Pitfalls in the AR42J-model of cerulein-induced acute pancreatitis

Hollenbach, Marcus; Sonnenberg, Sebastian; Sommerer, Ines; Lorenz, Jana; Mössner, Joachim; Hoffmeister, Albrecht

doi:10.1016/j.pan.2019.05.273

Cited by 2 publications

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“…AR42J are immortalized pancreatic adenocarcinoma cells that share similarities with pancreatic acinar cells. AR42J is often used as a cell-culture model of cerulein-(CN-) induced acute pancreatitis (AP) [13]. Ibuprofen is a widely used and well-tolerated analgesic and antipyretic.…”

Section: Discussionmentioning

confidence: 99%

Ibuprofen Alleviates Acute Pancreatitis– (AP–) Induced Myocardial Injury by Inhibiting AIM2

Chen

Wang

2022

Computational and Mathematical Methods in Medicine

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Objective.The lack of certain trace elements such as selenium, molybdenum, magnesium or related nutrients in the soil, water quality and food in the disease area, which caused disturbance of myocardium metabolism and resulted in injury and necrosis. The aim of the study was to explore the mechanism of ibuprofen alleviating myocardial injury caused by acute pancreatitis (AP). Method. We have established AP cell model and rat model. HE staining is used for histological examination. ELISA is used to determine the levels of proinflammatory cytokines (TNF-α and IL-6) and markers of myocardial injury (LDH and CK-MB). qRT-PCR and Western blot are used to analyze the mRNA and protein levels of related genes. Results. The expression level of AIM2 was significantly increased in AP cells; downregulation of AIM2 alleviated inflammation and myocardial injury induced by AP cells; ibuprofen could inhibit the expression of AIM2 and alleviate inflammation and myocardial injury induced by AP cells. In vivo experiments have found that ibuprofen can inhibit the expression of AIM2 to alleviate myocardial injury in AP rat. Conclusion. Ibuprofen can alleviate myocardial injury caused by acute pancreatitis by inhibiting the expression of AIM2.

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Section: Discussionmentioning

confidence: 99%

Ibuprofen Alleviates Acute Pancreatitis– (AP–) Induced Myocardial Injury by Inhibiting AIM2

Chen

Wang

2022

Computational and Mathematical Methods in Medicine

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“…Among many rat cell lines, the AR42J cell has been most widely used, presumably because of its dual properties in both exocrine and endocrine lineages 20 . It allows functional analysis as a substitute for normal islet cells and acinar cells, 21 which are still difficult to maintain in culture. Genetically engineered mice represent an alternative in vivo models, having pancreas‐specific overexpression of SV40 22 and ablation of Tsc1 , either alone 23 or in combination with Trp53 deletion 24 .…”

Section: Introductionmentioning

confidence: 99%

Derivation of pancreatic acinar cell carcinoma cell line HS‐1 as a patient‐derived tumor organoid

et al. 2022

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Acinar cell carcinoma (ACC) of the pancreas is a malignant tumor of the exocrine cell lineage with a poor prognosis. Due to its rare incidence and technical difficulties, few authentic human cell lines are currently available, hampering detailed investigations of ACC. Therefore, we applied the organoid culture technique to various types of specimens, such as bile, biopsy, and resected tumor, obtained from a single ACC patient.Despite the initial propagation, none of these organoids achieved long-term proliferation or tolerated cryopreservation, confirming the challenging nature of establishing ACC cell lines. Nevertheless, the biopsy-derived early passage organoid developed subcutaneous tumors in immunodeficient mice. The xenograft tumor histologically resembled the original tumor and gave rise to infinitely propagating organoids with solid features and high levels of trypsin secretion. Moreover, the organoid stained positive for carboxylic ester hydrolase, a specific ACC marker, but negative for the duct cell marker CD133 and the endocrine lineage marker synaptophysin. Hence, we concluded the derivation of a novel ACC cell line of the pure exocrine lineage,

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Pitfalls in the AR42J-model of cerulein-induced acute pancreatitis

Cited by 2 publications

References 0 publications

Ibuprofen Alleviates Acute Pancreatitis– (AP–) Induced Myocardial Injury by Inhibiting AIM2

Ibuprofen Alleviates Acute Pancreatitis– (AP–) Induced Myocardial Injury by Inhibiting AIM2

Derivation of pancreatic acinar cell carcinoma cell line HS‐1 as a patient‐derived tumor organoid

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