Pituitary adenylate cyclase-activating polypeptide (PACAP)-like immunoreactivity in the brain of a teleost, Uranoscopus japonicus: immunohistochemical relationship between PACAP and adenohypophysial hormones

Matsuda, Kouhei; Nagano, Yoshinobu; Uchiyama, Minoru; Onoue, Satomi; Takahashi, Akiyoshi; Kawauchi, Hiroshi; Shioda, Seiji

doi:10.1016/j.regpep.2004.08.019

Cited by 24 publications

(5 citation statements)

References 51 publications

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“…For immunostaining of the SL in the PVDF membrane, we used anti-SL serum (diluted 1:8000). Previous pre-absorption tests of anti-SL serum resulted in no specific staining; the specificity of this antiserum has been well demonstrated in teleost pituitaries and checked by immunoblotting analysis and RIA , Matsuda et al 2005a,b, 2008. In brief, each membrane was treated with 10% BSA (Sigma-Aldrich) in 0 .…”

Section: Primary Cell Culture and Cell Immunoblot Methodsmentioning

confidence: 93%

Melanin-concentrating hormone reduces somatolactin release from cultured goldfish pituitary cells

Tanaka¹,

Azuma²,

Nejigaki³

et al. 2009

Journal of Endocrinology

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Melanin-concentrating hormone (MCH)-containing neurons directly innervate the adenohypophysis in the teleost pituitary. We examined immunohistochemically the relationship between MCH-containing nerve fibres or endings and somatolactin (SL)-producing cells in the goldfish pituitary. Nerve fibres or endings with MCH-like immunoreactivity were identified in the neurohypophysis in close proximity to the adenohypophysial cells showing SL-like immunoreactivity. We also examined the effect of MCH on SL release from cultured goldfish pituitary cells and SL synthesis using a cell immunoblot and a real-time PCR method. Treatment of individually dispersed pituitary cells with MCH 10 K7 M for 3 h decreased the area of SL-like immunoreactivity on immunoblots, and MCH-induced reductions in SL release were blocked by treatment with the mammalian MCH receptor (MCHR) antagonist, compound-30, at a concentration of 10 K5 M.Treatment with 10 K7 M MCH for 3 h did not affect sl-a and -b (smtla and -b as given in the Zfin Database) mRNA expression levels. These led us to explore the signal transduction mechanism leading to the inhibition of SL release, for which we examined whether MCH-induced reductions in SL release are mediated by the G i or G q proteincoupled signalling pathway. The MCH-induced reductions in SL release were abolished by treatment with the G i/o protein inhibitors, NF023 (10 K5 M) or pertussis toxin (260 ng/ml), but not by the phospholipase C inhibitor, U-73122 (3!10 K6 M). These results indicate that MCH can potentially function as a hypothalamic factor suppressing SL release via the MCHR, and subsequently through the G i protein to inhibit the adenylate cyclase/cAMP/protein kinase A-signalling pathway in goldfish pituitary cells.

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Section: Primary Cell Culture and Cell Immunoblot Methodsmentioning

confidence: 93%

Melanin-concentrating hormone reduces somatolactin release from cultured goldfish pituitary cells

Tanaka¹,

Azuma²,

Nejigaki³

et al. 2009

Journal of Endocrinology

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“…S. aurata GH mRNA levels varied between the increase, the decrease, or even the complete loss of responsiveness (Laiz-Carrión et al 2009;VargasChacoff et al 2009b). PACAP, however, stimulates the in vitro and in vivo GH release from the pituitary gland of some fish species, such as the goldfish Carassius auratus, the stargazer Uranoscopus japonicus, the common carp Cyprinus carpio, the turbot Scophthalmus maximus and the sockeye salmon Oncorhynchus nerka (Parker et al 1997;Wong et al 1998;Rousseau et al 2001;Xiao et al 2002;Matsuda et al 2005). However, GH mRNA did not change under PACAP treatment in the olive flounder Paralichthys olivaceus (Nam et al 2013).…”

Section: Gh/igf-i Axismentioning

confidence: 95%

Acclimation to different environmental salinities induces molecular endocrine changes in the GH/IGF-I axis of juvenile gilthead sea bream (Sparus aurata L.)

2014

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To assess the role of the GH/IGF-I axis in osmotic acclimation of the gilthead seabream Sparus aurata, juvenile specimens were acclimated to four environmental salinities: hyposmotic (5 ‰), isosmotic (12 ‰) and hyperosmotic (40 and 55 ‰). The full-length cDNAs for both pituitary adenylate cyclase-activating peptide (PACAP) and prepro-somatostatin-I (PSS-I), the precursor for mature somatostatin-I (SS-I), were cloned. Hypothalamic PACAP and PSS-I, hypophyseal growth hormone (GH) and prolactin (PRL), and hepatic insulin-like growth factor-I (IGF-I) mRNA expression levels were analyzed in the four rearing salinities tested. PACAP and IGF-I mRNA values increased significantly in response to both 5 and 55 ‰ salinities, showing a U-shaped curve relationship with the basal level in the 40 ‰ group. Hypothalamic PSS-I expression increased strongly in the 55 ‰ environment. GH mRNA levels did not change in any of the tested environmental salinities. PRL mRNA maximum levels were encountered in the 5 and 12 ‰ environments, but significantly down-regulated in the 40 ‰. Plasma cortisol levels significantly increased in the 40 ‰ environment. These results are discussed in relation to the well-known high adaptability of Sparus aurata to different environmental salinities and the role of the GH/IGF-I axis in this process.

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“…This peptide is found from invertebrates to humans. PACAP was demonstrated in various non-mammalian vertebrates such as different species of fish (8)(9)(10)(11), frogs (12)(13)(14)(15)(16)(17) and birds (18,19). PACAP was able to stimulate AC in frog anterior pituitary cells (13,14,20).…”

Section: Introductionmentioning

confidence: 99%

Current State of Understanding of the Role of PACAP in the Hypothalamo-Hypophyseal Gonadotropin Functions of Mammals

et al. 2020

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PACAP was discovered 30 years ago in Dr. Akira Arimura's laboratory. In the past three decades since then, it has become evident that this peptide plays numerous crucial roles in mammalian organisms. The most important functions of PACAP are the following: 1. neurotransmitter, 2. neuromodulator, 3. hypophysiotropic hormone, 4. neuroprotector. This paper reviews the accumulated data regarding the distribution of PACAP and its receptors in the mammalian hypothalamus and pituitary gland, the role of PACAP in the gonadotropin hormone secretion of females and males. The review also summarizes the interaction between PACAP, GnRH, and sex steroids as well as hypothalamic peptides including kisspeptin. The possible role of PACAP in reproductive functions through the biological clock is also discussed. Finally, the significance of PACAP in the hypothalamo-hypophysial system is considered and the facts missing, that would help better understand the function of PACAP in this system, are also highlighted.

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Pituitary adenylate cyclase-activating polypeptide (PACAP)-like immunoreactivity in the brain of a teleost, Uranoscopus japonicus: immunohistochemical relationship between PACAP and adenohypophysial hormones

Cited by 24 publications

References 51 publications

Melanin-concentrating hormone reduces somatolactin release from cultured goldfish pituitary cells

Melanin-concentrating hormone reduces somatolactin release from cultured goldfish pituitary cells

Acclimation to different environmental salinities induces molecular endocrine changes in the GH/IGF-I axis of juvenile gilthead sea bream (Sparus aurata L.)

Current State of Understanding of the Role of PACAP in the Hypothalamo-Hypophyseal Gonadotropin Functions of Mammals

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