Pinellia pedatisecta lectin exerts a proinflammatory activity correlated with ROS-MAPKs/NF-κB pathways and the NLRP3 inflammasome in RAW264.7 cells accompanied by cell pyroptosis

Wang, Wei; Mao, Shanhu; Yu, Hongli; Wu, Hao; Shan, Xue-Lian; Zhang, Xingde; Cui, Guojing; Liu, Xianqiong

doi:10.1016/j.intimp.2018.11.002

Cited by 36 publications

(15 citation statements)

References 36 publications

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“…ROS not only causes oxidative stress response, but also triggers an inflammatory cascade involved in the MAPK pathway that produces various pro-inflammatory cytokines. Wang et al have put forward that proinflammatory activity was correlated with the ROS-MAPK pathway and was involved in the NLRP3 inflammasome activation (Wang et al, 2019). In our experiment, AA increased the production of ROS dose-dependently compared with un-treated KCs.…”

Section: Discussionsupporting

confidence: 53%

Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells

Nan

Yuan

Yan

et al. 2019

Food and Agricultural Immunology

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Acrylamide (AA) is an important product of the Maillard reaction. Studies have demonstrated that AA caused oxidative stress damage in liver, which in turn triggered an inflammatory response, and the activation of NOD-like receptor protein-3 (NLRP3) inflammasome played a key factor in regulating inflammation. Therefore, we hypothesized NLRP3 inflammasome activation was involved in AAinduced inflammatory response. AA induced the reactive oxygen species (ROS) overproduction, accompanied by the MAPK pathway activation, which resulted in NLRP3 inflammasome formation, and eventually increased IL-1β and IL-18 release. The activation of the MAPK pathway was inhibited when using ROS scavenger (NAC). And when MAPK selective inhibitors were used, KCs viability was increased though AA-treated. Meanwhile the NLRP3 inflammasome activation was inhibited, which decreased the release of the cellular inflammatory secretion factors IL-1β and IL-18. Overall, the activation of ROS-MAPK-NLRP3-IL-1β signalling axis induced by AA plays an important role in the process of inflammation.Signaling pathways involved in inflammation induced by AA. After AA stimulated KCs, a large amount of ROS were released. ROS activated MAPK signaling pathway to promote the activation of NLRP3 inflammasome. Activation of NLRP3 signaling activated Caspase-1, resulting in the maturation of IL-1β and IL-18. The activation of NLRP3 inflammasome was inhibited and the levels of cytoinflammatory factor IL-1β and IL-18 were decreased when using MAPK selective inhibitors.

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Section: Discussionsupporting

confidence: 53%

Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells

Nan

Yuan

Yan

et al. 2019

Food and Agricultural Immunology

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“…However, in recent years, a large number of literatures have reported that ingredients of Chinese medicine can regulate the ASC of RAW264.7 cells and activate NLRP3 pathway. Pinellia pedatisecta lectin was reported to bind with NLRP3 through ASC following by caspase-1 in the inflammation of RAW264.7 cells (Wang et al, 2019 ). And luteolin was reported to decrease production of ROS and expression of NLRP3, ASC, caspase-1, IL-18 and IL-1β proteins in RAW264.7 cells, which were incubated with LPS (Zhang et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Mechanisms of Macrophage Immunomodulatory Activity Induced by a New Polysaccharide Isolated From Polyporus umbellatus (Pers.) Fries

Liu

Lai

et al. 2020

Front. Chem.

