PiVR: an affordable and versatile closed-loop platform to study unrestrained sensorimotor behavior

Tadres, David; Louis, Matthieu

doi:10.1101/2019.12.20.885442

Cited by 12 publications

(21 citation statements)

References 70 publications

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“…These results reproduce the known effects of natural sugar or bitter (Flood et al, 2013; Thoma et al, 2016; Marella et al, 2006; Joseph and Heberlein, 2012; Das et al, 2014; Schwaerzel et al, 2003), providing reassurance that our activation paradigms are relevant to natural behavior. Our results also align with other studies that reported effects on locomotion or innate preference using optogenetic taste neuron activation (Aso et al, 2014; Tadres and Louis, 2020; Mohammad et al, 2017; Haberkern et al, 2019; DeAngelis et al, 2020; Stern and Yang, 2017).…”

Section: Discussionsupporting

confidence: 92%

Selective integration of diverse taste inputs within a single taste modality

Deere

Uttley

Santana

et al. 2022

Preprint

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Each sensory system contains a diverse repertoire of sensory cells. In the gustatory system, individual taste modalities contain sensory cells that reside in different locations or express different combinations of taste receptors. These different input channels may converge onto common downstream circuits and drive the same behaviors, or they may activate separate pathways to regulate distinct behaviors. To distinguish between these models in Drosophila, we first characterized a repertoire of behaviors induced by optogenetic activation of sugar- or bitter-sensing neurons. We then activated subsets of bitter-sensing neurons within specific organs. Different subsets elicited a broad and highly overlapping set of behaviors, suggesting that they converge onto common downstream pathways, but we also observed behavioral differences that argue for selective convergence. Transsynaptic tracing experiments provided a neuronal substrate for these results, revealing that bitter neuron subsets connect to overlapping downstream regions with biased connectivity. We characterized one type of second-order bitter neuron that integrates multiple types of bitter input and regulates a subset of bitter-induced behaviors. These results suggest that different bitter inputs are selectively integrated early in the circuit, enabling the pooling of sensory information, and the circuit then diverges into multiple pathways that may have different behavioral roles.

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Section: Discussionsupporting

confidence: 92%

Selective integration of diverse taste inputs within a single taste modality

Deere

Uttley

Santana

et al. 2022

Preprint

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“…(2020) present a portable flow‐through microscope to study planktonic communities that can be used in remote field locations. Tadres and Louis (2020) used the Raspberry Pi to create a low‐cost virtual reality platform (PiVR) that enables high‐resolution, optogenetic, closed‐loop experiments with small freely moving animals at up to 50 Hz. Kallmyer et al.…”

Section: Overview Of Applications Across the Biological Domainmentioning

confidence: 99%

Broad‐scale applications of the Raspberry Pi: A review and guide for biologists

Jolles

2021

Methods Ecol Evol

140

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1. The field of biology has seen tremendous technological progress in recent years, fuelled by the exponential growth in processing power and high-level computing, and the rise of global information sharing. Low-cost single-board computers are predicted to be one of the key technological advancements to further revolutionise this field.2. So far, an overview of current uptake of these devices and a general guide to help researchers integrate them in their work has been missing. In this paper I focus on the most widely used single-board computer, the Raspberry Pi, and review its broad applications and uses across the biological domain.3. Since its release in 2012, the Raspberry Pi has been increasingly taken up by biologists, in the laboratory, the field and in the classroom, and across a wide range of disciplines. A hugely diverse range of applications exists that ranges from simple solutions to dedicated custom-build devices, including nest-box monitoring, wildlife camera trapping, high-throughput behavioural recording, large-scale plant phenotyping, underwater video surveillance, closed-loop operant learning experiments and autonomous ecosystem monitoring. Despite the breadth of its implementations, the depth of uptake of the Raspberry Pi by the scientific community is still limited. 4. The broad capabilities of the Raspberry Pi, combined with its low cost, ease of use and large user community make it a great research tool for almost any project. To help accelerate the uptake of the Raspberry Pi by the scientific community, I provide detailed guidelines, recommendations and considerations, and 30+ step-by-step guides on a dedicated accompanying website (http://raspb erryp i-guide.github.io). I hope this paper will help generate more awareness about the Raspberry Pi among scientists and thereby both fuel the democratisation of science and ultimately help advance our understanding of biology, from the micro-to the macro-scale.

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“…Closed-loop systems are powerful tools to identify various strategies of an animal's sensorimotor transformation. They have been employed in many animal models including adult and larval Drosophila (Bahl et al, 2013;Tadres and Louis, 2019), larval zebrafish (Chen and Engert, 2014;Bahl and Engert, 2020), and C. elegans (Leifer et al, 2011;Kocabas et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Navigational strategies underlying temporal phototaxis inDrosophilalarvae

Zhu

Herrera

Vogt

et al. 2020

Preprint

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statementUsing a novel closed-loop behavioral assay, we show that Drosophila larvae can navigate light gradients exclusively using temporal cues. Analyzing and modeling their behavior in detail, we propose that larvae achieve this by accumulating luminance change during runs. AbstractNavigating across light gradients is essential for survival for many animals. However, we still have a poor understanding of the algorithms that underlie such behaviors. Here we develop a novel phototaxis assay for Drosophila larvae in which light intensity is always spatially uniform but constantly updates depending on the location of the animal in the arena. Even though larvae can only rely on temporal cues in this setup, we find that they are capable of finding preferred areas of low light intensity. Further detailed analysis of their behavior reveals that larvae initiate turns more frequently and that turn amplitudes become higher when animals experience luminance increments over extended periods of time. We suggest that temporal integration of luminance change during runs is an important -and so far largely unexplored -element of phototaxis.

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PiVR: an affordable and versatile closed-loop platform to study unrestrained sensorimotor behavior

Cited by 12 publications

References 70 publications

Selective integration of diverse taste inputs within a single taste modality

Selective integration of diverse taste inputs within a single taste modality

Broad‐scale applications of the Raspberry Pi: A review and guide for biologists

Navigational strategies underlying temporal phototaxis inDrosophilalarvae

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