Piper guineense Leaf Extract Elevates Serum Follicle Stimulating Hormone Level in the Diestrus Phase in Non-Pregnant Female Albino Wistar Rats

Agbai, E O; Onyebuagu, P. C.; Njoku, C. J.; Jervas, Ekezie; Eke, C. C.; Nwanegwo, C O; Nwafor, A

doi:10.9734/jocamr/2017/31664

Cited by 3 publications

(6 citation statements)

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“…There could be possibility of extraovarian progesterone constituting to the serum progesterone pool. In Piper guineense extract treated Group C rats, FSH remained normal whereas LH was significantly reduced in contrast with the results of our recent study that showed that the extract caused increased FSH levels and LH peak during the diestrus phase of the estrous cycle [11] . Although the present study evaluated serum FSH and LH levels irrespective of the estrous cycle, the differences in serum FSH and LH levels could be dependent on the estrous cycle since the rats received the same doses of Piper guineense leaf extract (200 mgkg ).…”

Section: Effect Of Ibuprofen On the Serum Fsh Lh And Estrogen Levelcontrasting

confidence: 99%

“…In Southern Nigeria, Piper guineense seed and leaf are used in the preparation of postpartum tonics to enhance expulsion of debris although study has shown that the extract enhances uterine muscle contraction in order to expel debris postpartum [9] . Because the leaves are locally used for female infertility [10] led to our recent study that showed Piper guineense leaf extract caused elevation of folliclestimulating hormone (FSH) level during diestrus phase of estrous cycle [11] . Moreover, increased serum FSH levels has been implicated in impairment of ovarian function associated with decreased number of follicles [12] .…”

Section: Introductionmentioning

confidence: 99%

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Comparison of inhibitory effect of ibuprofen with Piper guineense Schumach and Thonn. on some reproductive hormones in female rats

Agbai¹,

Eke²,

Nwanegwo³

et al. 2017

J Phytopharmacol

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The aim of the present study was to compare the inhibitory effect of ibuprofen with oral administration of Piper guineense leaf extract on follicle-stimulating hormone, luteinizing hormone, progesterone and estrogen in female rats irrespective of the estrous cycle. The animals were randomly assigned to four groups (n = 7): group A (control), Group B, 180 mg-kg of ibuprofen, Group C, 200 mg-kg of Piper guineense extract, Group D, 180 mg-kg of ibuprofen and 200 mg-kg of Piper guineense extract. At the end of two weeks administration, rats were sacrificed under urethane anesthesia and hormones measured using enzyme-linked immunosorbent assay method. Results showed significant reduction in serum follicle-stimulating hormone and luteinizing hormone following ibuprofen administration in Group B rats at P < 0.05. Piper guineense extract treated Group C rats caused significant reduction in serum luteinizing hormone and progesterone at P < 0.05. In contrast, serum follicle stimulating hormone significantly increased in Group D rats at P < 0.05 whereas serum luteinizing hormone and progesterone were markedly reduced at P < 0.05. Serum estrogen level remained unchanged among groups. In conclusion, results obtained suggested that extract inhibited luteinization of follicles thus could impair ovulation, therefore the extract can be used as oral contraceptive in family planning.

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Section: Effect Of Ibuprofen On the Serum Fsh Lh And Estrogen Levelcontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of inhibitory effect of ibuprofen with Piper guineense Schumach and Thonn. on some reproductive hormones in female rats

Agbai¹,

Eke²,

Nwanegwo³

et al. 2017

J Phytopharmacol

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“…Agbai. [9] also reported that the aqueous extract ofP.guineese leaf was capable of influencing follicle stimulating hormone production during dietrus phase without affecting the luteinizing hormone and estrogen levels. Therefore haltering the early follicular phase which resulted to a decreased population of follicles in the ovary.…”

Section: Hyperlipidemiamentioning

confidence: 96%

The Effect of Nigella sativa and Piper guineense Aqueous Extracts on the in Vivo Expression Profile of Lipid and Sex Hormones

Franca

Rume

2019

BJI

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Aim: The study investigated the effect of aqueous extract of Piper guineense and Nigella sativa on Testosterone, Estrogen and Follicle stimulating hormone levels in male and female rats induced by diets supplemented with high dose of sucrose and margarine for four (4) week. Materials and Methods: Thirty male and female Wistar rats of average weight (100 g) were used for the study. The rats were arranged into five groups with six rats in each of the groups. The rats had access to their normal feed but sucrose and margarine were used to induce hyperglycemia and hyperlipidemia respectively on the rats with the exception of the rats in the positive control group. The rats in the negative control were induced using the sucrose and margarine but were not treated using the aqueous extracts. The rats in the Piper guineense group were treated with 2 ml of Piper guineense aqueous leaf extract, while the rats in the Nigella sativa group were treated with 2 ml of Nigella sativa aqueous extract. The rats in the Nigella sativa and Piper guineense group were treated with 2 ml of the combined aqueous extract. Results: The results showed that the extracts had an increasing effect which was time dependent on the hormones. The highest increase was obtained on the third week of feeding when compared to the control. The testosterone levels (mlU/ml) showed for the, positive control (2.28 ± 0.08), uziza leaf (6.45 ± 0.01), black seed (2.85 ± 0.01), black seed &uziza (8.47 ± 0.08)(p<0.05). The increase for estrogen levels (mlU/ml) showed for the positive control (99 ± 0.82), uziza leaf (98 ± 1.63), black seed (100 ± 1.63), black seed & uziza (113 ± 0.82). Furthermore, the extracts also had an increasing effect on the follicle stimulating hormone levels with the highest increase obtained on the third week (p>0.05). The FSH levels (mlU/ml)) showed for the positive control (0.23 ± 0.01), uziza leaf 0.25 ± 0.01), black seed (0.54 ± 0.02), black seed & uziza (0.58 ± 0.01)(p<0.05). Histopathological findings also suggest that treatment with the aqueous extracts of P.guineense and N .sativa after inducement salvaged the testes and ovaries when compared to the induced group after the second week of administration. Although the combined mixture N.sativa and P.guineense extract was more effective. Conclusion: The results of the study indicate that the aqueous extract of a combined mixture of Nigella sativa and Piper guineense might be an effective plant in indigenous medicine, which may be used to combat infertility caused by hormonal imbalance.

