Pioneering Effects and Enhanced Neurite Complexity of Primary Hippocampal Neurons on Hierarchical Neurotemplated Scaffolds

Kim, Beom Jin; Park, Matthew; Park, Ji Hun; Joo, Su-Chong; Kim, Mi Hee; Kang, Kyung‐Tae; Choi, Insung S.

doi:10.1002/adhm.201800289

Cited by 5 publications

(3 citation statements)

References 42 publications

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“…In addition, the surface microstructure of biomaterials also affects the phenotype of neurons. A neuro-templated scaffold was developed from silicification and calcination of the 2D cultured neural network, and neurons cultured on this biologically inert neuro-templated topography have significantly elongated axons ( Kim B. J. et al, 2018 ). More simplified designs, such as polydimethylsiloxane (PDMS) scaffolds with dense micropillars on the surface, can also promote the maturation of neurons and be used for the construction of brain injury models ( Chen C. et al, 2019 ).…”

Section: Scaffold-based Modelmentioning

confidence: 99%

Recent progresses in novel in vitro models of primary neurons: A biomaterial perspective

Zhang

Yang

et al. 2022

Front. Bioeng. Biotechnol.

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Central nervous system (CNS) diseases have been a growing threat to the health of humanity, emphasizing the urgent need of exploring the pathogenesis and therapeutic approaches of various CNS diseases. Primary neurons are directly obtained from animals or humans, which have wide applications including disease modeling, mechanism exploration and drug development. However, traditional two-dimensional (2D) monoculture cannot resemble the native microenvironment of CNS. With the increasing understanding of the complexity of the CNS and the remarkable development of novel biomaterials, in vitro models have experienced great innovation from 2D monoculture toward three-dimensional (3D) multicellular culture. The scope of this review includes the progress of various in vitro models of primary neurons in recent years to provide a holistic view of the modalities and applications of primary neuron models and how they have been connected with the revolution of biofabrication techniques. Special attention has been paid to the interaction between primary neurons and biomaterials. First, a brief introduction on the history of CNS modeling and primary neuron culture was conducted. Next, detailed progress in novel in vitro models were discussed ranging from 2D culture, ex vivo model, spheroid, scaffold-based model, 3D bioprinting model, and microfluidic chip. Modalities, applications, advantages, and limitations of the aforementioned models were described separately. Finally, we explored future prospects, providing new insights into how basic science research methodologies have advanced our understanding of the CNS, and highlighted some future directions of primary neuron culture in the next few decades.

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Section: Scaffold-based Modelmentioning

confidence: 99%

Recent progresses in novel in vitro models of primary neurons: A biomaterial perspective

Zhang

Yang

et al. 2022

Front. Bioeng. Biotechnol.

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“…The 2D terrain is a general term for various micropatterns of the matrix surface ( Zhang et al, 2018 ). Nerve cells in contact with the surface micropatterns of the matrix are subjected to pressure that is asymmetric and irregular compared to the flat surface, thus initiating the process of converting mechanical stimuli into biochemical signals that influence nerve cell behavior ( Jeon et al, 2014 ; Kim et al, 2018 ). Advances in manufacturing technology have resulted in a number of 2D terrains designed to guide the behavior of nerve cells, such as nerve templates, honeycombs, oriented fiber arrangements, and grooves.…”

Section: Regulation Of Physical Cues On Neuronal Behaviormentioning

confidence: 99%

Physical Cues of Matrices Reeducate Nerve Cells

Luo

et al. 2021

Front. Cell Dev. Biol.

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The behavior of nerve cells plays a crucial role in nerve regeneration. The mechanical, topographical, and electrical microenvironment surrounding nerve cells can activate cellular signaling pathways of mechanical transduction to affect the behavior of nerve cells. Recently, biological scaffolds with various physical properties have been developed as extracellular matrix to regulate the behavior conversion of nerve cell, such as neuronal neurite growth and directional differentiation of neural stem cells, providing a robust driving force for nerve regeneration. This review mainly focused on the biological basis of nerve cells in mechanical transduction. In addition, we also highlighted the effect of the physical cues, including stiffness, mechanical tension, two-dimensional terrain, and electrical conductivity, on neurite outgrowth and differentiation of neural stem cells and predicted their potential application in clinical nerve tissue engineering.

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“…Arising from the Weiss's early work establishing the commonly used concept of “contact guidance” (Harrison, 1910; Weiss, 1934), in which the extension of the oriented nerve fiber was found to use the substrate ultrastructures as a guiding track, substratum topography was suggested to influence the growth and orientation of axons and the morphology of growth cones. Indeed, the in vitro studies, with different substrate topographies derived from in vivo structures (e.g., radial glial fibers (Hatten & Liem, 1981; Rakic, 1971), neurite bundles (Kim, Park, et al, 2018; Nagata & Nakatsuji, 1991), and oriented surrounding extracellular fibers (Antman‐Passig & Shefi, 2016)), showed the directional effects of physical cues on neurite outgrowth and neuronal migration. In the neuronal migration on radial glial fibers, the leading process of neurons was extended along the direction of migration (Edmondson & Hatten, 1987; Gasser & Hatten, 1990), implying that the physical contacts were closely related to the direction of neurite outgrowth and neuronal migration, and the topographical cue would be one of the potent cues guiding the neurons and their processes.…”

Section: Directional Movement Of Neurons On Physical Cuesmentioning

confidence: 99%

Neuro‐taxis: Neuronal movement in gradients of chemical and physical environments

Seo

Youn

Choi

et al. 2020

Developmental Neurobiology

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Environmental chemical and physical cues dynamically interact with migrating neurons and sprouting axons, and in particular, the gradients of environmental cues are regarded as one of the factors intimately involved in the neuronal movement. Since a growth cone was first described by Cajal, more than one century ago, chemical gradients have been suggested as one of the mechanisms by which the neurons determine proper paths and destinations. However, the gradients of physical cues, such as stiffness and topography, which also interact constantly with the neurons and their axons as a component of the extracellular environments, have rarely been noted regarding the guidance of neurons, despite their gradually increasingly reported influences in the case of nonneuronal‐cell migration. In this review, we discuss chemical (i.e., chemo‐ and hapto‐) and physical (i.e., duro‐) taxis phenomena on the movement of neurons including axonal elongation. In addition, we suggest topotaxis, the most recently proposed physical‐taxis phenomenon, as another potential mechanism in the neuronal movement, based on the reports of neuronal recognition of and responses to nanotopography.

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Pioneering Effects and Enhanced Neurite Complexity of Primary Hippocampal Neurons on Hierarchical Neurotemplated Scaffolds

Cited by 5 publications

References 42 publications

Recent progresses in novel in vitro models of primary neurons: A biomaterial perspective

Recent progresses in novel in vitro models of primary neurons: A biomaterial perspective

Physical Cues of Matrices Reeducate Nerve Cells

Neuro‐taxis: Neuronal movement in gradients of chemical and physical environments

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