Placental transfer of melamine and its effects on rat dams and fetuses

Wang, Jingbin; Ndong, Moussa; Hao, Kai; Matsuno, Koji; Kayama, Fujio

doi:10.1016/j.fct.2010.03.043

Cited by 49 publications

(27 citation statements)

References 18 publications

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“…Both transfer routes through placenta or milk were validated by other studies1819. Importantly, we showed that melamine is able to retain in the offspring after 3 months, indicating a potential long-term renal damage or likely a predisposition to other kidney diseases.…”

Section: Discussionsupporting

confidence: 71%

Melamine Impairs Renal and Vascular Function in Rats

Tian

Wong

Lau

et al. 2016

Sci Rep

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Melamine incident, linked to nephrotoxicity and kidney stone in infants previously exposed to melamine-contaminated milk products, was unprecedentedly grave in China in 2008 as little was known about the mechanistic process leading to renal dysfunction in affected children. This study investigates whether neonatal ingestion of melamine leads to renal and vascular dysfunction in adulthood; and whether ingestion of melamine in pregnant rats leads to renal dysfunction in their offspring. A combination of approaches employed includes functional studies in rat renal arteries, renal blood flow measurement by functional magnetic resonance imaging, assay for pro-inflammatory and fibrotic biomarkers, immunohistochemistry, and detection of plasma and renal melamine. We provide mechanistic evidence showing for the first time that melamine reduces renal blood flow and impairs renal and vascular function associated with overexpression of inflammatory markers, transforming growth factor-β1, bone morphogenic protein 4 and cyclooxygenase-2 in kidney and renal vasculature. Melamine also induces renal inflammation and fibrosis. More importantly, melamine causes nephropathies in offsprings from pregnant rat exposed to melamine during pregnancy, as well as in neonatal rat exposed to melamine afterbirth, thus supporting the clinical observations of kidney stone and acute renal failure in infants consuming melamine-contaminated milk products.

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Section: Discussionsupporting

confidence: 71%

Melamine Impairs Renal and Vascular Function in Rats

Tian

Wong

Lau

et al. 2016

Sci Rep

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“…Placenta samples were analyzed using liquid–liquid extraction (LLE), SPE and isotope‐dilution LC/MS/MS . Briefly, placenta samples were added to 13 C 3 15 N 3 ‐melamine‐labeled solution as an IS, homogenized with 2.5% HCl, and centrifuged at 3500 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…In mammals, mother and fetal compartments are separated by the placenta, an organ that connects the developing fetus to the uterine wall and the only source of nutrients from the pregnant mother . Animal studies, which have detected melamine in the placentas, amniotic fluid, and even fetus or neonatal kidney in rats, have concluded that melamine can cross the placental barrier and enter the fetus …”

Section: Introductionmentioning

confidence: 99%

Development of analytical method of melamine in placenta from pregnant women by isotope‐dilution liquid chromatography/tandem mass spectrometry

Cheng

Hsu

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Melamine is ubiquitously present in our daily life. It has a known effect on the kidneys, but it may also adversely affect the reproduction system. We have developed an analytical method for measuring melamine levels in maternal placenta and correlated these levels with melamine concentrations in urine, a necessary step in finding out if melamine might cross the placenta and enter the circulation of the fetus. Methods We used liquid–liquid extraction, clean up by solid‐phase extraction (SPE), and isotope‐dilution liquid chromatography/tandem mass spectrometry (LC/MS/MS) to measure melamine in placenta specimens. The results of this method were assessed for linearity, limits of quantitation (LOQs), and intra‐ and inter‐assay precision as well as accuracy, matrix effect, and recovery rate. Results Calibration curves indicated good linearity (r >0.995) over concentrations ranging from 5 to 500 ng/mL in placenta specimens, intra‐ and inter‐assay precision from 0.89% to 27.07%, and accuracy from 92.4% to123.5%. Recovery ranged from 63.9 to 83.9%, and the LOQ was 5 ng/mL in placenta (0.2 g). Placental melamine levels ranged from 7.87 to19.64 ng/mL, all detectable (n = 8). Pregnant women with higher levels of urinary melamine had higher placenta melamine levels than those with non‐detectable urinary melamine, though the results were not significantly different (p = 0.149, n = 4 in each group). Conclusions The results of this study suggest that pregnant women were exposed to low doses of melamine in their daily lives as measured in urine samples and placenta specimens. It is unclear whether placenta melamine concentrations can better represent long‐term exposure than urine or whether melamine in the uterus can enter the fetus via this route.

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“…Antibiotics are widely adopted to kill or inhibit microorganisms in food products to maintain the quality or elongate the shelf-life, while the abuse of them would result in various side effects in human and even the appearance of super bacteria with tolerance to related antibiotics [66, 67]. Song et al [68] have carried out a gold nanoparticle-based colorimetric method for kanamycin using aptamer probe.…”

Section: Application Of Aptamers In Food Safety Monitoringmentioning

confidence: 99%

Recent trends in SELEX technique and its application to food safety monitoring

Zhu

Xue

et al. 2014

Microchim Acta

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The method referred to as “systemic evolution of ligands by exponential enrichment” (SELEX) was introduced in 1990 and ever since has become an important tool for the identification and screening of aptamers. Such nucleic acids can recognize and bind to their corresponding targets (analytes) with high selectivity and affinity, and aptamers therefore have become attractive alternatives to traditional antibodies not the least because they are much more stable. Meanwhile, they have found numerous applications in different fields including food quality and safety monitoring. This review first gives an introduction into the selection process and to the evolution of SELEX, then covers applications of aptamers in the surveillance of food safety (with subsections on absorptiometric, electrochemical, fluorescent and other methods), and then gives conclusions and perspectives. The SELEX method excels by its features of in vitro, high throughput and ease of operation. This review contains 86 references.

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Placental transfer of melamine and its effects on rat dams and fetuses

Cited by 49 publications

References 18 publications

Melamine Impairs Renal and Vascular Function in Rats

Melamine Impairs Renal and Vascular Function in Rats

Development of analytical method of melamine in placenta from pregnant women by isotope‐dilution liquid chromatography/tandem mass spectrometry

Recent trends in SELEX technique and its application to food safety monitoring

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