PL-4 (CIP596131.4): an Improved Potato Haploid Inducer

Abstract

Dihaploid production from elite tetraploid cultivars is key to both traditional and novel breeding approaches that seek to simplify potato genetics. For this purpose, efficient and widely compatible haploid inducers (HIs) are needed. We compared PL-4, a new HI developed at the International Potato Center, to known HIs IvP101 and IvP35. By pollination of elite tetraploid breeding lines, we showed that PL-4 performed significantly better and had a homogeneous response regardless of the genetic background of the pistillate parents, on the most important efficiency traits—number of dihaploids per 100 fruits and haploid induction rate. Moreover, PL-4 exhibited a reduced proportion of hybrid seeds, a convenient trait for efficient screening. In this context, we recommend PL-4 as an enhanced HI for the potato breeding community.

A simple and efficient Agrobacterium tumefaciens mediated transgenic system for tetraploid potato cultivar Desiree

“…The isolated plant nuclear groups had characteristic uorescence emission peaks. The DNA content of plantlets regenerated by diploid receptors were compared to potato material PL4 42 , which worked as diploid control, the lowest peak corresponding to 2C nuclei, and the remaining peaks representing 4C and 8C nuclei, respectively. And the plantlets regenerated from Desiree were compared to their recipient, the lowest peak corresponding to 4C nuclei.…”

Potato and sweetpotato breeding at the International Potato Center: approaches, outcomes and the way forward

“…CIP has taken steps to contribute to this possible transition of potato breeding. Firstly, a new germplasm source for the induction of haploids from tetraploid potato that combines advantages of the well-known IVP stocks (Hermsen and Verdenius 1973 ; Hutten et al 1994 ) was recently released (Ordoñez et al 2021 ). Secondly, selected elite tetraploid clones were pollinated with three haploid inducers (HI) (IVP-35, IVP-101, and the above-mentioned PL-4 to initiate conversion of advanced tropically adapted populations to sets of diploid inbred parental lines.…”

Unlikely heroes on the long and winding road to potato inbreeding

“…A solution could be an efficient haploid induction system, which generates inbreds in one or two generations (Jacquier et al 2020 ). Currently, potato haploid inducers are used for 4× to 2× conversion and the efficiency varies from 1 to 0.001 per pollination (Amundson et al 2021 ; Ordoñez et al 2021 ; Busse et al 2021 ; Hermsen and Verdenius 1973 ). If a breeding program could generate thousands of 1 × haploids from 2 × parents, selection for vigor could start with their germination, which requires little space and effort.…”