piR-19166 inhibits migration and metastasis through CTTN/MMPs pathway in prostate carcinoma

Qi, Tingyue; Cao, Haiyan; Sun, Hongyan; Feng, Hao; Li, Nianfeng; Wang, Chenghai; Wang, Lei

doi:10.18632/aging.103677

Cited by 13 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, EVs secreted from tumor cells may contain a variety of piRNAs originating from tumor cells, which may be aberrantly expressed in tumor cells [29]. Early evidence showed that piRNAs are involved in PCa development and progression, such as proliferation, apoptosis, metastasis, and invasion [19,20,30]. However, the diagnostic value of piRNA for PCa diagnosis has not been investigated.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of piRNA Targets in Urinary Extracellular Vesicles for the Diagnosis of Prostate Cancer

et al. 2021

View full text Add to dashboard Cite

Emerging studies demonstrate that PIWI-interacting RNAs (piRNAs) are associated with various human cancers. This study aimed to evaluate the urinary extracellular vesicles (EVs) piRNAs as non-invasive biomarkers for prostate cancer (PCa) diagnosis. RNA was extracted from urinary EVs from five PCa patients and five healthy controls (HC), and the piRNAs were analyzed by small RNA sequencing. Dysregulated piRNAs were identified and then validated in another 30 PCa patients and 10 HC by reverse-transcription polymerase chain reaction (RT-qPCR). The expressions of novel_pir349843, novel_pir382289, novel_pir158533, and hsa_piR_002468 in urinary EVs were significantly increased in the PCa group compared with the HC group. The area under the curve (AUC) of novel_pir158533, novel_pir349843, novel_pir382289, hsa_piR_002468, and the combination of the four piRNA in PCa diagnosis was 0.723, 0.757, 0.777, 0.783, and 0.853, respectively. After the RNAhybrid program analysis, all four piRNAs had multiple potential binding sites with key mRNAs in PTEN/PI3K/Akt, Wnt/beta-catenin, or androgen receptor pathway, which are critical in PCa development and progression. In conclusion, our findings indicate that specific piRNAs in urinary EVs may serve as non-invasive diagnostic biomarkers for PCa.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Thus, modulating the expression of piRNAs may reverse these phenotypes. As for PCa, several recent studies revealed that piRNAs were involved in the proliferation, migration, and invasion of PCa cells by conducting different signal pathways, which may represent a new marker of PCa diagnosis [19,20].…”

Section: Introductionmentioning

confidence: 99%

Identification of piRNA Targets in Urinary Extracellular Vesicles for the Diagnosis of Prostate Cancer

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Further, the piRNA-30473/WTAP/HK2 axis promotes the occurrence of breast cancer by regulating the methylation of m6A RNA in diffuse large B-cell lymphoma[ 34 ]. piRNA-19166 inhibits cell migration and metastasis through the cortactin/mitochondrial membrane potential pathway in prostate cancer[ 35 ]. piRNA-823 promotes the angiogenesis of endothelial cells by promoting the secretion of VEGF and interleukin -6.…”

Section: Discussionmentioning

confidence: 99%

Expression and role of P-element-induced wimpy testis-interacting RNA in diabetic-retinopathy in mice

