Pinocytosis and Membrane Dilation in Uranyl-Treated Plant Roots

Wheeler, Harry; Hanchey, Penelope

doi:10.1126/science.171.3966.68

Cited by 46 publications

(35 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Uranyl ions then freely diffuse into the cell interior to crystallize with some intracellular substance. These results support the suggestion that uranyl does not diffuse into nondamaged cells (17). Greater numbers of cells showing severe damage were found in leaves treated with toxin-uranyl than in leaves treated with toxin-water.…”

Section: Uranium Localization In Diseased Oats 14)supporting

confidence: 81%

“…Wheeler and Hanchey (17) found that uranyl-induced dilation of the plasmalemma made possible the identification of vesicles derived from this membrane in roots. However vesicle origin could not be determined by comparing membrane widths in leaves as the width of both the tonoplast and the plasmalemma varied along their lengths in different areas of the cell.…”

Section: Uranium Localization In Diseased Oats 14)mentioning

confidence: 99%

See 1 more Smart Citation

Localization of Crystals in Diseased Oats Treated with Uranyl Acetate

Easton

Hanchey

1972

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Uranyl acetate, a suppressor of victorin-induced electrolyte leakage in oat leaves when applied together with, or before, victorin, also suppressed victorin-induced changes in ultrastructure. Uranyl crystallized in cell walls and near the plasmalemma of vascular cells, but was excluded from the protoplasm. Fewer crystals occurred near the plasmalemma when leaves were allowed to take up uranyl and victorin simultaneously than when uranyl alone was absorbed, but deposition in cell wails was similar in the two treatments. No differences in crystal distribution were found in uranyl-treated leaves which subsequently took up either water or victorin. The most striking effect of prolonged exposure to uranyl was increased vesicular activity in the protoplasm, formation of complex concentric membranes, and tonoplast damage. Following victorin treatment, uranyl post-treatment was ineffective in suppressing electrolyte leakage or preserving normal cellular ultrastructure. More severe ultrastructural damage was found in victorintreated leaves after uranyl post-treatments than after posttreatment with water, a result of victorin-induced damage which facilitates uranyl entry into the protoplasm.Alterations in membrane permeability are characteristic of many diseased plant tissues, and these changes may result in secondary metabolic effects on respiration, photosynthesis, hormone activities, and phenolic levels (3,20). Permeability changes in oats (Avena sativa L.) infected with Helminthosporium victoriae are caused by a pathotoxic substance, victorin, secreted by the fungus. The disease is an excellent system for studies on pathological changes in physiology and ultrastructure of plants (13,16,18). The pathotoxin causes loss of electrolytes and labeled compounds from tissues within minutes after treatment of susceptible oat varieties (11,15). The earliest detected ultrastructural change in diseased oats is near the plasmalemma, suggesting that the primary effect of victorin is on plasmalemma permeability (7).Uranyl salts suppressed both blight symptoms and electrolyte leakage in victorin-treated leaves of susceptible oat varieties, but did not prevent victorin-induced inhibition of root growth (4). These compounds are effective whether supplied

show abstract

Section: Uranium Localization In Diseased Oats 14)supporting

confidence: 81%

Section: Uranium Localization In Diseased Oats 14)mentioning

confidence: 99%

Localization of Crystals in Diseased Oats Treated with Uranyl Acetate

Easton

Hanchey

1972

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Uraniumephosphate complex was found also in meristematic walls and vacuoles of barley and oak. (Wheeler and Hanchey, 1971;Robards and Robb, 1972), but the mechanism of uptake is unknown. Phytochelatins, as common chelators of heavy metals in plants, are not induced in the presence of uranyl ions (Grill et al, 1987).…”

Section: Introductionmentioning

confidence: 98%

Uranium uptake by hydroponically cultivated crop plants

Soudek

Benešová

Dvořáková

et al. 2011

Journal of Environmental Radioactivity

View full text Add to dashboard Cite

“…Furthermore, theoretical arguments claiming that plants cannot generate sufficient pressure-volume work to form vesicles against the strong turgor pressure within the cell have been offered (Cram, 1980), and these have undoubtedly discouraged exploratory experiments into the occurrence of endocytotic processes in plants. Thus, despite observations that higher plants contain coated vesicles (Mersey et al, 1985), can internalize heavy metals (Wheeler and Hanchey, 1971;Hubner, Depta, and Robinson, 1985), and can even take up larger molecules once their cell walls have been removed (and turgor pressure drops to zero), (Joachim and Robinson, 1984;Tanchak et al, 1984;Hillmer, Depta, and Robinson, 1986), the evidence for receptor-mediated endocytosis has been insufficient to motivate a thorough evaluation of the process.…”

Section: Introductionmentioning

confidence: 99%

Receptor-Mediated Endocytosis in Plant Cells

Horn¹,

Heinstein²

1989

The Plant Cell

View full text Add to dashboard Cite

We have employed fluorescein and 1251-labeled elicitors of the defense response in soybeans to monitor the cellular distribution and movement of elicitors following their addition to a soybean cell suspension culture. Our results indicate that the macromolecular elicitors first bind to the cell surface and then internalize in a temperature-and energy-dependent endocytotic process. Within a few hours, virtually all of the elicitor is concentrated in the major vacuole or tonoplast of the cell. Nonspecific (control) proteins neither bound to the cell surface nor internalized in parallel assays.

show abstract

Pinocytosis and Membrane Dilation in Uranyl-Treated Plant Roots

Cited by 46 publications

References 11 publications

Localization of Crystals in Diseased Oats Treated with Uranyl Acetate

Localization of Crystals in Diseased Oats Treated with Uranyl Acetate

Uranium uptake by hydroponically cultivated crop plants

Receptor-Mediated Endocytosis in Plant Cells

Contact Info

Product

Resources

About