piRNA hsa_piR_019914 promoted chondrocyte anabolic metabolism by inhibiting LDHA-dependent ROS production

Abstract

Objectives As a new molecular marker and therapeutic target, Piwi-interacting RNA(piRNA) has been widely studied in the field of oncology, but few people have studied the role of piRNA in osteoarthritis. Here, we found that the expression of hsa_piR_019914 in human osteoarthritis cartilage decreased significantly. We studied the relationship between hsa_piR_019914 and gene expression in osteoarthritis cartilage and the role of hsa_piR_019914 in chondrocyte biology. It is suggested that hsa_piR_019914 may be a new target for the treatment of osteoarthritis. Design Using the GEO database to analyze the expression of piRNA in the cartilage of osteoarthritis, using qPCR to detect the effect of inflammatory factors on the expression of piRNA in chondrocytes, using CCK-8 and clone formation to detect the effect of hsa_piR_019914 on chondrocyte proliferation, and using flow cytometry to detect the effect of hsa_piR_019914 on chondrocyte apoptosis and ROS production. The target gene of hsa_piR_019914 regulation was detected by mRNA sequencing. Results GEO data analysis found that 11 piRNAs were downregulated in cartilage tissue of osteoarthritis, and inflammatory factors inhibited the expression of hsa_piR_019914 in chondrocytes. Hsa_piR_019914 promoted the proliferation of chondrocytes and the synthesis of extracellular matrix. Hsa_piR_019914 inhibited the apoptosis of chondrocytes and the expression of cell-matrix protease. Hsa_piR_019914 targets regulating the expression of LDHA and inhibiting the production of ROS. Conclusions This work highlights the role of hsa_piR_019914 in chondrocyte metabolism, the inhibition of LDHA, and the reduction of ROS production, which may be used as a potential treatment for osteoarthritis.