Pinostrobin alleviates testicular and spermatological damage induced by polystyrene microplastics in adult albino rats

Ijaz, Muhammad Umar; Najam, Saira; Hamza, Ali; Azmat, Rabia; Ashraf, Asma; Unuofin, Jeremiah Oshiomame; Lebelo, Sogolo Lucky; Simal-Gandara, Jesus

doi:10.1016/j.biopha.2023.114686

Cited by 23 publications

(5 citation statements)

References 68 publications

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“…Moreover, FSH and testosterone impacts the release and growth of spermatids. Thus, an appropriate concentration of FSH, LH and Testosterone is desired for spermatogenesis [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

The beneficial effect of Allium Cepa bulb extract on reproduction of rats; A two-generation study on fecundity and sex hormones

Suri,

Khan,

Naeem

et al. 2024

PLoS ONE

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Allium Cepa Linn. (Onions) has extensively been used in traditional medicine, is one of the important Allium species regularly used in our daily diet, and has been the source of robust phenolic compounds. The current study is intended to evaluate the fecundity-enhancing effect of A. Cepa on the reproductive performance of two successive generations of rats; F0 and F1. A. Cepa extract was initially tested for in vitro antioxidant assay via DPPH and ROS, followed by in vivo toxicity testing. In the fecundity assessment, eighteen pairs of male and female rats (n = 36, 1:1, F0 generation) were divided into three groups and dosed with 75mg/kg and 150 mg/kg daily of A. Cepa extract and saline respectively, up to pre-cohabitation, cohabitation, gestation and lactation period. The reproductive performance, including body weight, live birth index, fertility index, and litter size, was assessed. Various parameters like Hematological, Hormonal (FSH, LH, Testosterone, estradiol), antioxidant markers (SOD, Glutathione peroxidase) and lipid profile of F0 and F1 generations were assessed with evaluation of histopathology of male and female organs. Ethanolic extract of A. Cepa showed the greatest antioxidant potential in DPPH and ROS methods. The continued exposure of the F0 and F1 generations to A. Cepa extract did not affect body weight, fertility index, litter size, and survival index. However, semen pH, sperm motility, sperm count, sperm viability, and semen volume were significantly improved in both generations. We have found pronounced fecundity outcomes in both genders of F0 and F1 generations with A. Cepa 150mg/kg/day extract as compared to control. Results showed that A. Cepa significantly increased (P < 0.05) hemoglobin, follicular stimulating hormone (FSH), luteinizing hormone (LH), plasma testosterone and glutathione peroxidase activities, while total lipid, LDL, and cholesterol were significantly decreased (P < 0.05) in both generations. Histology of both generations of animals reveals enhanced spermatogenesis and enhanced folliculogenesis with improved architecture. Altogether, the present results suggest that A. Cepa extract improved fecundity in both male and female rats by improving hormonal activities and oxidative stress.

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“…Moreover, FSH and testosterone impacts the release and growth of spermatids. Thus, an appropriate concentration of FSH, LH and Testosterone is desired for spermatogenesis [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

The beneficial effect of Allium Cepa bulb extract on reproduction of rats; A two-generation study on fecundity and sex hormones

Suri,

Khan,

Naeem

et al. 2024

PLoS ONE

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Section: Multiple Organ Protectionmentioning

confidence: 99%

“…It was reported that PS-MPs increased oxidative stress and the levels of inflammatory cytokines such as IL-6, TNF-α, COX-2, and IL-1β by activating the p38 MAPK pathway in mouse testicular tissues. 61,62 Pinostrobin is a well-known anti-inflammatory and antioxidant natural product. Pinostrobin increases the activities of glutathione reductase (GSR), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) while decreases the levels of MDA and ROS in the PS-MPs-treated rat model.…”

Section: Kidneymentioning

confidence: 99%

“…Finally, pinostrobin alleviates PS-MPs -induced histomorphometric changes in testicular tissues (seminiferous epithelial tubule, tunica propria height, tunica propria width, luminal diameter, and interstitial space), morphological abnormalities in sperm (tail, mid sperm/neck, and head), and reduction in epididymal sperm count and sperm motility. 62 Interestingly, a recent study by using a methotrexate (MTX)-induced ovarian toxicity rat model demonstrated that pinostrobin significantly recovered ovarian histoarchitecture. Pinostrobin significantly decreases serum levels of LH and FSH, as well as ovarian levels of NO, MDA, caspase-3, NF-κB, IL-1β, IL-6, TNF-α, and iNOS.…”

Section: Kidneymentioning

confidence: 99%

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Research Progress on the Pharmacological Effects and Molecular Mechanisms of Pinostrobin

