Piper nigrum ethanolic extract rich in piperamides causes ROS overproduction, oxidative damage in DNA leading to cell cycle arrest and apoptosis in cancer cells

Grinevicius, Valdelúcia M.A.S.; Kviecinski, Maicon Roberto; Mota, Nádia S.R.S.; Ourique, Fabiana; Castro, Luiza Sheyla Evenni P. Will; Andreguetti, Rafaela Rafognato; Correia, João Francisco Gomes; Filho, Danilo Wilhem; Pich, Claus Tröger; Pedrosa, Rozangela Curi

doi:10.1016/j.jep.2016.05.020

Cited by 55 publications

(32 citation statements)

References 42 publications

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“…Appropriate ROS can promote macrophages to play their phagocytosis and enzymolysis immunophysiological functions (Jia et al., 2018). However, the generation of excessive ROS causes apoptosis (Grinevicius et al., 2016). Hence, we utilized the fluorescent probe DCFH‐DA to monitor the production of intracellular ROS.…”

Section: Resultsmentioning

confidence: 99%

Ultrasound‐assisted enzymatic extraction of anthocyanins from grape skins: optimization, identification, and antitumor activity

Tan

Xue

et al. 2020

Journal of Food Science

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Grape skins produced during the grape juice production and processing contain abundant anthocyanins and other active compounds. Consequently, this study optimized the extraction conditions for ultrasound‐assisted enzymatic extraction (UAEE) of anthocyanins from grape skins via response surface methodology coupled with genetic algorithm. The optimum extraction parameters to achieve the highest anthocyanins yield (3.01 ± 0.04) mg/g from grape skins by UAEE were obtained under an extraction temperature of 50 °C, ultrasonic power of 400 W, pectinase dosage of 0.16%, and extraction time of 28 min. The AB‐8 macroporous resin combined Sephadex LH‐20 techniques were further employed to purify the anthocyanins extracts obtained under optimum extraction conditions (AEOEC), and the main anthocyanins were identified using high‐performance liquid chromatography tandem mass spectrometry. The purified anthocyanins contained two anthocyanins in terms of delphinidin‐3,5‐O‐diglucoside and cyanidin‐3‐O‐rutinoside with purity of 91.35% and 92.64%, respectively. Ultimately, we further evaluated the antitumor activity of AEOEC and two purified anthocyanins on breast cancer. The results indicated that the antitumor effect of AEOEC on breast cancer MCF‐7 cells was better than that of two purified anthocyanins. In addition, AEOEC could memorably increase intracellular reactive oxygen species levels and apoptosis of MCF‐7 cells, and arrest MCF‐7 cells in the G2/M phases. The findings provide an effective and feasible method for anthocyanins extraction and reduce the environmental burden of this waste.

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Section: Resultsmentioning

confidence: 99%

Ultrasound‐assisted enzymatic extraction of anthocyanins from grape skins: optimization, identification, and antitumor activity

Tan

Xue

et al. 2020

Journal of Food Science

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“…This is the first report that the TRPV1‐dependent increase in ROS generation and the intracellular Ca 2+ level are involved in piperine‐promoted glucose uptake accompanied by phosphorylation of AMPK in the skeletal muscle cells. Previously, ROS generation by piperine had only been reported in the cancer cell line, human rectal adenocarcinoma HRT‐18 …”

Section: Discussionmentioning

confidence: 99%

Piperine Promotes Glucose Uptake through ROS‐Dependent Activation of the CAMKK/AMPK Signaling Pathway in Skeletal Muscle

Maeda

Shirao

Shirasaya

et al. 2018

Molecular Nutrition Food Res

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“…Aloe-emodin in aloe vera, 6-gingerol in ginger and piperine in black pepper are all reported to inhibit tumor invasion and metastasis through the suppression of expression of matrix metalloproteinase-2/9 (38,42). All ingredients of BU, except epsom salts, have been revealed to exert apoptotic activity in various cancer cell lines, including SMMC-7721 (56), a human glioblastoma cell line (U87MG) (57), prostate cancer (58), H460 non-small cell lung carcinoma (59), adriamycin-resistant human leukemia (K562/ADR) (60), MCF-7 (61), IOMM-Lee and CH157MN (62) via the overexpression of BCL2 associated X, apoptosis regulator (60)(61)(62), caspase 3 (57,62), caspase 8 (57), caspase 9 (59), induced ROS accumulation (56,61) or via the AKT/mitogen-activated protein kinase pathway (60).…”

Section: Discussionmentioning

confidence: 99%

Anti‑angiogenic and anti‑proliferative effects of Benja‑ummarit extract in rats with hepatocellular carcinoma

et al. 2020

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The herbal extract Benja-ummarit (BU) is a traditional Thai medicine with a putative cancer-suppressing effect. However, this effect has only been tested in vitro in human hepatocarcinoma cell lines. The present study determined the efficacy of a BU extract to treat hepatocellular carcinoma (HCC) in rats in vivo and established its anti-angiogenic and anti-proliferative properties. The BU extract was prepared in 95% ethanol and its composition determined using liquid chromatography-mass spectrometry. HCC was induced in Wistar rats by an injection of diethylnitrosamine (DEN), followed 2 weeks later by injections of thioacetamide (TAA) thrice weekly for 4 weeks. Following 2 months, the DEN-TAA-treated rats were divided into 6 groups that were treated orally for another 2 months with: i) No treatment; ii) vehicle; iii) 30 mg/kg sorafenib (SF); iv) 1 mg/kg BU; v) 10 mg/kg BU; or vi) 50 mg/kg BU. Liver samples were collected for gross morphological, histological, reverse transcription-quantitative PCR and western blot analyses, and serum samples were collected for liver function tests. The size and number of the cancer nodules were reduced ~10-fold in BU-treated HCC groups and ~14-fold in the SF-treated group compared with the HCC group. Furthermore, the serum parameters of liver damage were lower in BU-compared with SF-treated rats. These results indicate that while each of these formulations strongly reduce HCC expansion, BU extract results in less liver damage. Vascular endothelial growth factor expression was reduced significantly in the BU-and SF-treated HCC groups compared with the HCC group (P<0.05). BU extract antagonizes HCC growth in vivo potently through inhibiting tumor angiogenesis. BU, therefore, qualifies as a promising medical herb requiring further evaluation as a treatment of HCC.

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Piper nigrum ethanolic extract rich in piperamides causes ROS overproduction, oxidative damage in DNA leading to cell cycle arrest and apoptosis in cancer cells

Cited by 55 publications

References 42 publications

Ultrasound‐assisted enzymatic extraction of anthocyanins from grape skins: optimization, identification, and antitumor activity

Ultrasound‐assisted enzymatic extraction of anthocyanins from grape skins: optimization, identification, and antitumor activity

Piperine Promotes Glucose Uptake through ROS‐Dependent Activation of the CAMKK/AMPK Signaling Pathway in Skeletal Muscle

Anti‑angiogenic and anti‑proliferative effects of Benja‑ummarit extract in rats with hepatocellular carcinoma

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