Pinicolol B from Antrodia cinnamomea induces apoptosis of nasopharyngeal carcinoma cells

Wu, Tsung-Ru; Huang, Tzu Ting; Martel, Jan; Liau, Jian-Ching; Chiu, Chen-Yaw; Leu, Yann‐Lii; Jian, Wei-Ting; Chang, I-Te; Lu, Cuihua; Ojcius, David M.; Ko, Yun‐Fei; Lai, Hsin‐Chih; Young, John Ding‐E

doi:10.1016/j.jep.2017.02.008

Cited by 6 publications

(2 citation statements)

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“…The anti-cancer and anti-metastasis activities of ACEE may be produced by triterpenoids and the lanostanoid compound pinicolol B which have been identified in the extract studied here (triterpenoids, 34.3%, w/w; pinicolol B, 91 ppm; see Methods; more information about the chemical analysis of ACEE is also found in our previous study) 25 . These triterpenoid and lanostenoid compounds have been shown to produce anticancer effects in previous studies 26–28 .…”

Section: Discussionmentioning

confidence: 91%

Antrodia cinnamomea induces anti-tumor activity by inhibiting the STAT3 signaling pathway in lung cancer cells

Huang

Lan

Chen

et al. 2019

Sci Rep

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We examined the effects of an Antrodia cinnamomea ethanol extract (ACEE) on lung cancer cells in vitro and tumor growth in vivo. ACEE produced dose-dependent cytotoxic effects and induced apoptosis in Lewis lung carcinoma (LLC) cells. ACEE treatment increased expression of p53 and Bax, as well as cleavage of caspase-3 and PARP, while reducing expression of survivin and Bcl-2. ACEE also reduced the levels of JAK2 and phosphorylated STAT3 in LLC cells. In a murine allograft tumor model, oral administration of ACEE significantly inhibited LLC tumor growth and metastasis without affecting serum biological parameters or body weight. ACEE increased cleavage of caspase-3 in murine tumors, while decreasing STAT3 phosphorylation. In addition, ACEE reduced the growth of human tumor xenografts in nude mice. Our findings therefore indicate that ACEE inhibits lung tumor growth and metastasis by inducing apoptosis and by inhibiting the STAT3 signaling pathway in cancer cells.

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Section: Discussionmentioning

confidence: 91%

Antrodia cinnamomea induces anti-tumor activity by inhibiting the STAT3 signaling pathway in lung cancer cells

Huang

Lan

Chen

et al. 2019

Sci Rep

Self Cite

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“…Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach (cell colonies and cancer stem cells). Wu, Huang, et al (2017) used the MTS assay to evaluate the effects of Pinicolol B, a natural product, on nasopharyngeal carcinoma cell (TW02 and TW04) proliferation. Antiproliferative effects of Ontario grown onions have been studied by Murayyan, Manohar, Hayward, and Neethirajan (2017) in colorectal adenocarcinoma cells (Caco-2) using the MTS assay.…”

Section: Introductionmentioning

confidence: 99%

In vitro assays and techniques utilized in anticancer drug discovery

Ediriweera

Tennekoon

Samarakoon

2018

J of Applied Toxicology

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Development of a cancer is a multistep process and six major hallmarks of cancer that are known to control malignant transformation have been described. Anticancer drug development is a tedious process, requiring a number of in vitro, in vivo and clinical studies. In vitro assays provide an initial platform for cancer drug discovery approaches. A wide range of in vitro assays/techniques have been developed to evaluate each hallmark feature of cancer and selection of a particular in vitro assay or technique mainly depends on the specific research question (s) to be examined. In the present review, we have described some commonly utilized in vitro assays and techniques used to examine cell viability/proliferation, apoptosis, cellular senescence, invasion and migration, oxidative stress and antioxidant effects, gene and protein expression, angiogenesis and genomic alterations in cancer drug discovery. Additionally, uses of modern techniques such as high throughput screening, high content screening and reporter gene assays in cancer drug discovery have also been described.

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