Pirikool® 300 CS, a new long-lasting capsule suspension formulation of the organophosphate insecticide pirimiphos-methyl for indoor residual spraying against pyrethroid-resistant malaria vectors

Abstract

Background Indoor residual spraying (IRS) using a capsule suspension formulation of the organophosphate insecticide, pirimiphos-methyl, has provided substantial malaria control in many communities in Africa. However, only one brand of this product has been recommended by the World Health Organisation for IRS. To help increase the diversity of the portfolio of IRS insecticides and offer suitable options to procurers and malaria vector control programmes, additional product brands of this highly effective and long-lasting insecticide formulation for IRS will be needed. Methods We evaluated the efficacy of Pirikool® 300CS, a new capsule suspension formulation of pirimiphos-methyl developed by Tianjin Yorkool, International Trading, Co., Ltd in standard WHO laboratory bioassays and experimental hut studies. The efficacy of the insecticide applied at 1000mg/m2 was assessed in laboratory bioassays for 6 months on cement, plywood and mud block substrates and for 12 months in cement and mud-walled experimental huts against wild free-flying pyrethroid-resistant Anopheles gambiae sensu lato in Covè, Benin. Actellic® 300CS, a WHO-recommended capsule suspension formulation of pirimiphos-methyl was also tested. WHO cylinder tests were performed to determine the frequency of insecticide resistance in the wild vector population during the hut trial. Results The vector population at the hut station was resistant to pyrethroids but susceptible to pirimiphos-methyl. Overall mortality rates of wild free-flying pyrethroid-resistant An. gambiae (s.l.) entering Pirikool®300CS treated experimental huts during the 12-month trial were 86.7% in cement-walled huts and 88% in mud-walled huts. Mortality of susceptible An. gambiae (Kisumu) and pyrethroid-resistant An. gambiae s.l. (Covè) mosquitoes in monthly wall cone bioassays on Pirikool® 300CS treated hut walls remained over 80% for 10–12 months. The laboratory bioassays corroborated the hut findings with Pirikool® 300CS on mud and wood block substrates but not on cement block substrates. Conclusion Indoor residual spraying with Pirikool® 300CS induced high and prolonged mortality of wild pyrethroid-resistant malaria vectors for 10–12 months. Addition of Pirikool®300CS to the current portfolio of IRS insecticides will provide an extra choice of microencapsulated pirimiphos-methyl for IRS.

Efficacy of Pirikool® 300 CS used for indoor residual spraying on three different substrates in semi-field experimental conditions

“…Despite the Tiassalé vectors' insecticide tolerance, Pirikool® 300 CS demonstrated an excellent lethal impact with all three types of substrates. The mortality rates that occurred were equivalent to those achieved with the same substance in a similar research in Benin [ 18 ]. The mortality rate generated on wild mosquitos entering in the huts was higher on the wooden hut substrates than with the other two substrate types.…”

“…However, according to IRS ideas, vectors would often feed on the host prior to coming into touch with the insecticide, resulting in a high feeding rate, as shown throughout various evaluations [ 11 , 18 , 32 , 33 ].…”

“…In this context, Tianjin Yorkool International Trading Co, Ltd. has developed a new micro-encapsulated formulation (CS) of Pirimiphos-methyl called Pirikool® 300 CS that has a longer lasting effectiveness. This compound showed good performance in indoor residual spraying in experimental huts in Benin against pyrethroid-resistant vectors [ 18 ]. However, in some localities, such as Tiassalé in Côte d'Ivoire, malaria vector resistance is extended to all classes of public health insecticides [ 19 – 21 ].…”