Planar Electrochromatography in a Closed System under Pressure—Pressurized Planar Electrochromatography

Abstract

Pressurized planar electrochromatography (PPEC) is described taking into account last achievements in this mode such as construction of the device and variables influencing on separation efficiency (mobile phase composition, pH of the mobile phase, buffer concentration, applied voltage, chromatographic plate preparation, chromatographic plate type and temperature).

Separation of Some Aromatic Amino Acid Enantiomers with Pressurized Planar Electrochromatography and TLC

“…It is apparent that increase of buffer concentration leads to increase of migration distance of the solutes. This effect is known for capillary electrochromatography [30] and planar electrochromatography [13,20,27] and was explained by diminution of overlapping of electrical double layers with increase of buffer concentration [31].…”

“…It is reasonable, because electroosmotic flow velocity of the mobile phase in such systems demonstrates diminution of its value with pH decrease for PPEC [18,20] and for planar electrochromatography in open system (PEC) [27]. It seems that this effect and complete dissociation of the basic group of the solutes are responsible for poor separation selectivity of amino acids investigated, with the exception of S-Tyr and S-Phe, at lower pH value, 3.0, of buffer in the mobile phase.…”

“…PPEC is relatively new mode of separation in which the mobile phase is driven into movement by electroosmotic effect in closed separation system [13][14][15][16][17][18][19][20][21][22][23][24][25]. This movement can be easily controlled by electric field strength and mobile phase composition (pH of the mobile phase, buffer concentration, organic solvent type and its concentration) [13][14][15][16][17][18][19][20].…”

“…This movement can be easily controlled by electric field strength and mobile phase composition (pH of the mobile phase, buffer concentration, organic solvent type and its concentration) [13][14][15][16][17][18][19][20].…”

“…The development of PPEC has been practically reported by three research groups [13][14][15][16][17][18][19][20][21][22][23][24][25] so far. In these papers, few distinct features of PPEC such as high efficiency, short time of separation process and different separation selectivity have been presented relative to thin-layer chromatography/high-performance thin-layer chromatography (TLC/HPTLC).…”