Plain Silver Surface Plasmon Resonance for Microarray Application

Abstract

The application scope of surface plasmon resonance (SPR) and SPR imaging (SPRi) is rapidly growing, and tools such as high-performance and low-cost slides could enable more rapid growth of the field. We describe herein a novel silver slide, addressing the inherent instability of plain silver structure by improving adhesion between the glass substrate and the silver layer with a thin buffer layer of gold. Covered by a self-assembled monolayer (SAM) only, SPR characteristics of the slide remain steady for more than 3 months under regular storage. In a bioassay, the slide substantiates the predicted nearly 100% sensitivity improvement over gold slides and exhibits exceptional performance stability as determined by sensitivity and resolution measurements during the extended 40,000 s multicycle experiment. We demonstrate the suitability of this new slide for large-area SPRi, describing analysis results from a 1 296-ligand protein microarray. We predict this slide structure will provide a stable, high-sensitivity solution for high-throughput SPRi applications and other surface analysis platforms.

Sensitivity Enhancement of a Surface Plasmon Resonance Sensor with Platinum Diselenide

“…Biomolecules and metallic membranes can interact with each other, through which biomolecules can be detected. We have used Ag or Au to cover, on the basis of the optical coupling prism, BK7 glass to be used as the coupling prism, and PtSe 2 covers the metallic layer in this structure [34,35].…”

A Sensitive and Stable Surface Plasmon Resonance Sensor Based on Monolayer Protected Silver Film

“…However, the poor chemical stability limits its application. So far, methods to protect silver film from oxidation and sulfuration mainly involve the use of bimetallic silver/gold layers [ 12 , 13 , 14 , 15 , 16 , 17 ], deposition of dielectric thin films [ 18 , 19 , 20 , 21 , 22 , 23 , 24 ] or polymers [ 25 ], and the use of self-assembled monolayers (SAM) [ 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. In the structure of silver/gold film, the silver was protected by an overlay of ca.15 nm-thick gold film [ 14 ], which is directly connected with the air or the sample solution.…”

Automatic Spot Identification Method for High Throughput Surface Plasmon Resonance Imaging Analysis

“…The line section of Figure 3 a is shown in Figure 4 a. The curvature of the baseline in Figure 4 a indicates that light was nonuniform through the image [ 28 ]. Besides, irregular and unclear contours of the spots and slide contamination can be seen in the figure.…”

“…Fabrication of gold slides, preparation of FKBP12 and rabbit IgG microarrays and SPRi measurements were implemented according to our previous works [ 27 , 28 ]. Briefly, the gold slides were modified by thiol-poly(ethylene glycol acid) by incubation in 4 C overnight and then activated by the sulfo-NHS/EDC mixture (0.1 M and 0.2 M, respectively).…”