Yu¹,

Ren²,

Chen³

2021

WJD

View full text Add to dashboard Cite

BACKGROUND As one of the major microvascular complications of diabetes, diabetic retinopathy (DR) is the leading cause of blindness in the working age population. Because the extremely complex pathogenesis of DR has not been fully clarified, the occurrence and development of DR is closely related to tissue ischemia and hypoxia and neovascularization The formation of retinal neovascularization (RNV) has great harm to the visual acuity of patients. AIM To investigate the expression of P-element-induced wimpy testis-interacting RNA (piRNA) in proliferative DR mice and select piRNA related to RNV. METHODS One hundred healthy C57BL/6J mice were randomly divided into a normal group as control group (CG) and proliferative DR (PDR) group as experimental group (EG), with 50 mice in each group. Samples were collected from both groups at the same time, and the lesions of mice were evaluated by hematoxylin and eosin staining and retinal blood vessel staining. The retinal tissues were collected for second-generation high-throughput sequencing, and the differentially expressed piRNA between the CG and EG was detected, and polymerase chain reaction (PCR) was conducted for verification. The differentially obtained piRNA target genes and expression profiles were enrichment analysis based on gene annotation (Gene Ontology) and Kyoto Encyclopedia of Genes and Genomes. RESULTS In the CG there was no perfusion area, neovascularization and endothelial nucleus broke through the inner boundary membrane of retinap. In the EG, there were a lot of nonperfused areas, new blood vessels and endothelial nuclei breaking through the inner boundary membrane of the retina. There was a statistically significant difference in the number of vascular endothelial nuclei breaking through the inner retinal membrane between the two groups. High-throughput sequencing analysis showed that compared with the CG, a total of 79 piRNAs were differentially expressed in EG, among which 43 piRNAs were up-regulated and 36 piRNAs were down-regulated. Bioinformatics analysis showed that the differentially expressed piRNAs were mainly concentrated in the signaling pathways of angiogenesis and cell proliferation. Ten piRNAs were selected for PCR, and the results showed that the expression of piR-MMU-40373735, piR-MMU-61121420, piR-MMU-55687822, piR-MMU-1373887 were high, and the expression of piR-MMU-7401535, piR-MMU-4773779, piR-MMU-1304999, and piR-MMU-5160126 were low, which were consistent with the sequencing results. CONCLUSION In the EG, the abnormal expression of piRNA is involved in the pathway of angiogenesis and cell proliferation, suggesting that piRNAs have some regulatory function in proliferative diabetic-retinopathy.

show abstract

“…Transfection of PCa cells by piR_19166 suppressed migration and metastasis via CTTN/matrix metalloproteinases (MMPs) pathway in PCa cells. Authors propose that piR_19166, through regulation of the oncogene CTTN, inhibits migration and distant metastasis of prostate cancer cells and may represent a new marker of diagnosis and treatment for PCa patients in early stages [ 196 ].…”

Section: The Role Of Pirnas In Gcmentioning

confidence: 99%

piRNAs and PIWI Proteins as Diagnostic and Prognostic Markers of Genitourinary Cancers

et al. 2022

View full text Add to dashboard Cite

piRNAs (PIWI-interacting RNAs) are small non-coding RNAs capable of regulation of transposon and gene expression. piRNAs utilise multiple mechanisms to affect gene expression, which makes them potentially more powerful regulators than microRNAs. The mechanisms by which piRNAs regulate transposon and gene expression include DNA methylation, histone modifications, and mRNA degradation. Genitourinary cancers (GC) are a large group of neoplasms that differ by their incidence, clinical course, biology, and prognosis for patients. Regardless of the GC type, metastatic disease remains a key therapeutic challenge, largely affecting patients’ survival rates. Recent studies indicate that piRNAs could serve as potentially useful biomarkers allowing for early cancer detection and therapeutic interventions at the stage of non-advanced tumour, improving patient’s outcomes. Furthermore, studies in prostate cancer show that piRNAs contribute to cancer progression by affecting key oncogenic pathways such as PI3K/AKT. Here, we discuss recent findings on biogenesis, mechanisms of action and the role of piRNAs and the associated PIWI proteins in GC. We also present tools that may be useful for studies on the functioning of piRNAs in cancers.

show abstract

piR-19166 inhibits migration and metastasis through CTTN/MMPs pathway in prostate carcinoma

Cited by 13 publications

References 29 publications

Identification of piRNA Targets in Urinary Extracellular Vesicles for the Diagnosis of Prostate Cancer

Identification of piRNA Targets in Urinary Extracellular Vesicles for the Diagnosis of Prostate Cancer

Expression and role of P-element-induced wimpy testis-interacting RNA in diabetic-retinopathy in mice

piRNAs and PIWI Proteins as Diagnostic and Prognostic Markers of Genitourinary Cancers

Contact Info

Product

Resources

About