Pituitary estrogen receptor alpha is involved in luteinizing hormone pulsatility at mid-gestation in the South American plains vizcacha, Lagostomus maximus (Rodentia, Caviomorpha)

Proietto, Sofía; Yankelevich, Lorena; Villarreal, Federico Martín; Inserra, Pablo Ignacio Felipe; Charif, Santiago Elías; Schmidt, Alejandro Raúl; Cortasa, Santiago Andrés; Corso, María Clara; Giorgio, Noelia Paula Di; Lux‐Lantos, Victoria; Vitullo, Alfredo Daniel; Halperín, Julia; Dorfman, Verónica Berta

doi:10.1016/j.ygcen.2018.04.001

Cited by 11 publications

(15 citation statements)

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“…Almost a decade ago, our laboratory reliably established reactivation of hypothalamic GnRH and pituitary LH pulsatilities well into the gestation of vizcachas [25,26]. This feature is related to a conspicuous characteristic exhibited by the ovaries of L. maximus (Family Chinchillidae) and shared by other hystricomorphs as the African mole-rats (Family Bathyergidae), and Old-World porcupines (Family Hystricidae): the existence of luteinized unruptured follicles (i.e., accessory corpora lutea, aCL) [41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, early in pregnancy, the onset of placental calcification and thinning of the uterine segmental arteries as they move away from the cervix and toward the ovary strongly suggest that the vizcacha placenta is, at best, steroidogenically deficient [22][23][24]. As a result of the decrease in circulating progesterone represented in the "valley" of the biphasic curve around day 90 of gestation, and the consequent elimination of negative feedback on gonadotropin release, a new set of accessory CL (aCL) arises in the midgestation [21,[25][26][27]. It has been attributed precisely to these aCL the recovery of the progesterone levels necessary to adequately complete embryonic development and to carry the pregnancy to term [21].…”

Section: Introductionmentioning

confidence: 99%

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Achieving full-term pregnancy in the vizcacha relies on a reboot of luteal steroidogenesis in mid-gestation (Lagostomus maximus, Rodentia)

et al. 2022

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Reactivation of the hypothalamic-pituitary-ovarian (HPO) axis triggered by the decline in serum progesterone in mid-gestation is an uncommon trait that distinguishes the vizcacha from most mammals. Accessory corpora lutea (aCL) developed upon this event have been proposed as guarantors of the restoration of the progesterone levels necessary to mantain gestation. Therefore, the steroidogenic input of primary CL (pCL) vs aCL was evaluated before and after HPO axis-reactivation (BP and AP respectively) and in term pregnancy (TP). Nonpregnant-ovulated females (NP) were considered as the pCL-starting point group. In BP, the ovaries mainly showed pCL, whose LH receptor (LHR), StAR, 3β-HSD, 20α-HSD, and VEGF immunoexpressions were similar or lower than those of NP. In AP, luteal reactivity increased significantly compared to the previous stages, and the pool of aCL developed in this stage represented 20% of the ovarian structures, equaling the percentage of pCL. Both pCL and aCL luteal cells shared similar histological features consistent with secretory activity. Although pCL and aCL showed equivalent labeling intensity for the luteotropic markers, pCL were significantly larger than aCL. Towards TP, both showed structural disorganization and loss of secretory characteristics. No significant DNA fragmentation was detected in luteal cells throughout gestation. Our findings indicate that the LH surge derived from HPO axis-reactivation targets the pCL and boost luteal steroidogenesis and thus progesterone production. Because there are many LHR-expressing antral follicles in BP, they also respond to the LH stimuli and luteinize without extruding the oocyte. These aCL certainly contribute but it is the steroidogenic restart of the pCL that is the main force that restores progesterone levels, ensuring that gestation is carried to term. Most importantly, the results of this work propose luteal steroidogenesis reboot as a key event in the modulation of vizcacha pregnancy and depict yet another distinctive aspect of its reproductive endocrinology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Achieving full-term pregnancy in the vizcacha relies on a reboot of luteal steroidogenesis in mid-gestation (Lagostomus maximus, Rodentia)

et al. 2022

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“…In order to gather experimental groups during gestation, captures were planned according to the natural reproductive cycle previously described in Llanos and Crespo (1952) and in our own previous works (Charif et al, 2017;Dorfman et al, 2013;Fraunhoffer et al, 2017;Inserra et al, 2017;Leopardo et al, 2011;Proietto et al, 2019). Charif et al, 2017;Dorfman et al, 2013;Fraunhoffer et al, 2017;Leopardo et al, 2011;Proietto et al, 2019).…”

