and clearly show that the biomarker is expressed in detectable concentrations during the entire course of the infection.

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Planar optical waveguide-based biosensor for the quantitative detection of tumor markers

Cited by 57 publications

References 32 publications

Membrane Insertion for Direct Detection of Lipoteichoic Acid

Membrane Insertion for Direct Detection of Lipoteichoic Acid

Total internal reflection fluorescence (TIRF) array biosensors for biothreat agents for food safety and food defense

Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis

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