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Bladder cancer is one of the most malignant tumors closely associated with macrophage immune dysfunction. The Chinese medicine polyporus has shown excellent efficacy in treating bladder cancer, with minimal side effects. However, its material basis and mechanism of action remain unclear. A new water-soluble polysaccharide (HPP) with strong immunomodulatory activity was isolated from the fungus Polyporus umbellatus (Pers.) Fries. HPP had an average molecular weight of 6.88 kDa and was composed mainly of an <-(1→ 4)-linked D-galactan backbone. The immunomodulatory activity of HPP was determined in vitro, and the results revealed that it could obviously increase the secretion of immune factors by IFN-γ-stimulated macrophages, including nitric oxide (NO), interleukin-6 (IL-6), interleukin-1β (IL-1β), RANTES and interleukin-23 (IL-23), and the expression of the cell membrane molecule CD80. In addition, HPP was recognized by Toll-like receptor 2 (TLR2) and activated the signaling pathways of NF-κB and NLRP3 in a bladder cancer microenvironment model, indicating that HPP could enhance host immune system function. These findings demonstrated that HPP may be a potential immune modulator in the treatment of immunological diseases or bladder cancer therapy.

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“…Pinellia pedatisecta is a widely used herb in Chinese medicine. Its proinflammatory toxicity is related to Pinellia pedatisecta lectin (PPL), which activates the reactive oxygen species (ROS)/mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NF-κB) pathway and NOD-like receptor protein 3 (NLRP3) inflammasome [42].The ingredients in the compounds also include benzodiazepines, which the main effects are sedation, hypnosis, and decreased anxiety [43].This is likely to be the mechanism by which HJZT promotes sleep in mice. The aforementioned Chinese medicine monomers may be the main medicinal components that are useful for the treatment of migraine, but further studies are needed to determine whether they are compatible with each other, whether new ingredients are produced, and the specific effects of the components on the clinical efficacy of the compound.…”

Section: Discussionmentioning

confidence: 99%

Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model

Wang

Zhao²,

et al. 2020

Chin Med

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Background Migraine is painful disease in which neurotransmitters related to pain transmission play an important role. Hejie Zhitong prescription (HJZT) has been used in the clinic as an effective prescription for the treatment of migraine for many years. Our team aimed to further explore its antimigraine mechanism based on previous research results and to explore the inhibitory effect of HJZT on the transmission of pain related to nitroglycerine (NTG)-induced migraine as well as the synergistic effect of HJZT with pentobarbital sodium on promoting sleep. Methods Sixty mice were randomly assigned to groups and received the corresponding interventions. Sleep latency and sleep time were recorded to calculate the incidence of sleep. Forty-eight Wistar rats were randomly assigned and administered an intervention corresponding to their group. Calcitonin gene-related peptide (CGRP), serotonin (5-HT), substance P (SP), and cholecystokinin (CCK) levels were measured using ELISAs. Levels of the cannabinoid receptor type 1 (CB1R) and cyclooxygenase-2 (COX-2) protein were assessed using immunohistochemistry. The expression of the CGRP and CCK mRNAs in the midbrain and trigeminal ganglion (TG) were measured using real-time quantitative PCR. Results HJZT promoted the occurrence of sleep in mice. HJZT downregulated COX-2 expression in the midbrain and TG of rats but upregulated the expression of the CB1R, and decreased the plasma level of the CGRP protein and expression of its mRNA in the midbrain and TG. It also downregulated the expression of the CCK mRNA in the midbrain and TG. The high-dose HJZT treatment increased plasma 5-HT levels, but did not induce changes in the plasma levels of the SP or CCK protein. Conclusions HJZT exerts a synergistic effect with pentobarbital sodium on promoting sleep. As for anti-migraine, HJZT can inhibits the expression of nociceptive transmission-associated neurotransmitters, including 5-HT, CGRP and CCK, which may be related to its upregulation of CB1R and downregulation of COX-2.

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Pinellia pedatisecta lectin exerts a proinflammatory activity correlated with ROS-MAPKs/NF-κB pathways and the NLRP3 inflammasome in RAW264.7 cells accompanied by cell pyroptosis

Cited by 36 publications

References 36 publications

Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells

Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells

Mechanisms of Macrophage Immunomodulatory Activity Induced by a New Polysaccharide Isolated From Polyporus umbellatus (Pers.) Fries

Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model

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