Pituitary adenylate cyclase-activating peptide (PACAP) immunoreactivity distribution in the small intestine of the adult New Hampshire chicken

Pirone, Andrea; Ding, Baoan; Piano, Ilaria; Santina, Luca Della; Baglini, Alessandro; Lenzi, Carla

doi:10.1016/j.acthis.2010.04.008

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…It is said that the inhibition of intestinal motility by GLP‐1 comes out through an indirect effect via central and enteric nervous system (Tolessa et al., ,b; Stanley et al., ). Dense network of pituitary adenylate cyclase‐activating peptide (PACAP)‐immunoreactive nerve fibres was found in the lamina propria of villi of chicken small intestine (Pirone et al., ). These findings indicate the close relationship between enteroendocrine cells in epithelium and nervous elements in the lamina propria .…”

Section: Discussionmentioning

confidence: 99%

The Influence of Restricted Feeding on Glucagon‐Like Peptide‐1 (GLP‐1)‐Containing Cells in the Chicken Small Intestine

Monir

Hiramatsu

Yamasaki

et al. 2013

Anat Histol Embryol

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The influence of restricted feeding on the distribution of glucagon-like peptide-1 (GLP-1)-containing endocrine cells in the chicken small intestine was investigated using immunohistochemical and morphometrical techniques. This study demonstrated that the restricted feeding had an influence on the activity of GLP-1-immunoreactive cells in the chicken small intestine. There were differences in the localization and the frequency of occurrence of GLP-1-immunoreactive cells in the small intestine between control and restricted groups, especially 25% feed supply group provided with 25% of the intake during the adapting period. GLP-1-immunoreactive cells in the control chickens were mainly located in epithelium from crypts to the lower part of intestinal villi. Those in restricted groups, however, tended to be located from crypts to the middle part of intestinal villi. The frequency of occurrence of GLP-1-immunoreactive cells was lowest in the control group, medium in 50% feed supply group and highest in 25% feed supply group at each intestinal region examined in this study, that is, increased with the advancement of restricting the amount of feed supply. These data show that the quantity of food intake is one of signals that have an influence on the secretion of GLP-1 from L cells in the chicken small intestine.

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Section: Discussionmentioning

confidence: 99%

The Influence of Restricted Feeding on Glucagon‐Like Peptide‐1 (GLP‐1)‐Containing Cells in the Chicken Small Intestine

Monir

Hiramatsu

Yamasaki

et al. 2013

Anat Histol Embryol

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“…The active neuropeptide comprises 38 amino acid residues (PACAP 1-38); however, a shorter α-amidated form that corresponds to the first 27 residues at the N-terminus (PACAP 1-27) also exists, suggesting that their biologically active region is completely preserved during evolution [1]. PACAP 1-38, the dominant form of the neuropeptide, is widely distributed in the central nervous system (CNS) and is also present in several peripheral tissues such as gonads [2], intestinal system [3], urinary tract [4], as well as in fluid compartments including blood plasma [5] and human milk [6]. In spite of the large variety of tissues containing or releasing PACAP, there are only sporadic data about its function in skeletal elements such as bone [7]–[9]; in particular, its presence in hyaline cartilage has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Signalling Exerts Chondrogenesis Promoting and Protecting Effects: Implication of Calcineurin as a Downstream Target

et al. 2014

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Pituitary adenylate cyclase activating polypeptide (PACAP) is an important neurotrophic factor influencing differentiation of neuronal elements and exerting protecting role during traumatic injuries or inflammatory processes of the central nervous system. Although increasing evidence is available on its presence and protecting function in various peripheral tissues, little is known about the role of PACAP in formation of skeletal components. To this end, we aimed to map elements of PACAP signalling in developing cartilage under physiological conditions and during oxidative stress. mRNAs of PACAP and its receptors (PAC1,VPAC1, VPAC2) were detectable during differentiation of chicken limb bud-derived chondrogenic cells in micromass cell cultures. Expression of PAC1 protein showed a peak on days of final commitment of chondrogenic cells. Administration of either the PAC1 receptor agonist PACAP 1-38, or PACAP 6-38 that is generally used as a PAC1 antagonist, augmented cartilage formation, stimulated cell proliferation and enhanced PAC1 and Sox9 protein expression. Both variants of PACAP elevated the protein expression and activity of the Ca-calmodulin dependent Ser/Thr protein phosphatase calcineurin. Application of PACAPs failed to rescue cartilage formation when the activity of calcineurin was pharmacologically inhibited with cyclosporine A. Moreover, exogenous PACAPs prevented diminishing of cartilage formation and decrease of calcineurin activity during oxidative stress. As an unexpected phenomenon, PACAP 6-38 elicited similar effects to those of PACAP 1-38, although to a different extent. On the basis of the above results, we propose calcineurin as a downstream target of PACAP signalling in differentiating chondrocytes either in normal or pathophysiological conditions. Our observations imply the therapeutical perspective that PACAP can be applied as a natural agent that may have protecting effect during joint inflammation and/or may promote cartilage regeneration during degenerative diseases of articular cartilage.

