Placental function and structure at term is altered in broodmares fed with cereals from mid-gestation

Robles, Morgane; Peugnet, Pauline; Dubois, Cédric; Piumi, François; Jouneau, Luc; Bouchez, Olivier; Aubrière, Marie-Christine; Dahirel, Michèle; Aı̈oun, Josiane; Wimel, Laurence; Couturier-Tarrade, Anne; Chavatte‐Palmer, Pascale

doi:10.1016/j.placenta.2018.02.003

Cited by 13 publications

(7 citation statements)

References 43 publications

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“…Surface densities (Sv) and Volume fractions (Vv) of the different components of the allantochorion, i.e ., microcotyledons and allantois, were quantified by One stop stereology using the Mercator® software (ExploraNova, France 58 ). As previously described 59 , the components measured were part of the microcotyledonary region (haemotrophic trophoblast, microcotyledonary vessels, connective tissue and microcotyledons as the sum of all microcotyledonary components) and of the allantoic region (histotrophic trophoblast and allantoic vessels. The chorionic mesoderm and allantoic connective tissue were merged as allantoic connective tissue).…”

Section: Placental Analysesmentioning

confidence: 99%

Effects of dietary arginine supplementation in pregnant mares on maternal metabolism, placental structure and function and foal growth

Robles

Couturier-Tarrade

Derisoud

et al. 2019

Sci Rep

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Foals born to primiparous mares are lighter and less mature than those born to multiparous dams. Factors driving this difference are not totally understood. Using 7 multiparous and 6 primiparous standardbred mares, we demonstrated that, in late gestation, primiparous mares were less insulin resistant compared to multiparous mares, and that their foals had reduced plasma amino-acid concentrations at birth compared to foals born to multiparous mares. Vascular development, as observed through structure and gene expression, and global DNA methylation were also reduced in primiparous placentas. Another group of 8 primiparous mares was orally supplemented with L-arginine (100 g/day, 210d to term). L-arginine improved pregnancy-induced insulin resistance and increased maternal L-arginine and L-ornithine plasma concentrations but foal plasma amino acid concentrations were not affected at birth. At birth, foal weight and placental biometry, structure, ultra-structure and DNA methylation were not modified. Placental expression of genes involved in glucose and fatty acid transfers was increased. In conclusion, maternal insulin resistance in response to pregnancy and placental function are reduced in primiparous pregnancies. Late-gestation L-arginine supplementation may help primiparous mares to metabolically adapt to pregnancy and improve placental function. More work is needed to confirm these effects and ascertain optimal treatment conditions.

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Section: Placental Analysesmentioning

confidence: 99%

Effects of dietary arginine supplementation in pregnant mares on maternal metabolism, placental structure and function and foal growth

Robles

Couturier-Tarrade

Derisoud

et al. 2019

Sci Rep

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“…An increase in allantoic arteriole vascular wall thickness (Fig. 4D) and a decrease in microcotyledonary vessels volume have been observed in term placentas of mares fed with cereals (flattened barley, 3.5 kg/day, last 4.5 months of gestation) compared to mares fed with forages only (Robles et al 2018e). These vascular alterations were also linked to modifications in the enrichment of gene sets involved in placental vascularization, inflammation, coagulation and collagen production in mares fed with concentrates (Robles et al 2018e).…”

Section: Effect Of Maternal Nutrition and Metabolismmentioning

confidence: 73%

“…4D) and a decrease in microcotyledonary vessels volume have been observed in term placentas of mares fed with cereals (flattened barley, 3.5 kg/day, last 4.5 months of gestation) compared to mares fed with forages only (Robles et al 2018e). These vascular alterations were also linked to modifications in the enrichment of gene sets involved in placental vascularization, inflammation, coagulation and collagen production in mares fed with concentrates (Robles et al 2018e). Placental inflammation may therefore also be involved in the mediation of the effects of cereal feeding in broodmares on the development of osteoarticular diseases in foals.…”

