"Pittsburgh Pneumonia Agent": A Bacterium Phenotypically Similar to <i>Legionella pneumophila</i> and Identical to the TATLOCK Bacterium

Hébert, Guillaume; Thomason, Berenice M.; Harris, P P; Hicklin, Martin D.; McKinney, Roger M.

doi:10.7326/0003-4819-92-1-53

Cited by 62 publications

(22 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From tissues, Tatlockia may be partly acid-fast (20). Although motility of some strains has been reported (10) and polar flagella have been demonstrated (10,12,20), these organisms are usually nonmotile after agar passage. Motility of some strains may be restored by passage on semisolid media.…”

Section: Resultsmentioning

confidence: 99%

Tatlockia and Fluoribacter: Two New Genera of Organisms Resembling Legionella pneumophila

Brown

Vickers

1980

International Journal of Systematic Bacteriology

106

View full text Add to dashboard Cite

DNA homology studies were performed with strains of Legionella pneumophila Brenner et al., 1979, including the type strain, ATCC 33152, and a group of seven previously unclassified bacteria which resemble L. pneumophila in growth characteristics and colonial morphology and by their content of large amounts of branched-chain fatty acids. The organisms studied include three isolates of the Pittsburgh pneumonia agent (PPA), TATLOCK, WIGA (ALLOI), MI-15 (ALLOZ), and NY-23 (ALLOJ. The purified DNAs were labeled with 32P in vitro by a nick translation method, which made it possible to achieve very high specific activities. Results of our hybridization experiments suggest that these previously unclassified organisms are not related to L. pneumophila at the genus level, and thus two new genera within the family Legionellaceae are proposed for them: Tatlockia and Fluoribacter. The type species of these genera are T. micdadei (Hebert et al., 1980) comb. nov. and F. bozemanae sp. nov., respectively. The type strain of 2 ' . micdadei is strain TATLOCK (= ATCC 33218), and the type strain of F. bozemanae is strain WIGA (= ATCC 33217).Legionnaires' disease, first recognized during an outbreak in Philadelphia in 1976 (9), has been shown to be due to infection with a bacterium (15) for which the name Legionella pneumophila has been proposed (5). This organism is gram negative and requires cysteine, iron salts, and a medium buffered at pH 6.9 for optimal growth (8).Since the isolation of L. pneumophila, intensive study of previously unclassified organisms (1, 10, 16, 24) and a search for new isolates (7, 12, 14, 21, 25) from both human and environmental sources has led to the recognition of a number of organisms resembling L. pneumophila with respect to nutritional requirements and colonial morphology (7, 12, 14). Of these organisms, L. pneurnophila, WIGA (ALLOI), Pittsburgh pneumonia agent (PPA), HEBA, and TATLOCK possess common flagellar antigens (10, 12), a relationship which may exist among all flagellated members of this group of organisms and which may be of taxonomic importance. Cellular fatty acid analysis disclosed the presence of branched-chain fatty acids in unusually large amounts for gram-negative organisms (19), and characteristic fatty-acid patterns were described for L. pneumophila and for several others of these bacteria (7, 10, 14, 18, 19).In this article, we present extensive deoxyribonucleic acid (DNA)-DNA homology data in an attempt to determine the relatedness among members of this group. Since the original submission of this manuscript for publication, two articles have appeared proposing scientific names for the organisms referred to as TAT-LOCK and PPA (11,20); one of the articles also refers to the name L. bozemanii for the WIGA bacterium, but a formal proposal is not made (11). Both sets of authors have placed these organisms in the genus Legionella. Our data, as well as the available data presented in these other reports, do not support the classification of these organisms into a single genus, but arg...

show abstract

Section: Resultsmentioning

confidence: 99%

Tatlockia and Fluoribacter: Two New Genera of Organisms Resembling Legionella pneumophila

