Placental plastics in young women from general population correlate with reduced foetal growth in IUGR pregnancies

Amereh, Fatemeh; Amjadi, Nooshin; Mohseni‐Bandpei, Anoushiravan; Isazadeh, Siavash; Mehrabi, Yadollah; Eslami, Akbar; Naeiji, Zahra; Rafiee, Mohammad

doi:10.1016/j.envpol.2022.120174

Cited by 55 publications

(78 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, a grating with 600 lines/mm was consistently used across all three studies. 31,42,72 Amereh et al 73 did not report microscope methodological parameters.…”

Section: Raman Spectroscopymentioning

confidence: 99%

“…The subsamples were either cut to a specific size (1 cm × 1 cm × 1 cm), 34 or cut from specific anatomical regions, e.g., maternal, fetal, or chorioamniotic membrane. 33,73 Among the various methods of chemical digestion used in studies, KOH was the most frequent, used for placenta, colon, thrombi, breast milk, and enclosed body fluids at concentrations of 10% or 30%. 31,33,37,42,72,73 H 2 O 2 (30%) and nitric acid (HNO 3 , 68%) have also been utilized, for lung/veinous tissue and sputum/BALF, respectively.…”

Section: External Exposurementioning

confidence: 99%

“…33,73 Among the various methods of chemical digestion used in studies, KOH was the most frequent, used for placenta, colon, thrombi, breast milk, and enclosed body fluids at concentrations of 10% or 30%. 31,33,37,42,72,73 H 2 O 2 (30%) and nitric acid (HNO 3 , 68%) have also been utilized, for lung/veinous tissue and sputum/BALF, respectively. 44,69,70 Additionally, enzymatic digestion has been employed in a study analyzing testis and semen.…”

Section: External Exposurementioning

confidence: 99%

“…Investigations on microplastic levels in purified human tissue samples commonly utilize FT-IR (66.6%); 33,34,37,40,44,[69][70][71] and Raman (33.3%). 31,42,72,73 One study combined py-GC-MS with LD-IR analysis. 33 Below is a further description of instrument configurations used for the differing microscopybased interrogations of biological samples for the presence of microplastic.…”

Section: Internal Exposurementioning

confidence: 99%

“…Two other polymer types, PET, and PP, were identified in IUGR placenta samples, comprising 14.9% and 5.6%, respectively. 73 Guan et al 42 observed that the compositional distribution of plastics in enclosed body fluids varied. Utilizing the metadata available from the Supplementary Material, we calculated the relative proportions of microplastic polymers within each fluid compared to the total number of particulates, including non-plastics like graphite.…”

Section: Raman Spectroscopymentioning

confidence: 99%

See 4 more Smart Citations

Application of Infrared and Near-Infrared Microspectroscopy to Microplastic Human Exposure Measurements

Wright,

Levermore,

Ishikawa

2023

Appl Spectrosc

View full text Add to dashboard Cite

Microplastic pollution is a global issue for the environment and human health. The potential for human exposure to microplastic through drinking water, dust, food, and air raises concern, since experimental in vitro and in vivo toxicology studies suggest there is a level of hazard associated with high microplastic concentrations. However, to infer the likelihood of hazards manifesting in the human population, a robust understanding of exposure concentrations is needed. Infrared and near-infrared microspectroscopies have routinely been used to analyze microplastic in different exposure matrices (air, dust, food, and water), with technological advances coupling multivariate and machine learning algorithms to spectral data. This focal point article will highlight the application of infrared and Raman modes of spectroscopy to detect, characterize, and quantify microplastic particles, with a focus on human exposure to microplastic. Methodologies and state-of-the-art approaches will be reported and potential confounding variables and challenges in microplastic analysis discussed. The article provides an up-to-date review of the literature on microplastic exposure measurement using (near) infrared spectroscopies as an analytical tool, highlighting the recent advances in this rapidly advancing field.