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“…These herbal plants alter reproductive hormones, therefore, disrupt the reproductive cycle. Recent studies have demonstrated that Tetrapleura tetraptera and Piper guineense altered luteinizing hormone secretion during proestrus phase of the estrous cycle in rats [16,17]. Meanwhile, in ancient times, women drank extracted water from Xylopia aethiopica as a natural method of contraception that became part of Igbo folklore and this led to the present research work.…”

Section: Introductionmentioning

confidence: 92%

“…In Africa, traditional methods of contraception are on the increase and have been increasingly sought for in herbal plants because herbs are effective, cheap and safe [11] [16,17]. Meanwhile, in ancient times, women drank extracted water from Xylopia aethiopica as a natural method of contraception that became part of Igbo folklore and this led to the present research work.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study between Xylopia aethiopica Dried Fruit Extract and Ibuprofen Inhibiting Effects on Some Reproductive Hormones Irrespective of the Estrous Cycle

Onuka

Okechukwu

Maxine

2017

IJCAM

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Ibuprofen is often used for contraception, therefore, Xylopia aethiopica dried fruit extract and ibuprofen were tested on follicle-stimulating hormone (FSH), luteinizing hormone (LH), glucocorticoid, progesterone and estrogen levels in adult female rats in order to determine whether Xylopia aethiopica dried fruit extract can be used for contraception. Twenty one rats were randomly divided into 3 groups (n = 7) comprising of control group A, experimental Group B and C. The experimental Group B received 180 mg/kg of ibuprofen daily and experimental Group C received 300 mg/kg of Xylopia aethiopica dried fruit extract. All administration of drug and extract was done via oral route and lasted for 14 days independent of estrous cycle. Using Enzyme-linked Immunosorbent Assay the serum FSH, LH, glucocorticoid, progesterone and estrogen in both extract and ibuprofen treated rats were measured. Results showed that ibuprofen caused significant reduction of FSH (P<0.05). The extract did not cause significant difference in the serum FSH level. Both ibuprofen and extract caused significant reduction (P<0.05) in the serum LH levels. Both ibuprofen and extract caused significant elevation (P< 0.05) in glucocorticoid level. However, the extract caused significant reduction (P<0.05) in the serum progesterone and estrogen levels respectively compared to ibuprofen. In that sense, Xylopia aethiopica dried fruit extract acted as a more potent contraceptive, inhibitor of ovulatory hormones than ibuprofen. and selectively blocks estrogen-mediated receptors in rats [7]. Moreover, the use of synthetic steroidal contraceptives has proven unsafe to the health of the user as they pose risk of side effects [8][9][10]. Keywords:In Africa, traditional methods of contraception are on the increase and have been increasingly sought for in herbal plants because herbs are effective, cheap and safe [11] [16,17]. Meanwhile, in ancient times, women drank extracted water from Xylopia aethiopica as a natural method of contraception that became part of Igbo folklore and this led to the present research work. The plant, Xylopia aethiopica commonly known as "Uda" in Igbo language, "Eeru alamo" in Yoruba language and "Chimba" in Hausa language is a tropical evergreen, aromatic plant that grows commonly in wet swampy soils and forest zones of West Africa characterized with a slim tall tree of about 15 -30 m tall and 0.6 -0.7 m in diameter, straight stem, slightly stripped or smooth bark with consistent pepperish aroma with dry brown, corrugated in shape with pods containing 4 -9 kidney shaped peppery seeds about 0.05 m in length [18,19].However, antifertility effect of Xylopia aethiopica have been demonstrated in male rats [20,21]. In another study unpublished, we demonstrated that Xylopia aethiopica dried fruit extract caused alteration in luteinizing hormone (LH), follicle-stimulating hormone (FSH), and glucocorticoid hormone during proestrus phase of clomiphene-treated rats. Because Xylopia aethiopica have analgesic properties and can be used in t...

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Piper guineense Leaf Extract Elevates Serum Follicle Stimulating Hormone Level in the Diestrus Phase in Non-Pregnant Female Albino Wistar Rats

Cited by 3 publications

References 0 publications

Comparison of inhibitory effect of ibuprofen with Piper guineense Schumach and Thonn. on some reproductive hormones in female rats

Comparison of inhibitory effect of ibuprofen with Piper guineense Schumach and Thonn. on some reproductive hormones in female rats

The Effect of Nigella sativa and Piper guineense Aqueous Extracts on the in Vivo Expression Profile of Lipid and Sex Hormones

A Comparative Study between Xylopia aethiopica Dried Fruit Extract and Ibuprofen Inhibiting Effects on Some Reproductive Hormones Irrespective of the Estrous Cycle

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