Pine needle hexane extract promote cell cycle arrest and premature senescence via p27KIP1 upregulation gastric cancer cells

Abstract

Pinus densiflora sieb. et zucc.(pine needle) is a traditional medicine used in several East Asian countries. However, the efficacy of pine needle has rarely been reported. In this study showed that the anti-proliferative effects and the mechanisms of hexane layer of pine needle MeOH extract (PNH) on gastric cancer cells. At first, PNH inhibited the proliferation of gastric cancer cells in a dosedependent manner. Moreover, PNH treatment induced G1 phase cell cycle arrest through the increased p27 KIP1 expression and decreased cyclin dependent kinase (CDKs) activity. Furthermore, PNH treatment induced premature senescence without oncogenic stress, through the expression of p27 KIP1 and Skp2. Taken together, these results showed that PNH inhibited gastric cancer cell proliferation through the induction of G1-cell cycle arrest and premature senescence via induced p27 KIP1 expression, as controlled by Skp2 reduction. Also, PNH could be a candidate for anti-gastric cancer treatment and may be useful in the development of anti-gastric cancer drugs.

Therapeutic Potential of Antimicrobial Peptide PN5 against Multidrug-Resistant E. coli and Anti-Inflammatory Activity in a Septic Mouse Model

“…Needles, pollen, cones, and cortices derived from pine trees have been used as antibacterial, anti-inflammatory, and preservative agents in traditional medicine ( 29 ). Particularly, pine needles are crude drugs that are effective for preventing diseases such as rheumatism, gastroenteric trouble, and asthma ( 30 , 31 ). PN5, which consists of 11 amino acids, was isolated from pine needles and showed antibacterial activity against foodborne bacteria in a previous study ( 20 ).…”

A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria

“…Pine bark extracts are effective scavengers of free radicals and reactive oxygen species, can lower lipid levels in blood serum and may help to prevent disease and delay aging ( Schafer et al, 2006 ; McGrath et al, 2015 ). PNs of form P. densiflora were used for folk medicine and for various disease prevention such as rheumatitis, hemorrhage, gastroenteric trouble, hypertension, and asthma ( Kwak et al, 2006 ; Kim et al, 2020b ). Recent scientific researches have shown that PNs form P. densiflora have antimicrobial, anti-viral, antioxidant, anti-mutagenic, anti-thrombosis, anti-asthmatic, and anti-inflammatory, and anti-cancer effects ( Kwak et al, 2006 ; Park and Lee, 2011 ; Park et al, 2016 ; Ahn et al, 2018 ; Mostafa et al, 2018 ; Ha et al, 2020 ).…”