Placebo-controlled pilot study of the effects of an eggshell membrane-based supplement on mobility and serum biomarkers in dogs with osteoarthritis

Muller, C.; Enomoto, Masataka; Buono, Angelo Del; Steiner, Jörg M.; Lascelles, B. Duncan X.

doi:10.1016/j.tvjl.2019.105379

Cited by 17 publications

(23 citation statements)

References 37 publications

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“…Serum triglycerides were measured at UCD Veterinary Clinical Laboratory Services. Plasma cytokines (IL-2, IL-6, IL-8, and TNF-α) were measured using a commercial multiplex electrochemiluminescence immunoassay (Meso Scale Diagnostics), as previously described 20 . Lipopolysaccharide (LPS) endotoxin was measured in plasma by Limulus Amoebocyte Lysate Pyrogent-5000 from Lonza (catalog #N384).…”

Section: Methodsmentioning

confidence: 99%

Fenofibrate promotes PPARα-targeted recovery of the intestinal epithelial barrier at the host-microbe interface in dogs with diabetes mellitus

Crakes

Pires

Quach

et al. 2021

Sci Rep

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Diabetes mellitus (DM) is associated with a dysfunctional intestinal barrier and an increased risk for systemic infection and inflammation in people, though the pathogenic mechanisms leading to this are poorly understood. Using a canine model of DM, we showed that the peroxisomal proliferator-activated receptor-α agonist fenofibrate modulates plasma lipid profiles and markers of intestinal barrier function. A 3-week course of fenofibrate reduced fasting interstitial glucose and inflammatory cytokine IL-8 and TNF-α concentrations, which correlated with reduced triglyceride levels. The lipidomic profile exhibited significantly lower levels of triacylglycerols, phosphatidylethanolamines, diacylglycerols, and ceramides following fenofibrate administration. On histopathological analysis, we observed an aberrant amount of intraepithelial CD3+ T lymphocytes (IEL) in the small intestine of dogs with spontaneous and induced-DM. Fenofibrate reduced IEL density in the duodenum of dogs with DM and enhanced markers of intestinal barrier function in vivo and in vitro. There were minimal changes in the intestinal microbial composition following fenofibrate administration, suggesting that repair of intestinal barriers can be achieved independently of the resident microbiota. Our findings indicate that lipid metabolism is critical to functionality of the intestinal epithelium, which can be rescued by PPARα activation in dogs with DM.

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Section: Methodsmentioning

confidence: 99%

Fenofibrate promotes PPARα-targeted recovery of the intestinal epithelial barrier at the host-microbe interface in dogs with diabetes mellitus

Crakes

Pires

Quach

et al. 2021

Sci Rep

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“…The serum and synovial fluid samples used in the work described here were collected from client-owned pet dogs that had been carefully evaluated and found to have OA-associated pain or found to be normal (i.e., pain-phenotyped animals), as a result of their involvement in veterinary clinical research studies (Muller et al, 2019) or ongoing translational pain research studies at NC State College of Veterinary Medicine. The breeds of dogs included were representative of the medium to large breed dogs that present for OA-associated pain.…”

Section: Collection Of Tissues and Fluidsmentioning

confidence: 99%

“…*OA dogs had multiple joints affected by OA and pain. Serum and synovial fluid samples were procured from different cohorts of dogs: a Samples taken from pet dogs at screening prior to clinical study (Muller et al, 2019); b Samples taken from pet dogs in pilot study prior to intra-articular injection of an experimental therapeutic (unpublished); c Samples taken at euthanasia of research dogs with unilateral OA.…”

Section: Collection Of Tissues and Fluidsmentioning

confidence: 99%

“…Having found increased expression of GFRα3, we investigated whether circulating or local levels of artemin were increased in dogs with painful OA. Serum samples were collected from non-OA healthy controls and dogs with OA-associated pain at the time of screening, prior to their involvement in a clinical study (Muller et al, 2019). The demographics of the dogs are tabulated in Table 1 (columns 1 and 2).…”

Section: Upregulation Of Serum Artemin In Osteoarthritic Dogsmentioning

confidence: 99%

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Correlation of Artemin and GFRα3 With Osteoarthritis Pain: Early Evidence From Naturally Occurring Osteoarthritis-Associated Chronic Pain in Dogs

et al. 2020

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Arthritis, including osteoarthritis (OA) and other musculoskeletal-associated pain, is a worldwide problem, however, effective drug options are limited. Several receptors, neurotransmitters, and endogenous mediators have been identified in rodent models, but the relevance of these molecules in disease-associated pain is not always clear. Artemin, a neurotrophic factor, and its receptor, glial-derived neurotrophic factor (GDNF) family receptor alpha-3 (GFRα3), have been identified as involved in pain in rodents. Their role in OA-associated pain is unknown. To explore a possible association, we analyzed tissue from naturally occurring OA in dogs to characterize the correlation with chronic pain. We used behavioral assessment, objective measures of limb use, and molecular tools to identify whether artemin and GFRα3 might be associated with OA pain. Our results using banked tissue from well-phenotyped dogs indicates that artemin/GFRα3 may play an important, and hitherto unrecognized, role in chronic OA-associated pain. Elevated serum levels of artemin from osteoarthritic humans compared to healthy individuals suggest translational relevance. Our data provide compelling evidence that the artemin/GFRα3 signaling pathway may be important in OA pain in both non-humans and humans and may ultimately lead to novel therapeutics.

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“…The use of long-term treatments with traditional drugs is the elective choice among clinicians, but most of times [ 25 ] it could lead to a high risk of developing side effects in patients. An alternative approach is to use natural products for treating OA in both humans and animals with different results as reported in literature [ 26 – 28 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of a dietary supplement in dogs with osteoarthritis: A randomized placebo-controlled, double-blind clinical trial

Martello

Bigliati

Adami³

et al. 2022

PLoS ONE

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This study is a randomized, placebo-controlled, double-blinded trial performed to investigate the effects of a dietary supplement containing a mixture of Boswellia serrata Roxb., chlorophyll, green tea extract, glucosamine, chondroitin sulfate, hyaluronic acid, and further in the manuscript: non-hydrolised type II collagen in dogs with osteoarthritis (OA). A total of 40 dogs were enrolled in the study, they were randomly divided in control (CTR) and treatment (TRT) groups. The TRT group received the dietary supplement for 60 days. The CTR group received a placebo for the same number of days. All the subjects had veterinary evaluations during the trial and owners were requested to fill in questionnaires on chronic pain using the Helsinki Chronic Pain Index. The product was easy to administer and no side effects were reported. Combining results from veterinarian and owner evaluations, the tested product proved to be significantly beneficial in alleviating pain and in reducing the clinical signs in dogs with OA.

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Placebo-controlled pilot study of the effects of an eggshell membrane-based supplement on mobility and serum biomarkers in dogs with osteoarthritis

Cited by 17 publications

References 37 publications

Fenofibrate promotes PPARα-targeted recovery of the intestinal epithelial barrier at the host-microbe interface in dogs with diabetes mellitus

Fenofibrate promotes PPARα-targeted recovery of the intestinal epithelial barrier at the host-microbe interface in dogs with diabetes mellitus

Correlation of Artemin and GFRα3 With Osteoarthritis Pain: Early Evidence From Naturally Occurring Osteoarthritis-Associated Chronic Pain in Dogs

Efficacy of a dietary supplement in dogs with osteoarthritis: A randomized placebo-controlled, double-blind clinical trial

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