Piperine Augments the Protective Effect of Curcumin Against Lipopolysaccharide-Induced Neurobehavioral and Neurochemical Deficits in Mice

Jangra, Ashok; Kwatra, Mohit; Singh, Tavleen; Pant, Rajat; Kushwah, Pawan; Sharma, Yash; Saroha, Babita; Datusalia, Ashok Kumar; Bezbaruah, Babul Kumar

doi:10.1007/s10753-016-0332-4

Cited by 33 publications

(27 citation statements)

References 81 publications

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“…Harish et al exposed curcumin-pretreated N27 cells to buthionine sulfoximine and observed the upregulation of GSH and glutathione S-transferase [22]. The effect on GSH levels was also observed in a lipopolysaccharide-induced mouse model with curcumin treatment for seven days [23]. Consistently, we also observed an upregulation of SOD and GSH levels, suggesting that curcumin could regulate oxidative homeostasis in HUVECs by stimulating the activities of antioxidant enzymes.…”

Section: Discussionsupporting

confidence: 85%

Curcumin Protects Human Umbilical Vein Endothelial Cells against H₂O₂-Induced Cell Injury

Ouyang

Shi

et al. 2019

Pain Research and Management

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Migraine is a prevalent neurological disorder which causes a huge economic burden on society. It is thought to be a neurovascular disease with oxidative stress might be involved. Curcumin, one of the major ingredients of turmeric, has potent antioxidative and anti-inflammatory properties, but whether it could be used as a potential treatment for migraine remains to be explored. In the present study, human umbilical vein endothelial cells (HUVECs) were pretreated with various concentrations of curcumin (0 μM, 10 μM, 20 μM, 30 μM, 40 μM, and 50 μM) for 12 h, thereby exposed to H2O2 (100 μM) for another 12 h. The viability of HUVECs was tested by the CCK-8 assay, and the activities of antioxidant enzymes including superoxide dismutase (SOD) and glutathione (GSH) were also examined. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were assayed to determine H2O2-induced oxidative stress. In addition, several cell death-related genes (p53, p21, Bax, and Bcl-2) were detected by PCR, and an apoptosis-related protein (caspase3) was evaluated by western blotting. Our results showed that curcumin improved the H2O2-induced decrease of cell viability and antioxidative enzyme activities and decreased the level of oxidative stress. As a conclusion, curcumin could mitigate H2O2-induced oxidative stress and cell death in HUVECs and may be a potential therapeutic drug for migraine.

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Section: Discussionsupporting

confidence: 85%

Curcumin Protects Human Umbilical Vein Endothelial Cells against H₂O₂-Induced Cell Injury

Ouyang

Shi

et al. 2019

Pain Research and Management

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“…Curcumin is one of the most frequently studied natural products to date, due to its broad-spectrum preclinical activity and low oral toxicity. Curcumin is reported to be a beneficial treatment for inflammation in patients [13] by exerting protective effects on the nervous system [19], circulatory system, digestive system, and tumor metastasis [20]. In patients with OA, curcumin improves quality of life and enables a decrease in the consumption of steroidal inflammatory drugs [21].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the articular cartilage in the knees of rats with induced arthritis treated with curcumin

2020

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This study was designed to evaluate the anti-inflammatory effects of a curcumin treatment on the knee of rats with induced osteoarthritis. Fifteen adult rats were used and divided in three groups: the osteoarthritis group (OAG), control group (CG-without induction of osteoarthritis), and curcumin-treated osteoarthritis group (COAG). Osteoarthritis was induced in the right knee of rats in the OAG and COAG by administering an intra-articular injection of 1 mg of zymosan. Fourteen days after induction, 50 mg/kg curcumin was administered by gavage daily for 60 days to the COAG. After the treatment period, rats from all groups were euthanized. Medial femoral condyles were collected for light microscopy and immunohistochemical staining. The expression of SOX-5, IHH, MMP-8, MMP-13, and collagen 2 (Col2) was analyzed. The COAG exhibited an increase in the number of chondrocytes in the surface and middle layers compared with that of the OAG and CG, respectively. The COAG also showed a decrease in the thicknesses of the middle and deep layers compared with those of the OAG, and an increase in Col2 expression was observed in all articular layers (surface, middle, and deep) in the COAG compared with that in the OAG. SOX-5 expression was increased in the surface and deep layers of the COAG compared with those in the OAG and CG. Based on the results of this study, the curcumin treatment appeared to exert a protective effect on cartilage, as it did not result in an increase in cartilage thickness or in MMP-8 and MMP-13 expression but led to increased IHH, Col2, and SOX-5 expression and the number of chondrocytes.

