Planar Chromatographic Method Development Using the PRISMA Optimization System and Flow Charts

Nyiredy, Sz.

doi:10.1093/chromsci/40.10.553

Cited by 36 publications

(18 citation statements)

References 27 publications

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“…[9,10] A combination of diethyl ether, 1,4-dioxan, and ethanol at a selectivity point (P S ) of 333 was selected. The solvent strength (S T ) was adjusted with n-hexane to 0.8, resulting in a mobile phase consisting of 78.4% n-hexane, 9.7% diethyl ether, 5.6% 1,4-dioxan, and 6.3% ethanol.…”

Section: Resultsmentioning

confidence: 99%

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Comparison of Medium Pressure Solid–Liquid Extraction and Rotation Planar Extraction of Ficus Leaves with Reference to Optimum Operating Parameters

Yrjönen

Vovk

Simonovska

et al. 2003

Journal of Liquid Chromatography & Related Technologies

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The effect of three operating variables on the extraction efficiency of medium pressure solid-liquid extraction (MPSLE) was studied using a 3289 factorial experimental design with three levels. The operating variables tested were medium particle size of the plant material, volume of extractant, and the equilibrium time between consecutive extractions of the same material. The possibility of scaling up the MPSLE extraction process on larger columns was also confirmed. The extraction efficiency did not markedly decrease, and a systematic increase in the extraction yields was observed on scaling up the MPSLE procedure. The results of the MPSLE experiments were compared with those obtained by the rotation planar extraction (RPE) method using a prototype ExtraChrom 1 separation instrument with particle size of the plant material and volume of extractant as the operating variables. Both methods proved to be suitable for the efficient extraction of the leaves of various Ficus species. The strong points of RPE were the ease of operation of the ExtraChrom 1 separation instrument and significant time savings achieved, whereas the possibility of scaling up the extraction process made MPSLE a suitable method for even larger scale preparative extractions.

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Section: Resultsmentioning

confidence: 99%

“…[9,10] TLC was performed on Kieselgel F 254 TLC plates (Merck, Germany). n-Hexane was of technical grade (Oy Exxon Chemicals Ab, Finland) and was filtered prior to use.…”

Section: Tlc Conditionsmentioning

confidence: 99%

Comparison of Medium Pressure Solid–Liquid Extraction and Rotation Planar Extraction of Ficus Leaves with Reference to Optimum Operating Parameters

Yrjönen

Vovk

Simonovska

et al. 2003

Journal of Liquid Chromatography & Related Technologies

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“…The PRISMA method can be geometrically represented by a prism that has three vertical lengths presenting the solvent strengths of selected solvents (Fig. 1II) is the region where the optimum point is actually situated (Nyiredy, 2002). 1(II).…”

Section: Prisma Optimization Of Mobile Phasementioning

confidence: 99%

“…'PRISMA' optimization was carried out using the method described by Nyiredy (2002) with modification. First, nine experiments were run in TLC using nine groups of solvent as described by Snyder solvent classification (Snyder, 1978).…”

Section: Prisma Optimizationmentioning

confidence: 99%

Multivariate analysis of PRISMA optimized TLC image for predicting antioxidant activity and identification of contributing compounds from Pereskia bleo

Khan

Rahman

Azmir

et al. 2015

Biomedical Chromatography

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Multivariate analysis of thin-layer chromatography (TLC) images was modeled to predict antioxidant activity of Pereskia bleo leaves and to identify the contributing compounds of the activity. TLC was developed in optimized mobile phase using the 'PRISMA' optimization method and the image was then converted to wavelet signals and imported for multivariate analysis. An orthogonal partial least square (OPLS) model was developed consisting of a wavelet-converted TLC image and 2,2-diphynyl-picrylhydrazyl free radical scavenging activity of 24 different preparations of P. bleo as the x- and y-variables, respectively. The quality of the constructed OPLS model (1 + 1 + 0) with one predictive and one orthogonal component was evaluated by internal and external validity tests. The validated model was then used to identify the contributing spot from the TLC plate that was then analyzed by GC-MS after trimethylsilyl derivatization. Glycerol and amine compounds were mainly found to contribute to the antioxidant activity of the sample. An alternative method to predict the antioxidant activity of a new sample of P. bleo leaves has been developed.

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“…The suggested solvents used for extraction were characterized on the basis of the Snyder theory [9]. The individual solvent strength and selectivity values of the solvents have been used to formulate an extraction strategy by use of selected solvents, in a manner similar to that used for the 'PRISMA' mobile phase optimization procedures [10]. The structures and properties of the compounds to be extracted do not have to be known for the procedure.…”

Section: Introductionmentioning

confidence: 99%

Medium-pressure solid-liquid extraction: A new preparative method based on the principle of counter-current

Nyiredy¹,

Botz

2003

Chromatographia

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SummaryA novel variant of continuous relative counter-current extraction, called medium-pressure solid-liquid extraction (MPSLE) is introduced as a new preparative separation method for laboratory purposes. This method is a special case of exhaustive extraction.On using MPSLE, the extraction column, i.e. a medium pressure liquid chromatographic (MPLC) column is filled with the fine-powdered solid phase to be extracted, and extraction solvent(s) is/are forced through the stationary bed by means of a pump. This method constitutes the relative counter-current extraction, and results in exhaustive and rapid extraction. By MPSLE, efficiency is achieved not just because the extraction solvent(s) is/are forced through the staying material to be extracted, but also clue to the overpressure in the extraction column and the suitable changes of the dissolution and diffusion periods of the process.The method can be used for the rapid extraction of various substance classes occurring as complex solid matrices. Experiments carried out with MPLC columns show that the exhaustive extraction of finely powdered plant material of 100-3000g can be extracted and concentrated with an automated equipment within a few hours. Further advantages of the environment-friendly process performed in closed system are full automation and operation with low qua ntil',/of extracta nt.

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Planar Chromatographic Method Development Using the PRISMA Optimization System and Flow Charts

Cited by 36 publications

References 27 publications

Comparison of Medium Pressure Solid–Liquid Extraction and Rotation Planar Extraction of Ficus Leaves with Reference to Optimum Operating Parameters

Comparison of Medium Pressure Solid–Liquid Extraction and Rotation Planar Extraction of Ficus Leaves with Reference to Optimum Operating Parameters

Multivariate analysis of PRISMA optimized TLC image for predicting antioxidant activity and identification of contributing compounds from Pereskia bleo

Medium-pressure solid-liquid extraction: A new preparative method based on the principle of counter-current

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