Piperine Inhibits Lipopolysaccharide‐induced Maturation of Bone‐marrow‐derived Dendritic Cells Through Inhibition of ERK and JNK Activation

Abstract

Piperine, one of the main components of Piper longum Linn. and P. nigrum Linn., is a plant alkaloid with a long history of medicinal use. Piperine has been shown to modulate the immune response, but the mechanism underlying this modulation remains unknown. Here, we examined the effects of piperine on lipopolysaccharide (LPS)-induced inflammatory responses in bone-marrow-derived dendritic cells (BMDCs). Piperine significantly inhibited the expression of major histocompatibility complex class II, CD40 and CD86 in BMDCs in a dose-dependent manner. Furthermore, piperine treatment led to an increase in fluorescein-isothiocyanate-dextran uptake in LPS-treated dendritic cells and inhibited the production of tumour necrosis factor alpha and interleukin (IL)-12, but not IL-6. The inhibitory effects of piperine were mediated via suppression of extracellular signal-regulated kinases and c-Jun N-terminal kinases activation, but not p38 or nuclear factor-κB activation. These findings provide insight into the immunopharmacological role of piperine.

Piperine inhibit inflammation, alveolar bone loss and collagen fibers breakdown in a rat periodontitis model

“…It has been used as a medicinal herb for the treatment of rheumatoid arthritis in Japan, Korea, China and India (16). Piperine inhibited ERK and JNK pathways in dendritic cells, which supports its reputed use against some immune diseases such as arthritis and diabetes (17). It also possesses a broad-spectrum antimicrobial activity (18).…”

Piperine blocks interleukin-2-driven cell cycle progression in CTLL-2 T lymphocytes by inhibiting multiple signal transduction pathways

“…The ability of piperine to inhibit inflammation has been attributed to a reduction in inflammatory mediator expression by macrophages in the presence of piperine Ying et al, 2013). However, piperine also inhibits lipopolysaccharide-induced maturation of dendritic cells, suggesting a decreased capacity to present antigen to T lymphocytes (Bae et al, 2012). In addition, piperine suppresses mouse T lymphocyte activation by mitogens (Dogra et al, 2004) and inhibits synthesis of Th2 cytokines in a mouse model of asthma (Kim and Lee, 2009).…”

Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro

“…In contrast, Piper nigrum Linn. extract, piperine, significantly inhibited the maturation (MHC class II, CD40 and CD86), cytokine production (TNF-α and IL-12), phosphorylation of ERK and JNK, but enhanced the endocytosis activity of LPS-induced bone-marrow-derived DCs [32]. However, we did not observe significant alteration in the maturation markers of both conditions of treated mDCs.…”

“…The other plant components in AVS073 also have anti-inflammatory effect and analgesic activity [24–30]. Some components of AVS073 showed direct anti-cancer properties through inhibiting cell growth or inducing cellular apoptosis, and indirect pathway via the immunological action of immune cells [31, 32]. Several in vitro and in vivo studies demonstrated that the extracts of AVS073′s components carried antioxidant, anti-inflammatory, immunomodulating and anti-cancer actions [33–40].…”

“…Furthermore, Carthamus tinctorius L. treated DCs maintained the high profile of maturation markers in tumor antigen pulsed-DCs [31]. In contrast, Piper nigrum L. significantly inhibited the phenotypic maturation, cytokine production (TNF-α and IL-12), phosphorylation of ERK and JNK, but enhanced the endocytosis activity of LPS-induced bone-marrow-derived DCs [32]. DCs, which can subsequently interact with CIK cells [49, 51], might be potential targets of this recipe.…”