Section: Physiologic Approachmentioning

confidence: 99%

“…This hormonal fall enables the reactivation of the reproductive axis, probably beginning by the pulsatile release of GnRH, followed by the restoration of follicle-stimulating hormone and 17β-estradiol (E2) secretion (Dorfman et al, 2013;Fraunhoffer et al, 2017;Inserra et al, 2017). This event marks the beginning of the second stage of its pregnancy with a peak of LH around day 100, resulting in the recovery of the 3ß-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase enzyme steroidogenic activity in the corpora lutea and the increase of serum P4 levels (Cortasa et al, 2022;Fraunhoffer et al, 2017;Proietto et al, 2018Proietto et al, , 2019. This hormone secretion remains until the end of pregnancy favoring the full-term development of the only two fetuses that have bypassed the reabsorption process (Fraunhoffer et al, 2017;Jensen et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Hypothalamic GnRH expression and pulsatility depends on a balance of prolactin receptors in the plains vizcacha, Lagostomus maximus

Cortasa

Schmidt

Proietto

et al. 2023

J of Comparative Neurology

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In mammals, gestation is considered a physiological hyperprolactinemia status. Prolactin (PRL) is one of the modulators of gonadotropin‐releasing hormone (GnRH) neurons function. The South American plains vizcacha (Lagostomus maximus) is a unique model to study the regulation of hypothalamic GnRH neurons by direct and indirect steroid‐dependent pathways. The aim was to characterize the hypothalamic expression of endocrine markers in vizcacha during gestation as well as their response to experimental induced hyperprolactinemia. The possible involvement of PRL regulatory pathways on GnRH in the context of hypothalamic and pituitary reactivation in mid‐gestating vizcachas was discussed. Using two in vivo approaches, we determined changes in the hypothalamic expression and distribution of prolactin receptor (PRLR), tyrosine hydroxylase (TH), and dopamine type 2 receptor. A significant increment in the number of tuberoinfundibular dopaminergic (TIDA) neurons was determined in the arcuate nucleus from early to term pregnancy. On the other hand, at preoptic area, the number of both TH+PRLR+ and GnRH+PRLR+ double‐labeled neurons significantly decreased at mid‐pregnancy probably allowing the recovery of GnRH expression indicating that both types of neurons may represent the key points of PRL indirect and direct pathways modulating GnRH. Moreover, in a model of induced hyperprolactinemic vizcachas, the inhibitory effect of PRL on GnRH at both expression and delivery levels were confirmed. These results suggest the concomitant participation of both PRL regulatory pathways on GnRH modulation and pinpoint the key role of PRL on GnRH expression enabling the recovery of the hypothalamic activity during the gestation in this species.

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“…Subsequently, he served as a Guest Researcher at the Lerner Research Institute of the Cleveland Clinic Foundation, Cleveland, USA, in the molecular biology of T-box genes in breast cancer and its role in mouse embryological development. Nowadays he works at the Center for Biomedical, Biotechnological, Environmental and Diagnostic Studies (CEBBAD) of the Maimonides University, conducts research on the specification of germ cells in eutherian mammals (Proietto et al, 2019).…”

Section: History Of Developmental Biology In Argentina 13mentioning

confidence: 99%

Early and late stages of Developmental Biology in Argentina

Sánchez¹,

Honoré²

2021

Int. J. Dev. Biol.

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The history of science in Argentina is based on the enormous contribution that the great immigration of the 19th and 20th centuries produced in the country. The scientific and philosophical ideas and the role played especially by Italian scientists who arrived in the country produced a great impact on the different disciplines including development biology in emerging universities. The University of Tucumán pioneered the study of experimental biology, making important contributions to reproductive biology and to the early development of amphibians. The contribution of the Italian embryologist Prof. Armando Pisanó and the Argentinian Francisco D. Barbieri expanded the field to other universities and research centers located in Córdoba, La Plata, Bahía Blanca and Rosario. Given its strategic position, laboratories located in Buenos Aires city reached technological advances faster than others. Indeed, these laboratories saw the evolution from experimental biology to developmental genetics, renewing interest in this area. Currently, Developmental Biology brings together young researchers eager to consolidate regional and global collaboration networks that seek to help solve specific problems such as fertility, epigenetics, stem cells and tissue engineering.

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Pituitary estrogen receptor alpha is involved in luteinizing hormone pulsatility at mid-gestation in the South American plains vizcacha, Lagostomus maximus (Rodentia, Caviomorpha)

Cited by 11 publications

References 54 publications

Achieving full-term pregnancy in the vizcacha relies on a reboot of luteal steroidogenesis in mid-gestation (Lagostomus maximus, Rodentia)

Achieving full-term pregnancy in the vizcacha relies on a reboot of luteal steroidogenesis in mid-gestation (Lagostomus maximus, Rodentia)

Hypothalamic GnRH expression and pulsatility depends on a balance of prolactin receptors in the plains vizcacha, Lagostomus maximus

Early and late stages of Developmental Biology in Argentina

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