pKa-Tunable fluorescent probes for visualizing minor pH fluctuations in different subcellular organelles and in vivo

Li, Lulu; He, Dan; Chen, Xiao; Yang, Sanxiu; Yang, Yunfei; Gong, Aihua; Wang, Kun; Qian, Jing; Long, Lingliang

doi:10.1016/j.dyepig.2023.111608

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…To on-site quantitatively measure sulfite in food, fluorescent sensors need to have the following characteristics: (1) The fluorescent sensors preferably respond to sulfite in the near-infrared (NIR) fluorescence range (650-900 nm), as the fluorescence in this range might reduce the interference of autofluorescence in food samples [33][34][35]. (2) The fluorescent sensors should exhibit a ratiometric fluorescence response to sulfite as it could effectively eliminate the fluorescence intensity errors originating from sensor distribution, excitation light intensity, photobleaching [36][37][38], etc. (3) Fluorescent sensors are better for displaying colorimetric responses to sulfite, because both fluorescence and colorimetric dual-signal responses to sulfite will make the measurement more reliable [39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Construction of a Colorimetric and Near-Infrared Ratiometric Fluorescent Sensor and Portable Sensing System for On-Site Quantitative Measurement of Sulfite in Food

Chen,

Zhao,

Zhao

et al. 2024

Foods

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Sulfites play imperative roles in food crops and food products, serving as sulfur nutrients for food crops and as food additives in various foods. It is necessary to develop an effective method for the on-site quantification of sulfites in food samples. Here, 7-(diethylamino) quinoline is used as a fluorescent group and electron donor, alongside the pyridinium salt group as an electron acceptor and the C=C bond as the sulfite-specific recognition group. We present a novel fluorescent sensor based on a mechanism that modulates the efficiency of intramolecular charge transfer (ICT), CY, for on-site quantitative measurement of sulfite in food. The fluorescent sensor itself exhibited fluorescence in the near-infrared light (NIR) region, effectively minimizing the interference of background fluorescence in food samples. Upon exposure to sulfite, the sensor CY displayed a ratiometric fluorescence response (I447/I692) with a high sensitivity (LOD = 0.061 μM), enabling accurate quantitative measurements in complex food environments. Moreover, sensor CY also displayed a colorimetric response to sulfite, making sensor CY measure sulfite in both fluorescence and colorimetric dual-signal modes. Sensor CY has been utilized for quantitatively measuring sulfite in red wine and sugar with recoveries between 99.65% and 101.90%, and the RSD was below 4.0%. The sulfite concentrations in live cells and zebrafish were also monitored via fluorescence imaging. Moreover, the sulfite assimilated by lettuce leaves was monitored, and the results demonstrated that excessive sulfite in leaf tissue could lead to leaf tissue damage. In addition, the sulfate-transformed sulfite in lettuce stem tissue was tracked, providing valuable insights for evaluating sulfur nutrients in food crops. More importantly, to accomplish the on-site quantitative measurement of sulfite in food samples, a portable sensing system was prepared. Sensor CY and the portable sensing system were successfully used for the on-site quantitative measurement of sulfite in food.

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Section: Introductionmentioning

confidence: 99%

Construction of a Colorimetric and Near-Infrared Ratiometric Fluorescent Sensor and Portable Sensing System for On-Site Quantitative Measurement of Sulfite in Food

Chen,

Zhao,

Zhao

et al. 2024

Foods

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pH‐Responsive Aminobenzocoumarins as Fluorescent Probes for Biological Acidity

Schniererová,

Janeková,

Joniak

et al. 2024

Chemistry A European J

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Regulation of pH plays an essential role in orchestrating the delicate cellular machinery responsible for life as we know it. Its abnormal values are indicative of aberrant cellular behavior and associated with pathologies including cancer progression or solid tumors. Here, we report a series of bent and linear aminobenzocoumarins decorated with different substituents. We investigate their photophysical properties and demonstrate that the probes display strong pH‐responsive fluorescence “turn on” behavior in highly acidic environments, with enhancement up to 300‐fold. In combination with their low cytotoxicity, this behavior enabled their application in bioimaging of acidic lysosomes in live human cells. We believe that these molecules serve as attractive lead structures for future rational design of novel biocompatible fluorescent pH probes.

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A coumarin-naphthalimide-based ratiometric fluorescent probe for nitroxyl (HNO) based on an ICT-FRET mechanism

Ma,

Liu,

et al. 2024

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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pKa-Tunable fluorescent probes for visualizing minor pH fluctuations in different subcellular organelles and in vivo

Cited by 3 publications

References 37 publications

Construction of a Colorimetric and Near-Infrared Ratiometric Fluorescent Sensor and Portable Sensing System for On-Site Quantitative Measurement of Sulfite in Food

Construction of a Colorimetric and Near-Infrared Ratiometric Fluorescent Sensor and Portable Sensing System for On-Site Quantitative Measurement of Sulfite in Food

pH‐Responsive Aminobenzocoumarins as Fluorescent Probes for Biological Acidity

A coumarin-naphthalimide-based ratiometric fluorescent probe for nitroxyl (HNO) based on an ICT-FRET mechanism

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