Pituitary tumor transforming gene binding factor (PBF) is required for androgen-induced prostate cancer proliferation and invasion

Huang, Shu‐Zhen; Wang, B; Liao, Qianjin; Shen, Cha; Li, W B

doi:10.4149/neo_2018_180730n552

Cited by 2 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that all four proteins have important roles in tumor growth and metastasis in various types of cancer. [ 47,53–66 ] The collective low levels of these proteins that are involved in the intrinsic and extrinsic apoptosis signaling pathways in cell line X2 highlight their interconnected roles in apoptosis regulation in CHO cells. Understanding their interplay and functional associations can provide valuable insights for developing novel apoptotic‐resistant CHO cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that all four proteins have important roles in tumor growth and metastasis in various types of cancer. [47,[53][54][55][56][57][58][59][60][61][62][63][64][65][66] The collective low levels of these proteins that are involved in the intrinsic and extrinsic apoptosis signaling pathways in cell line X2 highlight their interconnected roles in apoptosis regulation in CHO cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells

Martínez,

Rodriguez,

McCubbin

et al. 2024

Biotechnology Journal

View full text Add to dashboard Cite

Chinese hamster ovary (CHO) cells are widely used to produce complex biopharmaceuticals. Improving their productivity is necessary to fulfill the growing demand for such products. One way to enhance productivity is by cultivating cells at high densities, but inhibitory by‐products, such as metabolite derivatives from amino acid degradation, can hinder achieving high cell densities. This research examines the impact of these inhibitory by‐products on high‐density cultures. We cultured X1 and X2 CHO cell lines in a small‐scale semi‐perfusion system and introduced a mix of inhibitory by‐products on day 10. The X1 and X2 cell lines were chosen for their varied responses to the by‐products; X2 was susceptible, while X1 survived. Proteomics revealed that the X2 cell line presented changes in the proteins linked to apoptosis regulation, cell building block synthesis, cell growth, DNA repair, and energy metabolism. We later used the AB cell line, an apoptosis‐resistant cell line, to validate the results. AB behaved similar to X1 under stress. We confirmed the activation of apoptosis in X2 using a caspase assay. This research provides insights into the mechanisms of cell death triggered by inhibitory by‐products and can guide the optimization of CHO cell culture for biopharmaceutical manufacturing.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells

Martínez,

Rodriguez,

McCubbin

et al. 2024

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

IRS1 promotes thyroid cancer metastasis through EMT and PI3K/AKT pathways

Yu,

Huang,

Kuang

et al. 2024

Clinical Endocrinology

View full text Add to dashboard Cite

ObjectiveInsulin receptor substract 1 (IRS1) protein is an important signal transduction adapter for extracellular signal transduction from insulin‐like growth factor‐1 receptor and its family members to IRS1 downstream proteins. IRS1 has been reported to be involved in tumourigenesis and metastasis in some of solid tumors. Investigating the role of IRS1 in thyroid cancer can help to screen high risk patients at the initial diagnosis.Design, Patients and MeasurementsImmunohistochemical assay was used to detect the expression levels of IRS1 in 131 metastatic thyroid cancer tissues. Wound healing, cell invasion and colony formation assays were used to study the functions of IRS1 in vitro. RNA sequencing (RNA‐seq) and Western blot analysis analyses were performed to examine the underlying regulation mechanisms of IRS1 in thyroid cancer cells.ResultsIRS1 was highly expressed in thyroid cancers and its expression was positively associated with distant metastasis and advanced clinical stages. In vitro studies demonstrated that IRS1 is an important mediator of migration, invasion and colony formation of thyroid cancer cells. RNA‐seq showed that IRS1 promoted the metastasis of thyroid cancer by regulating epithelial‐mesenchymal transition and phosphoinositide 3‐kinase (PI3K)/AKT pathway.ConclusionsIRS1 overexpression contributes to the aggressiveness of thyroid cancer and is expected to be a stratified marker and a potential therapeutic target for thyroid cancer.

show abstract

Pituitary tumor transforming gene binding factor (PBF) is required for androgen-induced prostate cancer proliferation and invasion

Cited by 2 publications

References 26 publications

Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells

Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells

IRS1 promotes thyroid cancer metastasis through EMT and PI3K/AKT pathways

Contact Info

Product

Resources

About