Wang,

Zhao,

Chen

et al. 2023

Natural Product Communications

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Pinostrobin (5-hydroxy-7-methoxyflavanone) is an active flavonoid substance. It is widely present in many traditional Chinese herbs, including Carya cathayensis leaves, Boesenbergia rotunda and propolis. Pinostrobin possesses many pharmacological activities without significant toxicity. In addition to anti-inflammatory and antioxidant effects, pinostrobin also exerts antitumor effects. Pinostrobin is able to protect against infection by several viruses, bacteria, fungi, and parasites. Pinostrobin reduces the expression of proprotein convertase subtilisin/kexin type 9 (PCSK9), which is a promising target of lipid-lowering drugs. It also reduces high uric acid levels while not affecting the normal uric acid levels. Furthermore, it has protective effects on the liver, kidney, bone, skin, reproductive system, stomach and nerves. Due to its multifunctional effects and safety, pinostrobin has advantages for development as a novel drug for clinical application. Based on this, this current article summarized the research progress on the pharmacological effects and mechanisms of pinostrobin, to provide a reference for further development.

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“…Pinostrobin (5-hydroxy-7-methoxy flavanone; PN) is a member of flavonoids and an ether ( Figure 1 ) and a dietary bioflavonoid isolated from natural resources including plants, Pinus strobus , 4 Cajanus cajan (L.), 25 rhizomes of Boesenbergia rotunda 26 and finger root (Boesenbergia rotunda) . 27 Numerous biological potentials exhibited by this flavanone including antioxidants, 28 anti-inflammatory, 29 augmentations of inflammatory cytokines (interleukin-1β, tumour necrosis factor (TNF)-α) 30 and anti-ulcerogenic actions. 26 Moreover, in vitro , studies revealed outstanding inhibitory efficacy of PN against the growth of breast cancer, 31 cervical, 32 lung cancers, 33 HL-60 and K562 leukaemia cells.…”

Section: Introductionmentioning

confidence: 99%

Pinostrobin attenuates azoxymethane-induced colorectal cytotoxicity in rats through augmentation of apoptotic Bax/Bcl-2 proteins and antioxidants

Ali Abed Wahab,

Ain Salehen,

Abdulla

et al. 2023

SAGE Open Medicine

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Objectives: Pinostrobin (5-hydroxy-7-methoxyflavanone; PN) is a natural active ingredient with numerous biological activities extensively utilized in tumour chemotherapy. The present study investigates the chemo-preventive potentials of PN on azoxymethane-mediated colonic aberrant crypt foci in rats. Methods: Sprague Dawley rats clustered into five groups, normal control (A) and cancer controls were subcutaneously injected with normal saline and 15 mg/kg azoxymethane, respectively, and nourished on 10% tween 20 and fed on 10% tween 20; reference control (C), injected with 15 mg/kg azoxymethane and injected (intraperitoneal) with 35 mg/kg 5-fluorouracil (5-FU); D and E rat groups received a subcutaneous injection of 15 mg/kg azoxymethane and nourished on 30 and 60 mg/kg of PN, respectively. Results: The acute toxicity trial showed a lack of any abnormal signs or mortality in rats ingested with 250 and 500 mg/kg of PN. The gross morphology of colon tissues revealed significantly lower total colonic aberrant crypt foci incidence in PN-treated rats than that of cancer controls. Histological examination of colon tissues showed increased aberrant crypt foci availability with bizarrely elongated nuclei, stratified cells and higher depletion of the submucosal glands in cancer controls. PN treatment caused positive modulation of apoptotic (Bax and Bcl-2) proteins and inflammatory cytokines (TNF-α, IL-6 and IL-10). Moreover, rats fed on PN had significantly higher antioxidants (superoxide dismutase) and lower malondialdehyde concentrations in their colon tissue homogenates. Conclusion: The chemoprotective efficiency of PN against azoxymethane-induced aberrant crypt foci is shown by lower aberrant crypt foci values and higher aberrant crypt foci inhibition percentage, possibly through augmentation of genes responsible for apoptotic cascade and inflammations originating from azoxymethane oxidative stress insults.

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Pinostrobin alleviates testicular and spermatological damage induced by polystyrene microplastics in adult albino rats

Cited by 23 publications

References 68 publications

The beneficial effect of Allium Cepa bulb extract on reproduction of rats; A two-generation study on fecundity and sex hormones

The beneficial effect of Allium Cepa bulb extract on reproduction of rats; A two-generation study on fecundity and sex hormones

Research Progress on the Pharmacological Effects and Molecular Mechanisms of Pinostrobin

Pinostrobin attenuates azoxymethane-induced colorectal cytotoxicity in rats through augmentation of apoptotic Bax/Bcl-2 proteins and antioxidants

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