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“…Curcumin has been considered as a multifunctional agent and proved to have protective effects on nervous system, 16) circulatory system, 17) digestive system, 18,19) and tumor metastasis. 20,21) Given those factors, we assume that curcumin might also have effect on chondrocyte metabolism.…”

Section: Discussionmentioning

confidence: 99%

Curcumin Inhibits Chondrocyte Hypertrophy of Mesenchymal Stem Cells through IHH and Notch Signaling Pathways

Cao

Dou

Dong

2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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Using tissue engineering technique to repair cartilage damage caused by osteoarthritis is a promising strategy. However, the regenerated tissue usually is fibrous cartilage, which has poor mechanical characteristics compared to hyaline cartilage. Chondrocyte hypertrophy plays an important role in this process. Thus, it is very important to find out a suitable way to maintain the phenotype of chondrocytes and inhibit chondrocyte hypertrophy. Curcumin deriving from turmeric was reported with anti-inflammatory and antitumor pharmacological effects. However, the role of curcumin in metabolism of chondrocytes, especially in the chondrocyte hypertrophy remains unclear. Mesenchymal stem cells (MSCs) are widely used in cartilage tissue engineering as seed cells. So we investigated the effect of curcumin on chondrogenesis and chondrocyte hypertrophy in MSCs through examination of cell viability, glycosaminoglycan synthesis and specific gene expression. We found curcumin had no effect on expression of chondrogenic markers including Sox9 and Col2a1 while hypertrophic markers including Runx2 and Col10a1 were down-regulated. Further exploration showed that curcumin inhibited chondrocyte hypertrophy through Indian hedgehog homolog (IHH) and Notch signalings. Our results indicated curcumin was a potential agent in modulating cartilage homeostasis and maintaining chondrocyte phenotype.Key words curcumin; mesenchymal stem cell; chondrocyte hypertrophy; Indian hedgehog homolog; neurogenic locus notch homolog Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and disability.1) Seventy percent of the population older than 65 years is suffering from varying degrees of OA.2)The pathogenesis of cartilage injury caused by OA is the disruption of the homeostasis between catabolism and anabolism of extracellular matrix.3) However, limited by the intrinsic regeneration capacity of cartilage tissue, repairing damaged articular cartilage currently is a significant challenge. 4,5) With the advances in materials science, cell biology, biomechanics, using tissue engineering technology to construct cartilage provides a novel method for repairing damaged cartilage.6) Mesenchymal stem cells (MSCs) have potential of differentiation into chondrocytes and have got the priority in the seed cells choosing list because of their traits in rich resources, easy to get and low immunogenicity. 7,8) However, the regenerated tissue-engineered cartilage usually is fibrous cartilage rather than hyaline cartilage.9) The chondrocytes in fibrous cartilage proceed in hypertrophic differentiation and secret Col10a1 and matrix metalloproteinase 13 (Mmp13).10) Therefore, suppressing chondrocyte hypertrophy is the key issue to build a stable hyaline cartilage.Curcumin is effective substance of turmeric and possesses various pharmacological effects, including antioxidant, anti-bacterial, anti-tumor and anti-inflammatory.11-13) A recent study has reported that curcumin may be a potential therapeutic candidate for Helicobacter pylori associated diseas...

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Piperine Augments the Protective Effect of Curcumin Against Lipopolysaccharide-Induced Neurobehavioral and Neurochemical Deficits in Mice

Cited by 33 publications

References 81 publications

Curcumin Protects Human Umbilical Vein Endothelial Cells against H₂O₂-Induced Cell Injury

Curcumin Protects Human Umbilical Vein Endothelial Cells against H₂O₂-Induced Cell Injury

Evaluation of the articular cartilage in the knees of rats with induced arthritis treated with curcumin

Curcumin Inhibits Chondrocyte Hypertrophy of Mesenchymal Stem Cells through IHH and Notch Signaling Pathways

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Piperine Augments the Protective Effect of Curcumin Against Lipopolysaccharide-Induced Neurobehavioral and Neurochemical Deficits in Mice

Cited by 33 publications

References 81 publications

Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury

Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury

Evaluation of the articular cartilage in the knees of rats with induced arthritis treated with curcumin

Curcumin Inhibits Chondrocyte Hypertrophy of Mesenchymal Stem Cells through IHH and Notch Signaling Pathways

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Product

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Curcumin Protects Human Umbilical Vein Endothelial Cells against H₂O₂-Induced Cell Injury

Curcumin Protects Human Umbilical Vein Endothelial Cells against H₂O₂-Induced Cell Injury