Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry

Pompach, Petr; Nováková, J.; Kavan, Daniel; Benada, Oldřich; Růžička, Viktor; Volný, Michael; Novák, Petr

doi:10.1373/clinchem.2015.244004

Cited by 19 publications

(13 citation statements)

References 37 publications

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“…For example, detection limit for leptin in serum is 160 ng/mL. Performance of this approach was proved by detection of haptoglobin in human serum from 116 patients [ 94 ]. This method allows to distinguish two isoforms of haptoglobin with affinity to the same antibody.…”

Section: Cytokine Detection Techniquesmentioning

confidence: 99%

Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research

Skalníková

Čížková

Cervenka

et al. 2017

IJMS

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Melanoma is a skin cancer with permanently increasing incidence and resistance to therapies in advanced stages. Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. Cytokines are key factors regulating immune response and intercellular communication in tumour microenvironment. Cytokines may be used in therapy of melanoma to modulate immune response. Cytokines also possess diagnostic and prognostic potential and cytokine production may reflect effects of immunotherapies. The purpose of this review is to give an overview of recent advances in proteomic techniques for the detection and quantification of cytokines in melanoma research. Approaches covered span from mass spectrometry to immunoassays for single molecule detection (ELISA, western blot), multiplex assays (chemiluminescent, bead-based (Luminex) and planar antibody arrays), ultrasensitive techniques (Singulex, Simoa, immuno-PCR, proximity ligation/extension assay, immunomagnetic reduction assay), to analyses of single cells producing cytokines (ELISpot, flow cytometry, mass cytometry and emerging techniques for single cell secretomics). Although this review is focused mainly on cancer and particularly melanoma, the discussed techniques are in general applicable to broad research field of biology and medicine, including stem cells, development, aging, immunology and intercellular communication.

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Section: Cytokine Detection Techniquesmentioning

confidence: 99%

Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research

Skalníková

Čížková

Cervenka

et al. 2017

IJMS

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“…The discovery of N ‐glycan biomarkers in serum could lead to clinical implementation for disease detection. The majority of serum N ‐glycan methods focus on the analysis of a pool of N ‐glycans released from all proteins in the serum (Gornik et al., ; Kirmiz et al., ; Novokmet et al., ; Reiding et al., ; Ruhaak et al., ; Ruhaak, Xu, Li, Goonatilleke, & Lebrilla, ), or the analysis of one target protein's N ‐glycan profile (Comunale et al., ; Pompach et al., ; Ruhaak et al., , ; Shubhakar et al., ; Simunovic et al., ; Šimurina et al., ; Theodoratou et al., ; Zhang et al., ). Pooled serum analyses have shown trends in overall N ‐glycan changes in the presence of cancer, such as increased fucosylation, branching, and bisects (Gebrehiwot et al., ; Hecht et al., ; Snyder et al., ; Vučković et al., ).…”

Section: Commentarymentioning

confidence: 99%

Antibody Panel Based N‐Glycan Imaging for N‐Glycoprotein Biomarker Discovery

et al. 2019

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Antibody panel based N‐glycan imaging is a novel platform for N‐glycan analysis of immunocaptured proteins. N‐glycosylation is a post‐translational modification of pathophysiological importance and is often studied in the context of disease biomarkers. Determination of protein‐specific N‐glycosylation changes in patient samples has traditionally been laborious or limited to study of a single protein per analysis. This novel technique allows for the multiplexed analysis of N‐glycoproteins from biofluids. Briefly, this platform consists of antibodies spotted in an array panel to a microscope slide, specific capture of glycoproteins from a biological sample, and then enzymatic release of N‐glycans for analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry (MS). N‐glycans are detected at each individual spot, allowing N‐glycan information to easily be linked back to its protein carrier. Using this protocol, multiplexed analysis of N‐glycosylation on serum glycoproteins can be performed. Human serum is discussed here, but this method has potential to be applied to other biofluids and to any glycoprotein that can be captured by a validated antibody. © 2019 by John Wiley & Sons, Inc. Basic Protocol: Antibody panel based N‐glycan imaging by MALDI MS Support Protocol: Confirmation of antibody capture by IR‐labeled proteins

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“…An early manifestation of this approach involved on‐plate digestion as a rapid method for the analysis and identification of bacterial proteins . In another practical illustration of the potential of lab‐on‐a‐plate, Pompach et al report high‐throughput, practical haptoglobin phenotyping based on MALDI plates functionalized with antihaptoglobin antibodies applied to the surface.…”

Section: Variations On the Ldi Theme: Multiplexed Combinatorial Andmentioning

confidence: 99%

The ongoing evolution of laser desorption/ionization mass spectrometry: Some observations on current trends and future directions

Muthu

Chun

et al. 2018

J Mass Spectrom

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The practice of laser desorption/ionization (LDI) mass spectrometry continues to evolve. In the most commonly adopted manifestation of LDI, matrix assisted LDI, attention continues to be directed towards novel sample application strategies and modifications to the sample plate. Specifically, researchers continue to explore adaptations to the conventional, stainless steel sample plate that is the centerpiece of conventional LDI. Numerous variants of LDI-MS have been reported based on modifications of the plate surface, but none of these is widely adopted, either by end-users or by instrument manufacturers. Further, at this time, advances in surface engineering have had only modest impact on day-to-day operation. In this article, we review and discuss some of the numerous, but scattered reports on novel LDI strategies with an emphasis on modified sample support substrates and plates. We discuss and highlight innovations that have the potential to markedly enhance the utility of LDI-MS.

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Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry

Cited by 19 publications

References 37 publications

Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research

Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research

Antibody Panel Based N‐Glycan Imaging for N‐Glycoprotein Biomarker Discovery

The ongoing evolution of laser desorption/ionization mass spectrometry: Some observations on current trends and future directions

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