Pinitol suppresses tumor necrosis factor-α-induced invasion of prostate cancer LNCaP cells by inhibiting nuclear factor-κB-Mediated matrix Metalloproteinase-9 expression

Jayasooriya, Rgpt; Kang, C-H; Park, SR; Choi, Yoon-Hee; Gy, Kim

doi:10.4314/tjpr.v14i8.6

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…This compound is soluble in water and slightly soluble in ethanol, and it is capable of activating the non‐canonical insulin signalling pathway. This natural inositol has been proved to be a potential pharmacological tool for its positive effects in many different diseases: anticancer (Jayasooriya et al, 2015) antidiabetic (Bates et al, 2000; Gao et al, 2015), antioxidant (Sivakumar et al, 2010), hepatoprotective (Choi et al, 2009), immune‐suppressor (Kim et al, 2005; Lee et al, 2007), anti‐osteoporosis (Liu et al, 2012), antiageing (Hada et al, 2013) and brain insulin‐resistance associated disorders, such as Alzheimer's disease (AD) (Lee et al, 2014; López‐Gambero et al, 2020; Pitt et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

d‐Pinitol promotes tau dephosphorylation through a cyclin‐dependent kinase 5 regulation mechanism: A new potential approach for tauopathies?

Medina-Vera

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Rivera

et al. 2022

British J Pharmacology

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Background and Purpose: Recent evidence links brain insulin resistance with neurodegenerative diseases, where hyperphosphorylated tau protein contributes to neuronal cell death. In the present study, we aimed to evaluate if D-pinitol inositol, which acts as an insulin sensitizer, affects the phosphorylation status of tau protein.Experimental Approach: We studied the pharmacological effect of D-pinitol on insulin signalling and tau phosphorylation in the hippocampus of Wistar and Zucker rats.To this end, we evaluated by western blotting the Akt pathway and its downstream proteins as being one of the main insulin-mediator pathways. Also, we explored the functional status of additional kinases phosphorylating tau, including PKA, ERK1/2, AMPK and CDK5. We utilized the 3xTg mouse model as a control for tauopathy, since it carries tau mutations that promote phosphorylation and aggregation.

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Section: Introductionmentioning

confidence: 99%

d‐Pinitol promotes tau dephosphorylation through a cyclin‐dependent kinase 5 regulation mechanism: A new potential approach for tauopathies?

Medina-Vera

Navarro

Rivera

et al. 2022

British J Pharmacology

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“…Interestingly, pinitol has also been shown to inhibit the activation of the nuclear factor-kB pathway, a key regulator of inflammation, and the expression of matrix metalloproteinase-9, which has been implicated in atherosclerotic plaque rupture. 16 Oxidative stress is the result of an imbalance in the ratio of oxidants to antioxidants. Excessive production of ROS triggered by ox-LDL has long been documented as a key effector in atherogenesis.…”

Section: Discussionmentioning

confidence: 99%

Pinitol Protects Against Ox-Low-Density Lipoprotein-Induced Endothelial Inflammation and Monocytes Attachment

Chen

et al. 2022

Journal of Cardiovascular Pharmacology

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Atherosclerosis is a cardiovascular disease that affects a majority of people around the world at old age. Atherosclerosis is slow to develop and challenging to treat. Endothelial dysfunction caused by oxidative stress, inflammation, and other pathological factors drives the process of atherogenesis. LOX-1 is one of the main scavenging receptors for oxidized low-density lipoprotein (ox-LDL) and contributes to atherogenesis by inducing overproduction of reactive oxygen species, increased expression of proinflammatory cytokines, and secretion of cellular adhesion molecules. In addition, activation of LOX-1 inhibits the expression of KLF2, a key protective factor against atherosclerosis. In this study, we investigated the effects of pinitol, and naturally occurring cyclic polyol, on endothelial dysfunction induced by ox-LDL. Our findings show that pinitol revealed a good safety profile, as evidenced by reducing lactate dehydrogenase release in human aortic endothelial cells. In our experiments, pinitol reduced the production of reactive oxygen species and expression of IL-6 and monocyte chemoattractant protein-1 induced by ox-LDL. Pinitol also significantly reduced the attachment of THP-1 monocytes to endothelial cells via downregulation of vascular cellular adhesion molecule-1 and E-selectin. Importantly, we found that pinitol reduced the expression of LOX-1 induced by ox-LDL and rescued the expression of KLF2, which is dependent on ERK5 expression. Together, our findings provide notable evidence that pinitol may have potential implication in the prevention and treatment of atherosclerosis.

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“…β-Sitosteryl-3-O-β-D-glucoside is known as Daucosterol, has hepatoprotective, hypoglycemic anticancer, antimicrobial, antioxidant activities 36,42 . It also has anti-allergic and anti-inflammatory properties 43 . 3-O-methyl-D-Chiro-Inositol is also called D-Pinitol, has substantial pharmacological importance; it has extensively used in the treatment of cancer, Alzheimer's disease, Type-2 Diabetes, and hepatoprotective activity 44,47 .…”

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confidence: 99%

Untitled

2022

IJPSR

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The present research effort was undertaken to isolate phytoconstituents from pharmacologically active extracts of stem bark of Albizia stipulata based on in-vitro pharmacological screening and their subsequent characterization. Methods: Crude extracts of bark of Albizia stipulata were prepared using various solvents such as methanol, hydro alcohol, and water. These extracts were screened for in-vitro pharmacological activities like antioxidant, anti-inflammatory, and anti-diabetic activities. The active extract was subjected to column chromatography through a mixture of solvents to get fractions and eluted fractions were run in TLC using various mobile phases with different solvent ratios. The isolated compounds were subjected to IR, 1H NMR, 13CNMR and LC-MS spectral analysis for chemical characterization. Results: The methanol extract of bark of Albizia stipulata was potent compared to other extracts. This extract was subjected to column chromatography to get fractions, and eluted fractions were run in TLC. The fractions with similar R f values to standard were united and crystallized. The spectral analysis confirmed that the isolated compounds were found to be β-Sitosteryl-3-O-β-D-glucoside and 3 -O methyl D-Chiro Inositol. Conclusion: Various extracts from the bark of the plant Albizia stipulata were prepared. Β-sitosteryl-3-O-β-D-glucoside and 3-O methyl D-Chiro Inositol were isolated from the methanol extract of bark and characterized. INTRODUCTION: Herbs used in Ayurveda offer biologically active molecules and lead structures for modification.

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Pinitol suppresses tumor necrosis factor-α-induced invasion of prostate cancer LNCaP cells by inhibiting nuclear factor-κB-Mediated matrix Metalloproteinase-9 expression

Abstract: Purpose: To investigate the mechanism by which pinitol inhibits tumor necrosis factor-α (TNF-α)-induced expression of matrix

Cited by 6 publications

References 21 publications

d‐Pinitol promotes tau dephosphorylation through a cyclin‐dependent kinase 5 regulation mechanism: A new potential approach for tauopathies?

d‐Pinitol promotes tau dephosphorylation through a cyclin‐dependent kinase 5 regulation mechanism: A new potential approach for tauopathies?

Pinitol Protects Against Ox-Low-Density Lipoprotein-Induced Endothelial Inflammation and Monocytes Attachment

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