Pir2/Rnf144b is a potential endometrial cancer biomarker that promotes cell proliferation

Zhou, Qing; Eldakhakhny, Sahar; Conforti, Franco; Crosbie, Emma J.; Melino, Gerry; Sayan, Berna S.

doi:10.1038/s41419-018-0521-1

Cited by 14 publications

(20 citation statements)

References 56 publications

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“…High rate of proliferation and improved wound healing [204] Oncogene [205] PY region just before the SAM domain of p73, and particularly the Y487 aa residue of TAp73 [219] 2005 [219] FBXO45 TAp73α SAM domain [223] 2009 [223] PIR2/RNF144B ∆Np73α Oncogene [224] 2010 [225] PIRH 2 TAp73α * TAp73β Predisposed to tumorigenesis [64] Oncogene [66,67,71] Tumor Suppressor [64] 2011 [166,167] *…”

Section: Itch Tap73α ∆Np73αmentioning

confidence: 99%

Regulation of the p53 Family Proteins by the Ubiquitin Proteasomal Pathway

Bang

Kaur

Kurokawa

2019

IJMS

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The tumor suppressor p53 and its homologues, p63 and p73, play a pivotal role in the regulation of the DNA damage response, cellular homeostasis, development, aging, and metabolism. A number of mouse studies have shown that a genetic defect in the p53 family could lead to spontaneous tumor development, embryonic lethality, or severe tissue abnormality, indicating that the activity of the p53 family must be tightly regulated to maintain normal cellular functions. While the p53 family members are regulated at the level of gene expression as well as post-translational modification, they are also controlled at the level of protein stability through the ubiquitin proteasomal pathway. Over the last 20 years, many ubiquitin E3 ligases have been discovered that directly promote protein degradation of p53, p63, and p73 in vitro and in vivo. Here, we provide an overview of such E3 ligases and discuss their roles and functions.

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Section: Itch Tap73α ∆Np73αmentioning

confidence: 99%

Regulation of the p53 Family Proteins by the Ubiquitin Proteasomal Pathway

Bang

Kaur

Kurokawa

2019

IJMS

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“…RNF144B also known as PIR2, is an E3-ubiquitin ligase involved in the regulation of cell apoptosis and proliferation. It has been recognized as important biomarker in endometrial cancer [36,37]. We observed a reduced expression of RNF144B in LUAD tissue, TCGA dataset and in lung cancer cell lines ( gure 6).…”

Section: Discussionmentioning

confidence: 71%

Integrated computational analysis revealed hub genes in lung adenocarcinoma

Jamal¹,

Khan²,

Bangash³

et al. 2021

Preprint

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Background Lung cancer (LUCA) is the leading cause of cancer-related morbidities and mortalities globally. Despite the recent advancements in lung cancer research, understanding of the molecular mechanism underlying LUCA tumorigenesis and prognosis remains suboptimal. This study aims to identify the candidate biomarkers and therapeutic genes in lung cancer. Methods In this study, gene expression profiles of GSE30219, GSE33532, GSE32863 and GSE43458 were downloaded from GEO. The differentially expressed genes (DEGs) in LUAD tissue and normal lung tissue with a p-value < 0.05 and a |log fold change (FC)| >1.0 were identified by GEO2R. For functional enrichment analysis of these DEGs, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed with KOBAS and DAVID tools. Next, the candidate hub genes were filtered out with Cytoscape using CytoHubba plugin. These hub genes were validated by (the Cancer Genome Atlas) TCGA-based gene expression analysis, protein-protein network interaction (PPI) analysis, survival analysis. Moreover, the expression of these genes in cancer and normal tissue was assessed in the Human Protein Atlas (HPA) database. In addition, miRNA network of the hub genes was constructed. Finally, DGIdb database was used to check the drug-targeting potentials of the hub genes. Results a total of 332 overlapping differentially expressed genes (DEGs) including 73 upregulated and 259 downregulated, respectively were identified. GO analysis revealed that the DEGs were principally regulating various cancer-associated functions and pathways. The module analysis revealed 55 hub genes in 4 modules. The survival analysis through Kaplan-Meier (KM) plotter indicated that the altered expression of these genes resulted in the poor overall survival (OS) of LUCA patients. Moreover, these genes show a differential expression on both protein and mRNA level in cancer patient compared to the normal. In addition, in addition, 6 potential microRNAs (miRNAs) interacting with hub genes were identified. Finally, a list of 117 therapeutic small molecules was tabulated that could facilitate LUCA treatment. Conclusions the findings of this study may help in the development of novel and reliable biomarkers for diagnosis, prognosis and therapeutic intervention for LUAD.