show abstract

“…Additionally, a grating with 600 lines/mm was consistently used across all three studies. 31,42,72 Amereh et al 73 did not report microscope methodological parameters.…”

Section: Raman Spectroscopymentioning

confidence: 99%

Section: External Exposurementioning

confidence: 99%

Section: External Exposurementioning

confidence: 99%

Section: Internal Exposurementioning

confidence: 99%

Section: Raman Spectroscopymentioning

confidence: 99%

See 3 more Smart Citations

Application of Infrared and Near-Infrared Microspectroscopy to Microplastic Human Exposure Measurements

Wright,

Levermore,

Ishikawa

2023

Appl Spectrosc

View full text Add to dashboard Cite

show abstract

Impact of Micro‐ and Nano‐Plastics on Human Intestinal Organoid‐Derived Epithelium

Wang,

Iglesias‐Ledon,

Bishop

et al. 2024

Current Protocols

View full text Add to dashboard Cite

The development of patient‐derived intestinal organoids represents an invaluable model for simulating the native human intestinal epithelium. These stem cell‐rich cultures outperform commonly used cell lines like Caco‐2 and HT29‐MTX in reflecting the cellular diversity of the native intestinal epithelium after differentiation. In our recent study examining the effects of polystyrene (PS), microplastics (MPs), and nanoplastics (NPs), widespread pollutants in our environment and food chain, on the human intestinal epithelium, these organoids have been instrumental in elucidating the absorption mechanisms and potential biological impacts of plastic particles. Building on previously established protocols in human intestinal organoid culture, we herein detail a streamlined protocol for the cultivation, differentiation, and generation of organoid‐derived monolayers. This protocol is tailored to generate monolayers incorporating microfold cells (M cells), key for intestinal particle uptake but often absent in current in vitro models. We provide validated protocols for the characterization of MPs/NPs via scanning electron microscopy (SEM) for detailed imaging and their introduction to intestinal epithelial monolayer cells via confocal immunostaining. Additionally, protocols to test the impacts of MP/NP exposure on the functions of the intestinal barrier using transendothelial electrical resistance (TEER) measurements and assessing inflammatory responses using cytokine profiling are detailed. Overall, our protocols enable the generation of human intestinal organoid monolayers, complete with the option of including or excluding M cells, offering crucial techniques for observing particle uptake and identifying inflammatory responses in intestinal epithelial cells to advance our knowledge of the potential effects of plastic pollution on human gut health. These approaches are also amendable to the study of other gut‐related chemical and biological exposures and physiological responses due to the robust nature of the systems. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Human intestinal organoid culture and generation of monolayers with and without M cellsSupport Protocol 1: Culture of L‐WRN and production of WRN‐conditioned mediumSupport Protocol 2: Neuronal cell culture and integration into intestinal epitheliumSupport Protocol 3: Immune cell culture and integration into intestinal epitheliumBasic Protocol 2: Scanning electron microscopy: sample preparation and imagingBasic Protocol 3: Immunostaining and confocal imaging of MP/NP uptake in organoid‐derived monolayersBasic Protocol 4: Assessment of intestinal barrier function via TEER measurementsBasic Protocol 5: Cytokine profiling using ELISA post‐MP/NP exposure.

show abstract

Microplastic environmental behavior and health risk assessment: a review

Lei,

Ma,

Ding

et al. 2024

Environ Chem Lett

View full text Add to dashboard Cite

Placental plastics in young women from general population correlate with reduced foetal growth in IUGR pregnancies

Cited by 55 publications

References 67 publications

Application of Infrared and Near-Infrared Microspectroscopy to Microplastic Human Exposure Measurements

Application of Infrared and Near-Infrared Microspectroscopy to Microplastic Human Exposure Measurements

Impact of Micro‐ and Nano‐Plastics on Human Intestinal Organoid‐Derived Epithelium

Microplastic environmental behavior and health risk assessment: a review

Contact Info

Product

Resources

About