Section: Effect Of Maternal Nutrition and Metabolismmentioning

confidence: 88%

“…Maternal moderate undernutrition neither seem to affect foals birthweight nor placental measurements and efficiency (Henneke et al 1984, Peugnet et al 2015. Despite the lack of effect on placental gross morphology at term, placentas from slightly underfed mares fed with forage only (very poor in soluble carbohydrates but rich in fibres) had an enrichment in gene sets involved in amino acids, folate and anions transport, and amino acids, fatty acids, cholesterol and folate catabolism compared to mares fed at 100% of their needs but with concentrates in addition to forage (Robles et al 2018e). This observation indicates that placental metabolism is able to adapt to the source of energy.…”

Section: Effects Of Maternal Undernutritionmentioning

confidence: 91%

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Environmental constraints and pathologies that modulate equine placental genes and development

et al. 2022

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Equine placental development is a long process with unique features. Implantation occurs around 40 days of gestation (dpo) with the presence of a transient invasive placenta from 25-35dpo to 100-120dpo. The definitive non-invasive placenta remains until term (330d). This definitive placenta is diffuse and epitheliochorial, exchanging nutrients, gas and waste with the endometrium through microvilli, called microcotyledons. These are lined by an external layer of haemotrophic trophoblast. Moreover, histotrophic exchange remains active through the histotrophic trophoblast located along the areolae. Placental development is dependent on the maternal environment that can be affected by several factors (e.g., nutrition, metabolism, age, embryo technologies, pathologies) that may affect foetal development as well as long-term offspring health. The first section of the review focuses on normal placental development as well as definitive placental structure. Differences between the various areas of the placenta are also highlighted. The latter sections provide an overview of the effects of the maternal environment and reproductive pathologies, respectively, on trophoblast/placental gene expression and structure. So far, only pre-implantation and late gestation/term data are available, which demonstrate an important placental plasticity in response to environmental variation, with genes involved in oxidative stress and tissue differentiation mostly involved in the pre-implantation period, whereas genes involved in foeto-placental growth and nutrient transfers are mostly perturbed at term.

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“…Studies using microarrays and next generation sequencing (NGS) to investigate gene expression in equine placental tissue extend from descriptions of the primary derivative membrane, the blastocyst-derived trophectoderm (TE; Iqbal et al 2014), to chorionic girdle (Brosnahan et al 2012, Read et al 2018a) and term chorioallantois (Robles et al 2018, Loux et al 2019a). The majority of these studies have focused on characterising tissues of mixed cellular composition emphasising the importance of interpreting these observations alongside our understanding of tissue composition (Fig.…”

Section: Introductionmentioning

confidence: 99%

Markers of equine placental differentiation: insights from gene expression studies

et al. 2022

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Development and the subsequent function of the fetal membranes of the equine placenta requires both complex and precise regulation of gene expression. Advancements in recent years in bioinformatic techniques have allowed more extensive analyses into gene expression than ever before. This review starts by combining publically available transcriptomic datasets obtained from a range of embryonic, placental and maternal tissues, with previous knowledge of equine placental development and physiology, to gain insights into key gene families relevant to placentation in the horse. Covering the whole of pregnancy, the review covers trophectoderm, yolk sac, chorionic girdle cells, allantoamnion, allantochorion. In particular, 182 predicted ‘early high impact’ genes were identified (>100 TPM and >100 fold-change) that distinguish between progenitor trophectoderm, chorionic girdle tissue and allantochorion. Furthermore, 77 genes were identified as enriched in placental tissues (placental TPM > 10, with minimal expression in 12 non-placental < 1 TPM), including excellent candidates for functional studies such as IGF1, apolipoproteins, VGLL1, GCM1, CDX2 and FABP4. One gene with a currently unknown function was only identified (miR-675) in chorionic girdle but no other placental or adult tissues. It is pertinent that future studies focus on single cell transcriptomic approaches in order to determine how these changes in gene expression relate to tissue composition and start to better define trophoblast subpopulations in the equine placenta. Future functional characterisation of these genes and pathways will also be key not only to understanding normal placental development and fetal health but also their potential role in pathologies of pregnancy.

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Placental function and structure at term is altered in broodmares fed with cereals from mid-gestation

Cited by 13 publications

References 43 publications

Effects of dietary arginine supplementation in pregnant mares on maternal metabolism, placental structure and function and foal growth

Effects of dietary arginine supplementation in pregnant mares on maternal metabolism, placental structure and function and foal growth

Environmental constraints and pathologies that modulate equine placental genes and development

Markers of equine placental differentiation: insights from gene expression studies

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