The P-type ATPase CrpA is an important Cu 2+ /Cd 2+ pump in the Aspergilli, significantly contributing to the heavy metal stress tolerance of these ascomycetous fungi. As expected, the deletion of crpA resulted in Cu 2+ /Cd 2+ -sensitive phenotypes in Aspergillus nidulans on stress agar plates inoculated with conidia.Nevertheless, paradoxical growth stimulations were observed with the ΔcrpA strain in both standard Cu 2+ stress agar plate experiments and cellophane colony harvest (CCH) cultures, when exposed to Cd 2+ . These observations reflect efficient compensatory mechanisms for the loss of CrpA operating under these experimental conditions. It is remarkable that the ΔcrpA strain showed a 2.7 times higher Cd biosorption capacity in CCH cultures, which may facilitate the development of new, fungal biomass-based bioremediation technologies to extract harmful Cd 2+ ions from the environment. The nullification of crpA also significantly changed the spatial distribution of Cu and Cd in CCH cultures, as