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“…RNF130 is a protein that has E3 ubiquitin ligase activity and is implicated in the modulation of biological activities, including, apoptosis, gene transcription, intracellular signal transduction, and DNA repair. Additionally, the overexpression of RNF130 has been identified in numerous cancers while the opposite expression was reported in other cancers [ 24 ]. Carbonic anhydrase IX (CA9), which belongs to the carbonic anhydrase family and is associated with hypoxic cancer cells, performs an instrumental function in the balance between intracellular and extracellular pH through chemical reactions and further produces an acidic extracellular microenvironment [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

Systematic Construction and Validation of a Novel Ferroptosis-Related Gene Model for Predicting Prognosis in Cervical Cancer

Qin

Jiang

et al. 2022

Journal of Immunology Research

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Background and Purpose. Ferroptosis, a mechanism of cell death that is iron-dependent, participates in various pathologies of cancer (CC). Nevertheless, the specific function that ferroptosis plays in the onset and progression of cervical cancer (CC) is yet uncertain. This research sought to examine the value of ferroptosis-related genes (FRGs) in the progression and prognosis of CC. Methods. Datasets containing RNA sequencing and corresponding clinical data of cervical cancer patients were obtained from searching publicly accessible databases. The “NMF” R package was conducted to calculate the matrix of the screened prognosis gene expression. Ferroptosis-related differential genes in cervical cancer were detected using the “limma” R function and WGCNA. The least absolute shrinkage and selection operator (LASSO) algorithm and Cox regression analysis were conducted to develop a novel prognostic signature. The prediction model was verified by the nomogram integrating clinical characteristics; the GSE44001 dataset was used as an external verification. Then, the immune status and tumor mutation load were explored. Finally, immunohistochemistry as well as quantitative polymerase chain reaction (RT-qPCR) was utilized to ascertain the expression of FRGs. Results. Two molecular subgroups (cluster 1 and cluster 2) with different FRG expression patterns were recognized. A ferroptosis-related model based on 4 genes (VEGFA, CA9, DERL3, and RNF130) was developed through TCGA database to identify the unfavorable prognosis cases. Patients in cluster 1 showed significantly decreased overall survival in contrast with those in cluster 2 ( P < 0.05 ). The LASSO technique and Cox regression analysis were both utilized to establish the independence of the prognostic model. The validity of nomogram prognostic predictions has been well demonstrated for 3- and 5-year survival in both internal and external data validation cohorts. These two subgroups showed striking differences in tumor-infiltrating leukocytes and tumor mutation burden. The low-risk subgroup showed a longer overall survival time with a higher immune cell score and higher tumor mutation rate. Gene functional enrichment analyses revealed predominant enrichment in various tumor-associated signaling pathways. Finally, the expression of each gene was confirmed by immunohistochemistry and RT-qPCR. Conclusion. A novel and comprehensive ferroptosis-related gene model was proposed for cervical cancer which was capable of distinguishing the patients independently with high risk for poor survival, and targeting ferroptosis may represent a promising approach for the treatment of CC.

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Pir2/Rnf144b is a potential endometrial cancer biomarker that promotes cell proliferation

Cited by 14 publications

References 56 publications

Regulation of the p53 Family Proteins by the Ubiquitin Proteasomal Pathway

Regulation of the p53 Family Proteins by the Ubiquitin Proteasomal Pathway

Integrated computational analysis revealed hub genes in lung adenocarcinoma

Systematic Construction and Validation of a Novel Ferroptosis-Related Gene Model for Predicting Prognosis in Cervical Cancer

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