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“…[8][9] Mindhárom receptoruk megtalálható a mucosában, a myentericus neuronokban, neuroendokrin és érendothelsejteken, valamint a simaizomsejteken. [10][11] A PACAP-38 antiapoptotikus, antiinflammatórikus és antioxidáns protektív hatása számos in vitro és in vivo kí-sérletben igazolódott. [12][13][14][15] Pontosan még nincs tisztázva, hogy milyen mechanizmusokon keresztül hat, de az igen, hogy a bélszöveti I/R-s károsodás a béltraktusban citokinfelszabadulást eredményez.…”

Section: Bevezetésunclassified

A PACAP-38 citoprotektív és antiinflammatórikus hatásának vizsgálata vékonybél-autotranszplantációs modellben

Nedvig

Szabó

Csukás

et al. 2013

MaSeb

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Bevezetés: A vékonybél ischaemia-reperfusióval szembeni fokozott érzékenysége a szerv transzplantációjakor is jelen lévő probléma. Ismert a hypophysis adenilát-cikláz aktiváló polipeptid (PACAP) sejtvédő hatása. Munkánkban azt vizsgáltuk, hogy PACAP-38-at tartalmazó University of Wisconsin (UW) oldatban történő konzerválás hogyan befolyá-solja a szöveti PACAP-és citokinszinteket. Anyag és módszer: Wistar-patkányokon (n = 56) vékonybél-autotranszplantációt végeztünk. A graftokat 4 °C-os UW oldatban tároltuk 1 (I. csoport), 3 (II.) és 6 órán (III.), illetve 100 μg PACAP-38-at tartalmazó UW oldatban 1 (IV.), 3 (V.) és 6 órán (VI.) át. A reperfusio 3 óra volt. Bélmintákból a PACAP-38-és PACAP-27-szinteket radioimmunassayjel határoztuk meg, míg a citokinexpressiót kemilumineszcens módszerrel és Luminex Multiplex Immunoassayjel mértük. Eredmények: A szöveti PACAP-38-szint a kontrollhoz (57,32 ± 3,5 fmol/mg) képest a konzerválás idejével csökkent, és 6 óra után szignifikáns volt (III.: 32,6 ± 3,9 fmol/mg, p < 0,05), míg a IV-VI. csoportoknál szignifikánsan nőtt. A PACAP-27 szöveti értéke is hasonló tendenciával változott. Az sICAM-1, L-selectin és a metalloproteáz-1 szöveti inhibitorának emelkedett expressióját mértük a III. csoportban, és jelentős csökkenés volt a VI. csoportban. Következtetés: UW oldathoz adott PACAP-38 növelte a szöveti PACAP-38-és PACAP-27-szinteket, és csökkentette a citokinexpressiót. Mindez a PACAP-38 citoprotektív és anti-inflammatórikus hatását jelzi bél-autotranszplantációs modellben.Támogatta: OTKA (PD77474, 104984, CNK78480), MTA Bolyai-ösztöndíj és Lendület program. Kulcsszavak: vékonybél, transzplantáció, PACAP-38, PACAP-27, citokinexpressioIntroduction: The small intestine is one of the most sensitive organs to ischemia-reperfusion injury during transplantation. Cytoprotective effect of pituitary adenylate cyclase-activating polypeptide (PACAP) is well known. The aim of our study was to measure changes of PACAP-38-like immunoreactivities and cytokine levels in intestinal grafts stored PACAP-38 containing preservation solution. Material and methods: Small-bowel autotransplantation was performed on male Wistar rats (n = 56). Grafts were stored in University of Wisconsin (UW) solution at 4 °C for 1 (GI), 3 (GII), and 6 hours (GIII); and in PACAP-38 containing UW solution for 1 (GIV), 3 (GV), and 6 hours (GVI). Reperfusion lasted 3 hours in each group. Intestinal PACAP-38 immunoreactivities were measured by radioimmunoassay. To measure cytokine from tissue homogenates we used rat cytokine array and Luminex Multiplex Immunoassay. Results: Levels of PACAP-38-like and PACAP-27-like immunoreactivities decreased by preservation time compared to control. This decrease was significant following 6 hours cold storage (p < 0.05). Values remained significantly higher in grafts stored in PACAP-38 containing UW. Expressions of sICAM-1, L-selectin, tissue inhibitor of metalloproteinase-1 were increased in GIII and were decreased in GVI. Conclusion: PACAP-38 increased tissue levels of PACAP-38 and PACAP-27...

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Pituitary adenylate cyclase-activating peptide (PACAP) immunoreactivity distribution in the small intestine of the adult New Hampshire chicken

Cited by 13 publications

References 37 publications

The Influence of Restricted Feeding on Glucagon‐Like Peptide‐1 (GLP‐1)‐Containing Cells in the Chicken Small Intestine

The Influence of Restricted Feeding on Glucagon‐Like Peptide‐1 (GLP‐1)‐Containing Cells in the Chicken Small Intestine

Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Signalling Exerts Chondrogenesis Promoting and Protecting Effects: Implication of Calcineurin as a Downstream Target

A PACAP-38 citoprotektív és antiinflammatórikus hatásának vizsgálata vékonybél-autotranszplantációs modellben

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