Pinelloside, an Antimicrobial Cerebroside from Pinellia ternata.

Antibacterial and xanthine oxidase inhibitory cerebrosides from Fusarium sp. IFB‐121, and endophytic fungus in Quercus variabilis

“…The cerebroside fraction extracted with 1:1 CHCl 3 /MeOH from the solid-state culture of the endophyte was first separated by a combination of Si-gel column chromatography and gel filtration on a Sephadex-LH 20 column, and the final purification was achieved by semipreparative HPLC to afford two antibacterial and XO inhibitory white amorphous solids (1 and 2). Subsequent TLC comparisons demonstrated that the polarity of both isolates was fairly close to, but different from, that of pinelloside, a new monohexosylceramide characterized recently from the tubers of Perilla frutescens (8). Furthermore, the ESI-MS/MS mass spectra of both compounds showed intense fragment peaks afforded through the elimination of a single hexose moiety from the protonated molecular ions.…”

“…A solution of 1 or 2 (15 mg) in MeOH (2 mL), water (0.2 mL), and 12 N HCl (0.2 mL) was refluxed for 7 h as described elsewhere (8,9). After cooling, the reaction mixture was dried with a stream of N 2 to yield a residue that was dissolved in 9:1 MeOH/H 2 O (8 mL) and subsequently extracted three times with n-hexane (8 mL each).…”

New Sphingolipids with a Previously Unreported 9‐Methyl‐C20‐sphingosine Moiety from a Marine Algous Endophytic Fungus Aspergillus niger EN‐13

“…The absolute stereochemistry for sphingolipids is commonly characterized by the comparison of the NMR data with related literature reports [1,7,11,12]. For 1, the configurations at C-2, C-2¢, and C-3 were determined based on the proton chemical shifts and coupling constants at d [13].…”

“…They play an important role in recognition of pathogens, signal transduction, cell differentiation, and apoptosis of cancer cells. Many sphingolipids have been found to exhibit antihepatotoxic, antitumor, antifungal, antiviral, neuritogenic, and immunoregulatory activities [1][2][3][4] and have drawn more and more interest from chemical and medicinal researchers. Since the 1990s, marine derived fungi have been recognized as a rich source of novel bioactive metabolites because their environments differ from those of terrestrial fungi [5].…”

Extraction optimization and characterization of polysaccharide antioxidants from <i>Pinellia ternata</i> (Thunb) Breit rhizome

“…is an important medicinal plant from the Araceae family, and is widely distributed in Anhui, Sichuan and Hubei provinces of China. Its Rhizoma (PR) has been used in Traditional Chinese Medicines (TCMs) for its antiemetic, antitussive, anti-tumor, antimicrobial, anti-obesity, anticonvulsant activities and other therapeutic efficacies [1][2][3][4][5][6]. Studies have shown that the main constituents of PR are proteins, alkaloids, polysaccharides and organic acids [7][8][9][10].…”