The present study was carried out, to assess the knowledge of tribal females of Kargil, about menstruation. The sample for the study consisted of 320 adolescent's girls, 100 mothers and 100 grandmothers, belonging to Balti and Purig tribes. The random sampling technique was used, to select the sample. A self devised interview schedule was used, to gather the required information. The results reveal that, there is a substantial lacuna in the knowledge about menstruation, among the females. Widespread misconceptions and unawareness about the essential concepts of reproductive physiology and menstruation, among the tribal females was noted. It was found that, all mothers and the majority of grandmothers and adolescent girls, held incorrect perception, regarding the causes of menstruation. For them, menstruation occurs in females, in order to get rid of spoiled or dirty blood, from the body and it helps to detoxify our body. Many of these females were still not sure, about the source of menstrual blood. These girls were not able to differentiate between urethra and vaginal opening. The results overall highlight that, even today females of Kargil, tend to have insufficient and incomplete information, related to menarche and menstruation.