Pitfalls in the 3, 5-dinitrosalicylic acid (DNS) assay for the reducing sugars: Interference of furfural and 5-hydroxymethylfurfural

Effects of different drying methods on the physicochemical property and edible quality of fermented Pyracantha fortuneana fruit powder

“…Soluble total sugar and reducing sugar, total acid (malic acid) and total free amino acids were, respectively, measured by 3,5‐dinitrosalicylic acid method (Deshavath et al ., 2020), NaOH titration method (Guo et al ., 2019) and ninhydrin colorimetric method (Wang et al ., 2018).…”

Characterization of a microbial consortium with potential for biological degradation of cactus pear biomass for biofuel production

“…In order to reinforce our working hypothesis that the microbial consortia and isolated strains excreted enzymes involved in cladode digestion, we assayed directly the culture supernatants from eubacterial and fungal isolates that showed the highest digestion activities for both cellulase and pectinase using a modified 3,5-dinitrosalicylic acid (DNS) reagent ( Miller, 1959 ) and 0.5% w/v carboxymethylcellulose or 0.5% w/v citrus pectin as substrates ( Deshavath et al., 2020 ; Vatanparast et al., 2012 ). Whereas only trace amounts of cellulase activity were detected in the culture supernatants of either the five-day-old, eubacterial or fungal consortia or individual axenic strains ( Figure 7 A, B), detectable pectinase activities were clearly present ( Figure 7 C, D).…”

“…A modified, phenol-free, colorimetric assay was used for the detection of cellulase and pectinase activities using 3, 5-dinitrosalicylic acid (DNS) as the reagent ( Miller, 1959 ) with 0.5% carboxymethyl cellulose (CMS, Sigma-Aldrich) and 0.5% soluble citrus pectin (Sigma-Aldrich) as substrates ( Deshavath et al., 2020 ; Vatanparast et al., 2012 ). DNS reagent was prepared by adding 1.6 g NaOH to 75 ml of deionized water until dissolved with stirring, followed by 1 g (1% w/v) DNS (Sigma-Aldrich) with continuous stirring.…”

Effect of Hydroxymethylfurfural and Low-Molecular-Weight Chitosan on Formation of Acrylamide and Hydroxymethylfurfural during Maillard Reaction in Glucose and Asparagine Model Systems

“…of reducing sugars was also observed in the 0.5% hydroxymethylfurfural (HMF) solution and HMF-asparagine solution (Figure S1A). The presence of HMF and furfural in solution indicated by magnified absorption values in 3,5-dinitrosalicylic acid (DNS) assay for reducing sugars [22]. However, the estimation of reducing sugars by DNS assay did differ significantly after 30 min of heating (Figure S1A).…”