Planar cell polarity: the <i>prickle</i> gene acts independently on both the Ds/Ft and the Stan/Fz systems

Casal, José; Ibanez-Jimenez, Beatriz; Lawrence, Peter A.

doi:10.1242/dev.168112

Cited by 9 publications

(9 citation statements)

References 48 publications

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“…Another intriguing possibility is that the Wnt pathway genes might act through the Fat-Dachsous (Ft-Ds) pathway, a possibility not discussed in the literature thus far. In flies, disruptions in Ft-Ds signaling cause swirling wing hair patterns and disruptions of the global alignment of PCP proteins without affecting their asymmetric distribution, reminiscent of the phenotypes we observed in Wnt pathway mutants (Casal et al, 2018;Casal et al, 2006;Devenport, 2014;Gubb and Garcia-Bellido, 1982;Matis and Axelrod, 2013;Thomas and Strutt, 2012). Furthermore, a recent report shows that the Ft-Ds pathway directs the uniform axial orientation of cells in the Drosophila abdomen (Mangione and Martin-Blanco, 2018), which may be similar to the coordinated organization observed in support cells in our study.…”

Section: A Pcp-independent Mechanism For Support Cell Organizationmentioning

confidence: 55%

Parallel control of mechanosensory hair cell orientation by the PCP and Wnt pathways

Acedo

Voas

Richard

et al. 2019

Preprint

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Cell polarity plays a crucial role during development of vertebrates and invertebrates.Planar Cell Polarity (PCP) is defined as the coordinated polarity of cells within a tissue axis and is essential for processes such as gastrulation, neural tube closure or hearing. Wnt ligands can be instructive or permissive during PCP-dependent processes, and Wnt pathway mutants are often classified as PCP mutants due to the complexity and the similarities between their phenotypes.Our studies of the zebrafish sensory lateral line reveal that disruptions of the PCP and Wnt pathways have differential effects on hair cell orientations. While mutations in PCP genes cause random orientations of hair cells, mutations in Wnt pathway members induce hair cells to adopt a concentric pattern. We show that PCP signaling is normal in hair cells of Wnt pathway mutants and that the concentric hair cell phenotype is due to altered organization of the surrounding support cells. Thus, the PCP and Wnt pathways work in parallel, as separate pathways to establish proper hair cell orientation. Our data suggest that coordinated support cell organization is established during the formation of lateral line primordia, much earlier than the appearance of hair cells. Together, these finding reveal that hair cell orientation defects are not solely explained by defects in PCP signaling and that some hair cell phenotypes warrant reevaluation.

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Section: A Pcp-independent Mechanism For Support Cell Organizationmentioning

confidence: 55%

Parallel control of mechanosensory hair cell orientation by the PCP and Wnt pathways

Acedo

Voas

Richard

et al. 2019

Preprint

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“…The many experiments and contrasting interpretations in this area are well presented by Strutt and Strutt [51]. The recent intervention of the Pk gene into this melée has not simplified that debate [35, 52–55]. However, our results above (those comparing fz - expressing clones in abdomens with and without Fj, figure 4, where we ask one PCP system to act against the other), are simply explained if the systems act independently.…”

Section: Discussionmentioning

confidence: 74%

Planar cell polarity: intracellular asymmetry and supracellular gradients of Dachsous

Chorro

Verma

Homfeldt

et al. 2022

Preprint

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The slope of a supracellular molecular gradient has long been thought to orient and coordinate planar cell polarity (PCP). Here we demonstrate and measure that gradient. Dachsous (Ds) is a conserved and elemental molecule of PCP; Ds forms intercellular bridges with another cadherin molecule, Fat (Ft), an interaction modulated by the Golgi protein Four-jointed (Fj). Using genetic mosaics and tagged Ds we measure Ds in vivo in membranes of individual cells over a whole metamere of the Drosophila abdomen. We find: 1. A supracellular gradient that rises from head to tail in the anterior compartment (A) and then falls in the posterior compartment (P). 2. There is more Ds in the front than the rear membranes of all cells in the A compartment, except that compartment's most anterior and most posterior cells. There is more Ds in the rear than in the front membranes of all cells of the P compartment 3. The loss of Fj removes intracellular asymmetry anteriorly in the segment and reduces it elsewhere. Additional experiments show that Fj makes PCP more robust. Using Dachs (D) as a molecular indicator of polarity, we confirm that opposing gradients of PCP meet slightly out of register with compartment boundaries.

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“…(4) Ft and Ds can independently control polarity in the abdomen. Clones of cells overexpressing Ft can reverse the polarity of hairs in adjacent wild-type tissue, even in the absence of Fmi or Fz activity [107,117]. If Ft and Ds were strictly upstream of the core proteins, then Ft-Ds would not be able to repolarize hairs in their absence (see §7).…”

Section: Evidence Against a Sequential Action Modelmentioning

confidence: 99%

“…Experiments in the abdomen have shown that Ft and Ds can polarize hairs and bristles independently of the core proteins: for instance clones overexpressing Ft can repolarize wild-type cells lacking Fmi or Fz [107,117]. Thus while the core proteins may be the primary cue for hair orientation in this tissue, Ft-Ds can orient hairs in their absence.…”

Section: Polarization Of Cuticular Structures Bymentioning

confidence: 99%

How do the Fat–Dachsous and core planar polarity pathways act together and independently to coordinate polarized cell behaviours?

Strutt

2021

Open Biol.

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Planar polarity describes the coordinated polarization of cells within the plane of a tissue. This is controlled by two main pathways in Drosophila : the Frizzled-dependent core planar polarity pathway and the Fat–Dachsous pathway. Components of both of these pathways become asymmetrically localized within cells in response to long-range upstream cues, and form intercellular complexes that link polarity between neighbouring cells. This review examines if and when the two pathways are coupled, focusing on the Drosophila wing, eye and abdomen. There is strong evidence that the pathways are molecularly coupled in tissues that express a specific isoform of the core protein Prickle, namely Spiny-legs. However, in other contexts, the linkages between the pathways are indirect. We discuss how the two pathways act together and independently to mediate a diverse range of effects on polarization of cell structures and behaviours.

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Planar cell polarity: the prickle gene acts independently on both the Ds/Ft and the Stan/Fz systems

Cited by 9 publications

References 48 publications

Parallel control of mechanosensory hair cell orientation by the PCP and Wnt pathways

Parallel control of mechanosensory hair cell orientation by the PCP and Wnt pathways

Planar cell polarity: intracellular asymmetry and supracellular gradients of Dachsous

How do the Fat–Dachsous and core planar polarity pathways act together and independently to coordinate polarized cell behaviours?

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