Pituitary Pars Intermedia of Male Viscacha&lt;i&gt; (Lagostomus maximus maximus)&lt;/i&gt;: A Morphometric Study of Seasonal and Age-Related Changes in Immunohistochemistry

Acosta, Mariano; Mohamed, Fabián

doi:10.1159/000204751

Cited by 9 publications

(11 citation statements)

References 111 publications

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“…FSC expressed the S-100 protein and occasionally GFAP and vimentin, which are considered as markers of glial, astrocyte and immature glial cells, respectively. These results in PD support our previous hypothesis for viscacha PI 31 that FSC have a neuroectodermic origin. Moreover, the differential expression of the tested proteins and the immunostaining patterns suggest the presence of FSC in different functional stages, as has also been proposed for viscacha PI 31 and for other species.…”

Section: Discussionsupporting

confidence: 91%

“…In this pituitary zone, scarce vascularization and large follicles (major colloidal diameter: 39.87±0.49 µm) have been informed. 31 On the other hand, Mohamed et al 32 have described in pituitary PD follicles of smaller size (major colloidal diameter: 12.67±0.38 µm). In the present work, FSC of PD were pleomorphic and showed short cytoplasmic processes which contacted endocrine cells and blood vessels.…”

Section: Discussionmentioning

confidence: 98%

“…27, 31,33–36 The details, suppliers, dilution, time and temperature of the incubation of antibodies used are reported in Table 1. The tissue sections were first deparaffinized with xylene and hydrated by decreasing concentrations of ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…In this pituitary zone, the FSC originated follicles with PAS-positive colloid inside, expressed S-100, GFAP and vimentin, and were closely associated with melanotrophs. 31 Moreover, an ultrastructural study of the pituitary PD has demonstrated the existence of two types of colloid-containing structures: 1) follicles, originated by the FSC, and 2) colloid extracellular accumulations, limited by different types of granulated cells. 32 The follicular lumen was filled with abundant electrodense colloid and several cellular debris comprising vacuoles, altered membranes, mitochondria and secretory granules, which are probably a product of FSC phagocytotic activity.…”

Section: Introductionmentioning

confidence: 99%

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Folliculostellate cells in pituitary pars distalis of male viscacha: immunohistochemical, morphometric and ultrastructural study

Acosta¹,

Filippa²,

Mohamed³

2010

Eur J Histochem

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Folliculostellate cells (FSC) have been reported in pituitary of several mammalian species. FSC morphology and secreted substances have been instrumental to the understanding of their function. The purpose of this work was to perform an immunohistochemical, morphometric and ultrastructural study of the pituitary pars distalis FSC in adult male viscacha and to analyze their relation with hormone secreting cells. Immunohistochemistry and image analysis were carried out in different sectors of the gland, from the middle (sector 1) to the glandular periphery (sector 5). Transmission electron microscopy with lanthanum as electrodense tracer was used. FSC formed follicles with PAS-positive colloid inside. They expressed S-100 protein mainly in both nucleus and cytoplasm. FSC were stellate-like in shape and exhibited short cytoplasmic processes that contacted with blood vessels and endocrine cells. In addition, some follicular colloids were immunostained with anti-S-100 protein. A few FSC were immunostained with anti-glial fibrillary acidic protein (GFAP) and anti-vimentin. The morphometric parameters analyzed (percentages of S-100-positive total, cellular and colloidal areas) increased from sector 1 to sector 3 and then decreased to sector 5. Hormone secreting cells, mainly lactotrophs, gonadotrophs and corticotrophs were associated with FSC and follicles. The ultrastructural study demonstrated that FSC developed junctional complexes and desmosomes between their lateral membranes. Lanthanum freely penetrated the spaces between granulated cells and FSC, but did not penetrate into the follicular lumen. In conclusion: 1) the differential expression of S-100 protein, GFAP and vimentin may indicate different physiological stages of FSC; 2) the expression of these proteins suggests a neuroectodermic origin of these cells; 3) FSC spatial distribution, association with endocrine cells, and the generation of an intercellular communication network suggest that FSC are involved in the pituitary pars distalis paracrine regulation of the viscacha.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Folliculostellate cells in pituitary pars distalis of male viscacha: immunohistochemical, morphometric and ultrastructural study

