Pinocembrin inhibits the proliferation and migration and promotes the apoptosis of ovarian cancer cells through down-regulating the mRNA levels of N-cadherin and GABAB receptor

Gao, Jun; Lin, Shixin; Gao, Yao; Zou, Xia; Jun, Zhu; Chen, Man; Wan, Hong; Zhu, Hong

doi:10.1016/j.biopha.2019.109505

Cited by 31 publications

(23 citation statements)

References 30 publications

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“…Sub-G0/ G1 cells are the hallmark of apoptosis, and the flow cytometry results also confirmed the apoptosis inducing effect of HMF. Interestingly, HMF induced necrosis at relative higher concentration, which was consistent with the chemotherapeutic agent cisplatin that also stimulates necrosis in cancer cells [23]. It has been reported that inducing necrosis of tumor tissues could suppress tumor growth in mouse xenograft bladder cancer models [24], so we speculated necrosis induction of HMF might also be responsible for antiproliferative activity of HMF.…”

Section: Discussionsupporting

confidence: 79%

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Xu³

et al. 2020

BMC Complement Med Ther

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Background: Lung cancer has the highest morbidity and mortality in the world and novel treatment strategies are still needed. Haimufang decoction (HMF) is a patented clinical prescription of traditional Chinese medicine for lung cancer treatment. HMF is composed of four herbs and has been applied clinically in advanced cancer patients. However, its therapeutic mechanisms are still unclear. This study aims to elucidate the possible mechanisms of HMF for the treatment of lung cancer. Methods: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was applied for evaluating the proliferative effect of HMF in lung cancer cells and monocyte macrophage RAW264.7 cells. Flow cytometer was used to detect the effects of HMF on cell cycle and apoptosis, and western blotting was employed to explore the potential apoptotic mechanisms of HMF on lung cancer cells. For immunomodulatory effect, co-culture system was used to detect the activation of macrophage RAW264.7 cells when treated with HMF, and neutral red assay was used to measure the effect of HMF on the phagocytosis of the activated macrophages. Enzyme linked immunosorbent assay, flow cytometer, and immunofluorescence staining method were employed for the investigation on the underlying mechanisms of the immunomodulatory effect on RAW264.7 induced by HMF.

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Section: Discussionsupporting

confidence: 79%

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Xu³

et al. 2020

BMC Complement Med Ther

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“…Pinocembrin (PCB; 5,7-dihydroxyflavanone) is a pharmacologically active ingredient of Pinus heartwood, Populus, Sparattosperma leucanthum, Eucalyptus, and Euphorbia with diverse pharmacological effects (6) that has been extensively applied for the treatment of microbial infection (7), inflammation (8), ischemia-reperfusion injury (9), and atherosclerosis (10). In addition, numerous recent studies have reported that PCB has anticancer activities by targeting the cell cycle, apoptosis, and the metastatic potential of various solid tumors (11)(12)(13)(14). However, the effect of PCB for the treatment of BC remains unclear.…”

Section: Highlights Introductionmentioning

confidence: 99%

Pinocembrin Inhibits the Proliferation and Metastasis of Breast Cancer via Suppression of the PI3K/AKT Signaling Pathway

Zhu¹,

Li²,

Wang³

et al. 2021

Front. Oncol.

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The survival rate of breast cancer (BC) patients remains poor, thus the identification of safe and effective new drugs is crucial to improve therapeutic outcomes and overall survival. Pinocembrin (PCB), a pharmacologically active ingredient of Pinus heartwood, Eucalyptus, Euphorbia, Populus, and Sparattosperma leucanthum, has been widely applied for the treatment of various diseases and possesses anticancer activities. In vitro assays were performed to investigate the antiproliferation and antimetastasis activities of PCB in BC cells. A tumorigenesis assay with the use of murine BC models was performed to assess the antiproliferation activities of PCB in vivo. Moreover, the molecular mechanisms underlying the anticancer activities of PCB in BC cells were explored. The results showed that the anti-inhibitory and antiproliferation activities of PCB in BC might involve cell cycle (G2/M phase) arrest and apoptosis. PCB downregulated the expression levels of proteins involved in cell cycle progression and apoptosis, including cyclinB1, Cdc2, PARP1, Bcl-2, and survivin, and upregulated protein levels of cleaved PARP1, cleaved caspase3, cleaved caspase9, and BAX. In a murine subcutaneous tumor model, PCB suppressed the growth of MCF-7 cells in vivo. Low concentrations of PCB also significantly inhibited the migration and invasion abilities of BC cells. Mechanistically, PCB administration was correlated to suppression of the PI3K/AKT signaling pathway. Inhibition of the proliferation of BC cells by PCB involved cell cycle (G2/M phase) arrest and apoptosis in vitro and in vivo. Low concentrations of PCB also significantly inhibited the migration and invasion abilities of BC cells. These findings suggest that PCB might be an effective agent for treatment of BC patients.

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“…Gao et al, investigate the effect of different concentrations of pinocembrin (100-200 µM) in vitro on SKOV3 human ovarian cancer cell lines using some consecrated assays (cell scratch assay, Annexin V-FITC/PI staining, Western blot and RT-PCR). Results revealed that this molecule inhibited the proliferation, migration and promoted apoptosis of the tested ovarian cancer cell line [78]. Kumar et al, have demonstrated that pinocembrin (50, 100 and 200 µM) induced apoptosis in HCT116 human colon cancer cell lines.…”

Section: Antitumor Propertiesmentioning

confidence: 98%

Recent Advances Regarding the Phytochemical and Therapeutic Uses of Populus nigra L. Buds

Kis

Avram

Pavel

et al. 2020

Plants

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Populus nigra L. (Salicaceae family) is one of the most popular trees that can be found in deciduous forests. Some particularities that characterize the Populus genus refer to the fact that it includes more than 40 species, being widespread especially in Europe and Asia. Many residues, parts of this tree can be used as a bioresource for different extracts as active ingredients in pharmaceuticals next to multiple benefits in many areas of medicine. The present review discusses the latest findings regarding the phytochemical composition and the therapeutic properties of Populus nigra L. buds. The vegetal product has been described mainly to contain phenolic compounds (phenols, phenolic acids and phenylpropanoids), terpenoids (mono and sesquiterpenoids), flavones (e.g., apigenol and crysin), flavanones (e.g., pinocembrin and pinostrombin), caffeic/ferulic acids and their derivates, and more than 48 phytocompounds in the essential oils. The resinous exudates present on the buds have been the major plant source used by bees to form propolis. Several studies depicted its antioxidant, anti-inflammatory, antibacterial, antifungal, antidiabetic, antitumor, hepatoprotective, hypouricemic properties and its effects on melanin production. All these lead to the conclusion that black poplar buds are a valuable and important source of bioactive compounds responsible for a wide range of therapeutic uses, being a promising candidate as a complementary and/or alternative source for a large number of health problems. The aim of the review is to gather the existing information and to bring an up to date regarding the phytochemical and therapeutic uses of Populus nigra L. buds.

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Pinocembrin inhibits the proliferation and migration and promotes the apoptosis of ovarian cancer cells through down-regulating the mRNA levels of N-cadherin and GABAB receptor

Cited by 31 publications

References 30 publications

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Pinocembrin Inhibits the Proliferation and Metastasis of Breast Cancer via Suppression of the PI3K/AKT Signaling Pathway

Recent Advances Regarding the Phytochemical and Therapeutic Uses of Populus nigra L. Buds

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