Brown

Vickers

1980

International Journal of Systematic Bacteriology

106

View full text Add to dashboard Cite

show abstract

“…Since the discovery of Legionella pneumophila, the agent of Legionnaires disease, two other groups of fastidious bacteria have been isolated (1,7,9,13,19,21,22) which share some phenotypic characteristics with L. pneumophila (6,7,11,13). However, deoxyribonucleic acid (DNA) hybridization studies demonstrated no sigrzlficant homology between these strains and L. pneumophila (5, 8-10, 13-15, 18), thereby excluding relatedness at the genus level.…”

mentioning

confidence: 99%

“…However, deoxyribonucleic acid (DNA) hybridization studies demonstrated no sigrzlficant homology between these strains and L. pneumophila (5, 8-10, 13-15, 18), thereby excluding relatedness at the genus level. One group contains the strains known as the Pittsburgh pneumonia agent (PPA) (11,19) and the TATLOCK strain (9, 11, Z l ) , for which three scientific names, Legionella micdadei, L. pittsburghensis (18), and Tatlockia micdadei (8), have been proposed. Members of the second group are serologically more diverse (7,13) and have been referred to as atypical Legionella-like organisms (ALLO) (7).…”

mentioning

confidence: 99%

Fluoribacter dumoffii (Brenner et al.) comb. nov. and Fluoribacter gormanii (Morris et al.) comb. nov.

Brown

Vickers

1981

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

“…This organism has now been cultivated on artificial media and shown to be phenotypically similar to, but genetically and antigenically distinct from, Legionella pneumophila (13,18). The names Legionella micdadei (12) and Legionellapittsburgensis (18) have been proposed for this organisim and two similar isolates, TAT-LOCK and HEBA (11).…”

mentioning

confidence: 99%

“…Analysis of the antibiotic therapy given patients in the clusters of L. micdadei pneumonia which occurred in Pittsburgh, Pa., and Charlottesville, Va., did not reveal any pattern of antimicrobial efficacy (17,20). However, many of these patients received beta-lactam and aminoglycoside antibiotics, both alone and in combination, to which L. micdadei displays marked in vitro susceptibility, and yet 8 of these 13 patients died. Susceptibility testing of L. micdadei with an egg protection model has shown that beta-lactam and aminoglycoside antibiotics are also not effective in ovo (17).…”

mentioning

confidence: 99%

Susceptibility of Pittsburgh pneumonia agent (Legionella micdadei) and other newly recognized members of the genus Legionella to nineteen antimicrobial agents

Pasculle¹,

Dowling²,

Weyant³

et al. 1981

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The susceptibilities of 11 strains representing the five recognized species of Legionella were determined by agar dilution testing on buffered charcoal-yeast extract agar. All of the legionellae tested were susceptible to rifampin, erythromycin, rosaramycin, chloramphenicol, and the aminoglycosides and were resistant to clindamycin and vancomycin. Susceptibilities to penicillins and cephalosporins were variable. Legionella micdadei, Legionella bozemanii, and Legionella gormanii were susceptible to these agents, but minimal inhibitory concentrations for each species were different. Legionella dumoffii resembled Legionella pneumophila in being resistant to penicillin, cephalothin, and cephamandole and susceptible to moxalactam and cefoxitin. All species except L. micdadei produced beta-lactamase.

show abstract

"Pittsburgh Pneumonia Agent": A Bacterium Phenotypically Similar to Legionella pneumophila and Identical to the TATLOCK Bacterium

Cited by 62 publications

References 12 publications

Tatlockia and Fluoribacter: Two New Genera of Organisms Resembling Legionella pneumophila

Tatlockia and Fluoribacter: Two New Genera of Organisms Resembling Legionella pneumophila

Fluoribacter dumoffii (Brenner et al.) comb. nov. and Fluoribacter gormanii (Morris et al.) comb. nov.

Susceptibility of Pittsburgh pneumonia agent (Legionella micdadei) and other newly recognized members of the genus Legionella to nineteen antimicrobial agents

Contact Info

Product

Resources

About