Piperine inhibits the proliferation of human prostate cancer cells via induction of cell cycle arrest and autophagy

Ouyang, Dong-Yun; Zeng, Longhui; Pan, Hao; Xu, Lihui; Wang, Yao; Liu, Kun-peng; He, Xian-Hui

doi:10.1016/j.fct.2013.08.007

Cited by 107 publications

(61 citation statements)

References 34 publications

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“…Thus, these two methods could be useful for the evaluation of drug-induced PD models. PIP and PLG both have been shown to confer resistance to oxidative stress (Chonpathompikunlert et al, 2011;Raj et al, 2011;Umar et al, 2013) and have anti-inflammatory (Murunikkara et al, 2012;Lee et al, 2013) and anti-cancer effects (Jarvius et al, 2013;Ouyang et al, 2013). Although the mechanistic bases of these effects are not fully understood, mitochondria could be a potential target.…”

Section: Discussionmentioning

confidence: 98%

Protection effect of piperine and piperlonguminine from Piper longum L. alkaloids against rotenone-induced neuronal injury

et al. 2016

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Section: Discussionmentioning

confidence: 98%

Protection effect of piperine and piperlonguminine from Piper longum L. alkaloids against rotenone-induced neuronal injury

et al. 2016

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“…This suggests that the murine macrophages RAW264.7 cells are more sensitive to piperine than rabbit platelets, but the pharmacological basis of this difference is unclear. Previous studies have demonstrated that the active concentrations of piperine vary, depending on the cell type and animal species in which its effects are assessed [39,40]. Therefore, through further mechanism studies, we evaluated the activity of piperine at concentrations that exhibited inhibitory activity in each of the platelet aggregation assay and RAW264.7 prostaglandin-generation experiments.…”

Section: Discussionmentioning

confidence: 99%

Piperine Inhibits the Activities of Platelet Cytosolic Phospholipase A2 and Thromboxane A2 Synthase without Affecting Cyclooxygenase-1 Activity: Different Mechanisms of Action Are Involved in the Inhibition of Platelet Aggregation and Macrophage Inflammatory Response

et al. 2014

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PURPOSE: Piperine, a major alkaloid of black pepper (Piper nigrum) and long pepper (Piper longum), was shown to have anti-inflammatory activity through the suppression of cyclooxygenase (COX)-2 gene expression and enzyme activity. It is also reported to exhibit anti-platelet activity, but the mechanism underlying this action remains unknown. In this study, we investigated a putative anti-platelet aggregation mechanism involving arachidonic acid (AA) metabolism and how this compares with the mechanism by which it inhibits macrophage inflammatory responses; METHODS: Rabbit platelets and murine macrophage RAW264.7 cells were treated with piperine, and the effect of piperine on the activity of AA-metabolizing enzymes, including cytosolic phospholipase A2 (cPLA2), COX-1, COX-2, and thromboxane A2 (TXA2) synthase, as well as its effect on AA liberation from the plasma membrane components, were assessed using isotopic labeling methods and enzyme immunoassay kit; RESULTS: Piperine significantly suppressed AA liberation by attenuating cPLA2 activity in collagen-stimulated platelets. It also significantly inhibited the activity of TXA2 synthase, but not of COX-1, in platelets. These results suggest that piperine inhibits platelet aggregation by attenuating cPLA2 and TXA2 synthase activities, rather than through the inhibition of COX-1 activity. On the other hand, piperine significantly inhibited lipopolysaccharide-induced generation of prostaglandin (PG)E2 and PGD2 in RAW264.7 cells by suppressing the activity of COX-2, without effect on cPLA2; CONCLUSION: Our findings indicate that piperine inhibits platelet aggregation and macrophage inflammatory response by different mechanisms.

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“…GBOT can inhibit the peroxidation products induced by the carcinogenic substances in the intestinal mucosa and increase the activity of glutathione (GSH) and Na-K-ATP enzyme. Antioxidation of GBOT adjusts oxidation changes by inhibiting lipid peroxidation and indirectly increasing synthesis and conduction of GSH (Ouyang et al, 2013). Oxidative stress models of rats fed high-fat diet received GBOT.…”

Section: Discussionmentioning

confidence: 99%

Effect of GBOT on blood lipid and blood glucose metabolism in rats with atherosclerosis

2015

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ABSTRACT. We observed the variation in in vivo blood lipid and blood glucose metabolism in rats with atherosclerosis after 5-(3,4-dihydroxyphenyl)-1-piperidin-1-yl-penta-2,4-dien-1-one (GBOT) administration. Wistar rats aged 10 weeks received a high-fat diet to establish the atherosclerosis model. Metabolic indices related to blood lipid and blood glucose were measured before modeling and at 4 and 8 weeks after modeling. Liver fat levels in rats were measured at 8 weeks to analyze the relationship between liver fat and blood lipid levels. We examined the mechanism of blood lipid reduction. The levels of serum triglycerides, total cholesterol, and very-low-density lipoprotein cholesterol in rats in the control group were significantly decreased (P < 0.05) compared with those in the 4-week control group at 4 weeks and decreased significantly and continuously until the 8th week (P < 0.05). Compared with the 8-week control group, the blood glucose level in rats in the 8-week experimental group decreased significantly (P < 0.05), and the level of insulin sensitivity index decreased significantly (P < 0.05). Compared with the control group, triglyceride and total cholesterol levels per unit mass in rat liver tissue in the 8-week experimental group decreased significantly (P < 0.05). Western blotting indicated that GBOT significantly increased the expression of lecithin-cholesterol acyltransferase, low-density lipoprotein receptor, and cholesterol 7 alpha-hydroxylase proteins. GBOT can significantly decrease the levels of blood lipid and blood glucose in rat models of atherosclerosis, and its mechanism may be associated with the promotion of expression of lecithin-cholesterol acyltransferase, low-density lipoprotein receptor, and cholesterol 7 alpha-hydroxylase proteins.

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Piperine inhibits the proliferation of human prostate cancer cells via induction of cell cycle arrest and autophagy

Cited by 107 publications

References 34 publications

Protection effect of piperine and piperlonguminine from Piper longum L. alkaloids against rotenone-induced neuronal injury

Protection effect of piperine and piperlonguminine from Piper longum L. alkaloids against rotenone-induced neuronal injury

Piperine Inhibits the Activities of Platelet Cytosolic Phospholipase A2 and Thromboxane A2 Synthase without Affecting Cyclooxygenase-1 Activity: Different Mechanisms of Action Are Involved in the Inhibition of Platelet Aggregation and Macrophage Inflammatory Response

Effect of GBOT on blood lipid and blood glucose metabolism in rats with atherosclerosis

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