Placental claudin expression and its regulation by endogenous sex steroid hormones

Ahn, Changhwan; Yang, Hyun; Lee, Dong‐Oh; An, Beum‐Soo; Jeung, Eui‐Bae

doi:10.1016/j.steroids.2015.05.001

Cited by 19 publications

(15 citation statements)

References 29 publications

(33 reference statements)

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“…The genes contained within this pathway include multiple chemokine ligands, claudins, matrix metallopeptidases, actins, myosins, and integrins (see Table S2A). Given the established biological roles of these gene products in reproductive biology this further suggests a role for DNA methylation in the regulation of tissue remodeling in placental development and function.…”

Section: Discussionmentioning

confidence: 99%

High‐resolution analysis of the human placental DNA methylome in early gestation

et al. 2020

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Background/Objective We communicate high‐read‐depth bisulfite sequencing analysis of the chorionic villus (CV) DNA methylome from samples obtained between 11 and 13 weeks gestation and samples of gestationally age‐matched maternal blood cells (MBC). Methods This was achieved through solution‐phase targeted region capture (84 Mb) of bisulfite converted human DNA. Results We identified biphasic distribution of methylation in CV and MBC genomes. We found greater numbers of intermediate methylated sites (20%‐80% methylated) in CV and greater number of high methylation sites in MBC and investigated distributions of these in promoters, introns, exons, CpG islands, CpG islands shores, and enhancers. We identified differentially methylated sites distinguishing CV and MBC. These are less likely to occur in CpG islands (CGIs), particularly those that exist outside promoters, exons, and introns. We found that gene promoter and gene body methylation patterns are associated with mRNA transcriptional profiles in CV. Despite the relative hypomethylation of CV genomes, we found that these contain DM regions that are more likely to be hypermethylated in CV relative to MBC. Conclusions Our data provide novel insight into the structure and organization of the CV epigenome, which may inform future studies of placental biology and noninvasive prenatal phenotyping.

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Section: Discussionmentioning

confidence: 99%

High‐resolution analysis of the human placental DNA methylome in early gestation

et al. 2020

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“…In the middle of mice pregnancy, the placenta showed increased expression of claudins 1-7, claudin-11, claudin-12, and claudin-23 (especially claudin-1, -2, -4, and -5), indicating their role in paracellular transportation during exchange of, e.g., nutrients, ions between mother and fetus. Moreover, their expression was reported to be modulated by steroid hormones [42]. Amniotic fluid volume is regulated by absorption across the amniotic epithelial cells [43].…”

Section: Uterusmentioning

confidence: 99%

Claudins: New Players in Human Fertility and Reproductive System Cancers

Kozieł

Kowalska

Piastowska-Ciesielska

2020

Cancers

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Claudins are major integral proteins of tight junctions (TJs), the apical cell-cell adhesions that enable maintaining polarity of epithelial cells, their differentiation, and cell signaling. A number of studies have indicated that claudins might play a crucial role in both physiology and pathogenesis. Their tissue-specific expression was originally linked to the development of different types of cancer and triggered a hope to use them as diagnostic or prognostic markers. However, it seems that their expression is more complex than that, and undoubtedly, claudins participate in one of the most important molecular events in cells. This review summarizes the recent research evaluating the role of claudins in fertility and the most common endocrine-dependent cancers in the reproductive system and highlights the crucial role of claudins both in human fertility and the most common cancers.

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“…The influence of pregnancy on the integrity of TJs has primarily been studied in endothelial cells of the placenta ( Marzioni et al, 2001 ; Ahn et al, 2015 ), and to date, we are not aware of any studies reporting the effects of a healthy pregnancy on the expression of TJ proteins in the maternal BBB. During normal pregnancy, high plasma levels of vascular endothelial growth factor (VEGF), an angiogenic mediator that increases BBB permeability through changes in the expression of TJ proteins ( Lafuente et al, 2006 ) including claudin-5 and occludin ( Argaw et al, 2012 ), have been reported ( Evans et al, 1998 ).…”

Section: Expression and Functionality Of Blood-brain Barrier Tight Jumentioning

confidence: 99%

Drug Transport at the Brain and Endothelial Dysfunction in Preeclampsia: Implications and Perspectives

Torres-Vergara

Escudero²,

Penny

2018

Front. Physiol.

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Transport of drugs across biological barriers has been a subject of study for decades. The discovery and characterization of proteins that confer the barrier properties of endothelia and epithelia, including tight junction proteins and membrane transporters belonging to the ATP-binding cassette (ABC) and Solute Carrier (SLC) families, represented a significant step forward into understanding the mechanisms that govern drug disposition. Subsequently, numerous studies, including both pre-clinical approaches and clinical investigations, have been carried out to determine the influence of physiological and pathological states on drug disposition. Importantly, there has been increasing interest in gaining a better understanding of drug disposition during pregnancy, since epidemiological and clinical studies have demonstrated that the use of medications by pregnant women is significant and this condition embodies a series of significant anatomical and physiological modifications, particularly at excretory organs and barrier sites (e.g., placenta, breast) expressing transporter proteins which influence pharmacokinetics. Currently, most of the research in this field has focused on the expression profiling of transporter proteins in trophoblasts and endothelial cells of the placenta, regulation of drug-resistance mechanisms in disease states and pharmacokinetic studies. However, little attention has been placed on the influence that the cerebrovascular dysfunction present in pregnancy-related disorders, such as preeclampsia, might exert on drug disposition in the mother’s brain. This issue is particularly important since recent findings have demonstrated that preeclamptic women suffer from long-term alterations in the integrity of the blood-brain barrier (BBB). In this review we aim to analyze the available evidence regarding the influence of pregnancy on the expression of transporters and TJ proteins in brain endothelial cells, as well the mechanisms that govern the pathophysiological alterations in the BBB of women who experience preeclampsia. Future research efforts should be focused not only on achieving a better understanding of the influence of preeclampsia-associated endothelial dysfunction on drug disposition, but also in optimizing the pharmacological treatments of women suffering pregnancy-related disorders, its comorbidities and to develop new therapies aiming to restore the integrity of the BBB.

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Placental claudin expression and its regulation by endogenous sex steroid hormones

Cited by 19 publications

References 29 publications

High‐resolution analysis of the human placental DNA methylome in early gestation

High‐resolution analysis of the human placental DNA methylome in early gestation

Claudins: New Players in Human Fertility and Reproductive System Cancers

Drug Transport at the Brain and Endothelial Dysfunction in Preeclampsia: Implications and Perspectives

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