PKS5, a SNF1-related kinase, interacts with and phosphorylates NPR1, and modulates expression of WRKY38 and WRKY62

Xie, Changgen; Zhou, Xiaona; Deng, Xing‐Wang; Guo, Yan

doi:10.1016/s1673-8527(09)60054-0

Cited by 50 publications

(40 citation statements)

References 32 publications

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“…Our results and other recent reports that suggest the participation of rice CIPK14/15 in regulation of defense gene expression and phytoalexin production in rice cultured cells and the possible regulation of NPR1 by PKS5 (Arabidopsis CIPK24) phosphorylation (Kurusu et al, 2010;Xie et al, 2010) support a role for CBL/CIPK participation in different aspects of plant defense. Thus, CBL/CIPK complexes are important players in modulating outputs during biotic interactions.…”

Section: Discussionsupporting

confidence: 87%

The Tomato Calcium Sensor Cbl10 and Its Interacting Protein Kinase Cipk6 Define a Signaling Pathway in Plant Immunity

et al. 2013

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Section: Discussionsupporting

confidence: 87%

The Tomato Calcium Sensor Cbl10 and Its Interacting Protein Kinase Cipk6 Define a Signaling Pathway in Plant Immunity

et al. 2013

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“…Two Superactive PKS5 Mutants Are Hypersensitive to ABA To determine if the loss or gain of function of PKS5 leads to altered sensitivity to ABA, we examined the response of previously characterized pks5 mutants (Fuglsang et al, 2007;Xie et al, 2010;Yang et al, 2010) to ABA during seed germination. We found that two hyperactive kinase mutants of PKS5 (Yang et al, 2010), pks5-3 (Ser-317 replaced by Leu) and pks5-4 (Ala-168 replaced by Val), with amino acid changes in either the FISL/NAF (a conserved 21-amino acid peptide that is necessary for interaction with SOS3 or SOS3-LIKE CALCIUM BINDING PROTEINs) motif or in the kinase activation loop (Guo et al, 2001), were hypersensitive to ABA (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To introduce the S42A, S145A, S182A, and T201A mutations into ABI5, we performed two-round-PCR-based nucleotide replacement as described previously (Quan et al, 2007;Xie et al, 2010). The primers used to construct the plasmids are listed in Supplemental Table S1.…”

Section: Plasmid Constructionmentioning

confidence: 99%

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SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE5

et al. 2015

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Abscisic acid (ABA) plays an essential role in seed germination. In this study, we demonstrate that one SNF1-RELATED PROTEIN KINASE3-type protein kinase, SOS2-LIKE PROTEIN KINASE5 (PKS5), is involved in ABA signal transduction via the phosphorylation of an interacting protein, ABSCISIC ACID-INSENSITIVE5 (ABI5). We found that pks5-3 and pks5-4, two previously identified PKS5 superactive kinase mutants with point mutations in the PKS5 FISL/NAF (a conserved peptide that is necessary for interaction with SOS3 or SOS3-LIKE CALCIUM BINDING PROTEINs) motif and the kinase domain, respectively, are hypersensitive to ABA during seed germination. PKS5 was found to interact with ABI5 in yeast (Saccharomyces cerevisiae), and this interaction was further confirmed in planta using bimolecular fluorescence complementation. Genetic studies revealed that ABI5 is epistatic to PKS5. PKS5 phosphorylates a serine (Ser) residue at position 42 in ABI5 and regulates ABA-responsive gene expression. This phosphorylation was induced by ABA in vivo and transactivated ABI5. Expression of ABI5, in which Ser-42 was mutated to alanine, could not fully rescue the ABA-insensitive phenotypes of the abi5-8 and pks5-4abi5-8 mutants. In contrast, mutating Ser-42 to aspartate rescued the ABA insensitivity of these mutants. These data demonstrate that PKS5-mediated phosphorylation of ABI5 at Ser-42 is critical for the ABA regulation of seed germination and gene expression in Arabidopsis (Arabidopsis thaliana).

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“…Intriguingly, this group of genes includes three protein phosphorylation enzymes (two kinases and a phosphatase) with known positive effects on defense or defense gene expression: PKS5, WIN2, and CRK45. PKS5 phosphorylates NPR1, and expression of two NPR1 target genes, WRKY38 and WRKY62, is reduced in pks5 mutants (Xie et al, 2010). Overexpression of the phosphatase WIN2 enhances resistance against DC3000, and WIN2 has been shown to interact with HopW1-1, an effector present in DC3000 (Lee et al, 2008).…”

Section: Multiple Time Series Coexpression Analysis Predicts Specificmentioning

confidence: 99%

Transcriptional Dynamics Driving MAMP-Triggered Immunity and Pathogen Effector-Mediated Immunosuppression in Arabidopsis Leaves Following Infection with Pseudomonas syringae pv tomato DC3000

et al. 2015

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Transcriptional reprogramming is integral to effective plant defense. Pathogen effectors act transcriptionally and posttranscriptionally to suppress defense responses. A major challenge to understanding disease and defense responses is discriminating between transcriptional reprogramming associated with microbial-associated molecular pattern (MAMP)-triggered immunity (MTI) and that orchestrated by effectors. A high-resolution time course of genome-wide expression changes following challenge with Pseudomonas syringae pv tomato DC3000 and the nonpathogenic mutant strain DC3000hrpA-allowed us to establish causal links between the activities of pathogen effectors and suppression of MTI and infer with high confidence a range of processes specifically targeted by effectors. Analysis of this information-rich data set with a range of computational tools provided insights into the earliest transcriptional events triggered by effector delivery, regulatory mechanisms recruited, and biological processes targeted. We show that the majority of genes contributing to disease or defense are induced within 6 h postinfection, significantly before pathogen multiplication. Suppression of chloroplast-associated genes is a rapid MAMP-triggered defense response, and suppression of genes involved in chromatin assembly and induction of ubiquitin-related genes coincide with pathogen-induced abscisic acid accumulation. Specific combinations of promoter motifs are engaged in fine-tuning the MTI response and active transcriptional suppression at specific promoter configurations by P. syringae.

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PKS5, a SNF1-related kinase, interacts with and phosphorylates NPR1, and modulates expression of WRKY38 and WRKY62

Cited by 50 publications

References 32 publications

The Tomato Calcium Sensor Cbl10 and Its Interacting Protein Kinase Cipk6 Define a Signaling Pathway in Plant Immunity

The Tomato Calcium Sensor Cbl10 and Its Interacting Protein Kinase Cipk6 Define a Signaling Pathway in Plant Immunity

SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE5

Transcriptional Dynamics Driving MAMP-Triggered Immunity and Pathogen Effector-Mediated Immunosuppression in Arabidopsis Leaves Following Infection with Pseudomonas syringae pv tomato DC3000

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