Acosta¹,

Filippa²,

Mohamed³

2010

Eur J Histochem

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“…In addition to the secretory cells, the folliculostellate cells (FSC) have been reported in the pituitary of several mammalian species (Allaerts and Vankelecom 2005;Acosta and Mohamed 2009). They are star-shaped and follicle-forming cells in the anterior pituitary gland (Devnath and Inoue 2008) and are known to work as scavenger cells (Drewett et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Cadmium exposure modifies lactotrophs activity associated to genomic and morphological changes in rat pituitary anterior lobe

et al. 2009

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Cadmium (Cd) is widely used in industrial applications and is an important contaminant of agricultural products. As an endocrine disruptor, Cd modifies the hormone release of pituitary anterior lobe (PAL). This work was undertaken to evaluate a possible association between phospholipase D (PLD) and prolactin mRNA expressions and the activity of lactotrophs and folliculostellate cells (FSC) in PAL of Cd exposed adult male Wistar rats (Cd, 0.133 mM per liter for 2 months). The PALs were submitted to immunohistochemical and morphometric analysis to determine the percentage of lactotrophs (PRL-ir) and FSC (S-100-ir). Cultured PAL cells were stained with Hoechst 33258 to determine the presence of alterations in nuclear morphology consistent with apoptosis. The expressions of PLD and prolactin mRNA were assessed by RT-PCR. Cd treated rats showed a decrease of PLD mRNA levels that can be associated to both high number of apoptotic cells and increase of S-100 protein expression in FSC. Cd decreased prolactin mRNA expression, number of lactotrophs and percentage of PRL-ir suggesting a low availability of prolactin to be secreted from PAL. Cd modifies the lactotrophs activity of pituitary gland through biochemical, genomic and morphological changes and contributes directly or indirectly to the levels of serum prolactin.

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Hypophysial angiogenesis decodes annual time and underlies physiological adaptation to seasonal changes in the environment

Tortonese

2022

J Exp Zool Pt A

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Adaptation to annual changes in the environment is controlled by hypophysial hormones. In temperate zones, photoperiod is the primary external cue that regulates annual biological cycles and is translated by the pattern of melatonin secretion acting primarily in the hypophysial pars tuberalis. Angiogenic mechanisms within this tissue contribute to decode the melatonin signal through alternative splicing of the vascular endothelial growth factor A (VEGF-A) gene in both the pars tuberalis and the capillary loops of the infundibulum. The resulting melatonin-evoked differential productions of VEGF-A isoforms will induce seasonal remodeling of the vascular connection between the hypothalamus and hypophysis, and act as paracrine messengers in the pars distalis to generate the required seasonal endocrine response. Specifically, the long melatonin signal in winter upregulates antiangiogenic VEGF-A isoforms, which will reduce the number of vascular loops and the density of VEGF receptors in endocrine and folliculo-stellate (FS) cells, inhibit prolactin secretion, and stimulate FSH. In contrast, the short melatonin signal in summer upregulates proangiogenic VEGF-A isoforms that will increase the number of vascular loops and the density of VEGF receptors in endocrine and FS cells, stimulate prolactin secretion, and suppress FSH. A similar system has been identified in long day seasonal breeders, revealing that this is a conserved mechanism of adaptation across species. Thus, an angiogenesis-based, intrahypophysial system for annual time measurement controls local microvascular plasticity and conveys the photoperiodic signal readout from the melatonin sensitive pars tuberalis to the endocrine cells of the pars distalis to regulate seasonal adaptation to the environment.

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Pituitary Pars Intermedia of Male Viscacha<i> (Lagostomus maximus maximus)</i>: A Morphometric Study of Seasonal and Age-Related Changes in Immunohistochemistry

Cited by 9 publications

References 111 publications

Folliculostellate cells in pituitary pars distalis of male viscacha: immunohistochemical, morphometric and ultrastructural study

Folliculostellate cells in pituitary pars distalis of male viscacha: immunohistochemical, morphometric and ultrastructural study

Cadmium exposure modifies lactotrophs activity associated to genomic and morphological changes in rat pituitary anterior lobe

Hypophysial angiogenesis decodes annual time and underlies physiological adaptation to seasonal changes in the environment

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