Placental stem cells pre-treated with a hyaluronan mixed ester of butyric and retinoic acid to cure infarcted pig hearts: a multimodal study

Simioniuc, Anca; Campan, M.; Lionetti, Vincenzo; Marinelli, Martina; Aquaro, Giovanni Donato; Cavallini, Claudia; Valente, Sabrina; Silvestre, Dario Di; Cantoni, Silvia; Bernini, Fabio; Simi, Claudia; Pardini, S.; Mauri, Pierluigi; Neglia, Danilo; Ventura, Carlo; Pasquinelli, Gianandrea; Recchia, Fabio A.

doi:10.1093/cvr/cvr018

Cited by 56 publications

(50 citation statements)

References 38 publications

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“…Recently, it has been indicated that NaBu attenuated gentamicin-induced nephrotoxicity by enhancing renal antioxidant enzyme activity and expression of prohibitin protein (Sun et al, 2013). Moreover, hyaluronan mixed esters of butyric and retinoic acid have also been shown to enhance cardiac repair in infarcted rat and pig hearts (Ventura et al, 2007;Simioniuc et al, 2011). Previously, we have reported that ATRA induces Npr1 gene transcription in cultured mouse mesangial cells (MMCs) in association with E26 transformation-specific 1 (Ets-1), specificity protein 1 (Sp1), and histone acetylation (Kumar et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

All-Trans Retinoic Acid and Sodium Butyrate Enhance Natriuretic Peptide Receptor A Gene Transcription: Role of Histone Modification

et al. 2014

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The objective of the present study was to delineate the mechanisms of GC-A/natriuretic peptide receptor-A (GC-A/ NPRA) gene (Npr1) expression in vivo. We used all-trans retinoic acid (ATRA) and histone deacetylase (HDAC) inhibitor, sodium butyrate (NaBu) to examine the expression and function of Npr1 using gene-disrupted heterozygous (1-copy; 1/2), wild-type (2-copy; 1/1), and gene-duplicated heterozygous (3-copy; 11/1) mice. Npr1 1/2 mice exhibited increased renal HDAC and reduced histone acetyltransferase (HAT) activity; on the contrary, Npr111/1 mice showed decreased HDAC and enhanced HAT activity compared with Npr1 1/1 mice. ATRA and NaBu promoted global acetylation of histones H3-K9/14 and H4-K12, reduced methylation of H3-K9 and H3-K27, and enriched accumulation of active chromatin marks at the Npr1 promoter. A combination of ATRA-NaBu promoted recruitment of activator-complex containing E26 transformation-specific 1, retinoic acid receptor a, and HATs (p300 and p300/cAMP response element-binding protein-binding protein-associated factor) at the Npr1 promoter, and significantly increased renal NPRA expression, GC activity, and cGMP levels. Untreated 1-copy mice showed significantly increased systolic blood pressure and renal expression of a-smooth muscle actin (a-SMA) and proliferating cell nuclear antigen (PCNA) compared with 2-and 3-copy mice. Treatment with ATRA and NaBu synergistically attenuated the expression of a-SMA and PCNA and reduced systolic blood pressure in Npr1 1/2 mice. Our findings demonstrate that epigenetic upregulation of Npr1 gene transcription by ATRA and NaBu leads to attenuation of renal fibrotic markers and systolic blood pressure in mice with reduced Npr1 gene copy number, which will have important implications in prevention and treatment of hypertension-related renal pathophysiological conditions.

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Section: Introductionmentioning

confidence: 99%

All-Trans Retinoic Acid and Sodium Butyrate Enhance Natriuretic Peptide Receptor A Gene Transcription: Role of Histone Modification

et al. 2014

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“…Inhibition of HDACs by RA and SB has also been shown to improve cardiovascular and renal disease conditions (3,5,36). Moreover, mixed esters of butyric acid and retinoic acid hybrid drug (HB) have also been reported to enhance cardiac repair of infarcted hearts in the experimental animals (61,70). In the present study, we examined the effect of RA-, SB-, and HB-mediated effects on the study (30).…”

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confidence: 96%

Retinoic acid and sodium butyrate suppress the cardiac expression of hypertrophic markers and proinflammatory mediators in Npr1 gene-disrupted haplotype mice

Umadevi

Kumar

Mani

et al. 2016

Physiological Genomics

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“…This finding points at the relevant pleiotropic role of hyaluronic acid (HA) and glycosaminoglycans in the intracrine regulation of cell polarity and at the chance of using physical energies to preserve and direct this fundamental attribute of Life. HA has been used as a component to promote cardiogenesis in mouse embryonic [42] and human adult stem cells of different origin in vitro and in vivo [43][44][45] and to induce cardiac repair in vivo without stem cell transplantation in a rat model of myocardial infarction [46]. Accordingly, HAS2 suppression abolished the capability of human ES cells to differentiate in vitro along the cardiogenic and vasculogenic lineages [47].…”

Section: S4mentioning

confidence: 99%

Seeing Cell Biology with the Eyes of Physics

Ventura¹

2017

NanoWorld J

Self Cite

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Rhythmic oscillatory patterns permeate the entire universe and sustain cellular dynamics at biological level. The intracellular environment is now regarded as a complex nanotopography embedding "intracrine" signals that encompass the intracellular action of regulatory molecules coupled with the newly discovered functions of the microtubuar network. Microtubuli, are far away from simply being a part of the cytoskeleton, since they produce both nanomechanical motions and display electromagnetic resonance modes that may turn local events into non-local, long-ranging paths. The possibility that microtubuli form a bioelectronic circuit prompts rethinking of biomolecular recognition as a result of synchronization of the oscillatory patterns of proteins sustained and orchestrated by resonance modes brought about by the microtubular network itself. Here, we discuss these issues within the biomedical perspective of using physical energies to govern (stem) cell fate. We focus on the ability of specially conveyed electromagnetic fields to afford optimization of stem cell polarity and pluripotency, reversing stem cell aging and promoting a multilineage repertoire in human adult stem cells, as well as in human non-stem somatic cells. We discuss the use atomic force microscopy and hyperspectral imaging for deciphering the nanomotions generated by (stem) cells during their growth and differentiation. We highlight the potential for unraveling vibrational signatures that can be exploited to direct the differentiation and self-healing potential of tissue-resident stem cells in vivo. In conclusion, seeing stem cell biology with the eyes of Physics may help developing a Regenerative/Precision medicine afforded through the stimulation of the natural ability of tissues for self-healing, without the needs of stem cell transplantation.

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Placental stem cells pre-treated with a hyaluronan mixed ester of butyric and retinoic acid to cure infarcted pig hearts: a multimodal study

Abstract: Our results support a more pronounced reparative potential of HBR-pre-treated FMhMSCs in a clinically relevant animal model of infarction and highlight the necessity of using combined diagnostic imaging to avoid underestimations of stem cell therapeutic effects in the heart.

Cited by 56 publications

References 38 publications

All-Trans Retinoic Acid and Sodium Butyrate Enhance Natriuretic Peptide Receptor A Gene Transcription: Role of Histone Modification

All-Trans Retinoic Acid and Sodium Butyrate Enhance Natriuretic Peptide Receptor A Gene Transcription: Role of Histone Modification

Retinoic acid and sodium butyrate suppress the cardiac expression of hypertrophic markers and proinflammatory mediators in Npr1 gene-disrupted haplotype mice

Seeing Cell Biology with the Eyes of Physics

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