Pinacyanol Erythrosinate as A Stain for Mast Cells

Bensley, Sylvia H.

doi:10.3109/10520295209105090

Cited by 56 publications

(21 citation statements)

References 3 publications

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“…PE selectively stains and differentiates mast cells (5). The total mast cell population in the LV section was counted and divided by the tissue area obtained from a digitized image of the section to yield the number of mast cells per square millimeter.…”

Section: Mast Cell Densitymentioning

confidence: 99%

Estrogen attenuates chronic volume overload induced structural and functional remodeling in male rat hearts

Gardner

Murray

Voloshenyuk

et al. 2010

American Journal of Physiology-Heart and Circulatory Physiology

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We have previously reported gender differences in ventricular remodeling and development of heart failure using the aortocaval fistula model of chronic volume overload in rats. In contrast to males, female rats exhibited no adverse ventricular remodeling and less mortality in response to volume overload. This gender-specific cardioprotection was lost following ovariectomy and was partially restored using estrogen replacement. However, it is not known if estrogen treatment would be as effective in males. The purpose of this study was to evaluate the structural and functional effects of estrogen in male rats subjected to chronic volume overload. Four groups of male rats were studied at 3 days and 8 wk postsurgery as follows: fistula and sham-operated controls, with and without estrogen treatment. Biochemical and histological studies were performed at 3 days postsurgery, with chronic structural and functional effects studied at 8 wk. Measurement of systolic and diastolic pressure-volume relationships was obtained using a blood-perfused isolated heart preparation. Both fistula groups developed significant ventricular hypertrophy after 8 wk of volume overload. Untreated rats with fistula exhibited extensive ventricular dilatation, which was coupled with a loss of systolic function. Estrogen attenuated left ventricular dilatation and maintained function in treated rats. Estrogen treatment was also associated with a reduction in oxidative stress and circulating endothelin-1 levels, as well as prevention of matrix metalloproteinase-2 and -9 activation and breakdown of ventricular collagen in the early stage of remodeling. These data demonstrate that estrogen attenuates ventricular remodeling and disease progression in male rats subjected to chronic volume overload.

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Section: Mast Cell Densitymentioning

confidence: 99%

Estrogen attenuates chronic volume overload induced structural and functional remodeling in male rat hearts

Gardner

Murray

Voloshenyuk

et al. 2010

American Journal of Physiology-Heart and Circulatory Physiology

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“…Left ventricular tissues were fixed in 10 % buffered formalin and subsequently processed for routine histology, with paraffin embedded 5 µm cross-sections from each heart stained with the mast cell specific stain, pinacynol-erythrosynate [25]. The number of mast cells present on the epicardial surface of the ventricular cross-section were determined in hearts that had undergone the isolation procedure, as well as in control hearts that had not, in order to determine if the isolated population of mast cells originated from the epicardial surface of these hearts.…”

Section: Histology: Mast Cell Stainingmentioning

confidence: 99%

A novel technique for isolating functional mast cells from the heart

et al. 2008

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“…The apical portion was snap frozen in liquid nitrogen and stored at Ϫ80°C for subsequent use. Mast cell density was determined from 5-m-thick coronal sections that were stained with the mast cell-specific stain, pinacyanol erythrosinate, 13 with mast cell density determined by dividing the total number of mast cells by the area of the respective LV cross-section. Collagen volume fraction was determined as described previously, 14 with 5-m-thick paraffin-embedded sections stained with picrosirius red (0.1% Sirius Red F3BA in picric acid) after incubation in phosphomolybdic acid (0.2%).…”

Section: Histology: Cardiac Mast Cell Density and Collagen Volume Framentioning

confidence: 99%

Cardiac Mast Cells Mediate Left Ventricular Fibrosis in the Hypertensive Rat Heart

et al. 2009

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Abstract-Correlative data suggest that cardiac mast cells are a component of the inflammatory response that is important to hypertension-induced adverse myocardial remodeling. However, a causal relationship has not been established. We hypothesized that adverse myocardial remodeling would be inhibited by preventing the release of mast cell products that may interact with fibroblasts and other inflammatory cells. Eight-week-old male spontaneously hypertensive rats were treated for 12 weeks with the mast cell stabilizing compound nedocromil (30 mg/kg per day). Age-matched Wistar-Kyoto rats served as controls. Nedocromil prevented left ventricular fibrosis in the spontaneously hypertensive rat independent of hypertrophy and blood pressure, despite cardiac mast cell density being elevated. The mast cell protease tryptase was elevated in the spontaneously hypertensive rat myocardium and was normalized by nedocromil. Treatment of isolated adult spontaneously hypertensive rat cardiac fibroblasts with tryptase induced collagen synthesis and proliferation, suggesting this as a possible mechanism of mast cell-mediated fibrosis. In addition, nedocromil prevented macrophage infiltration into the ventricle. The inflammatory cytokines interferon-␥ and interleukin (IL)-4 were increased in the spontaneously hypertensive rat and normalized by nedocromil, whereas IL-6 and IL-10 were decreased in the spontaneously hypertensive rat, with nedocromil treatment normalizing IL-6 and increasing IL-10 above the control. These results demonstrate for the first time a causal relationship between mast cell activation and fibrosis in the hypertensive heart. Furthermore, these results identify several mechanisms, including tryptase, inflammatory cell recruitment, and cytokine regulation, by which mast cells may mediate hypertension-induced left ventricular fibrosis.

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Pinacyanol Erythrosinate as A Stain for Mast Cells

Cited by 56 publications

References 3 publications

Estrogen attenuates chronic volume overload induced structural and functional remodeling in male rat hearts

Estrogen attenuates chronic volume overload induced structural and functional remodeling in male rat hearts

A novel technique for isolating functional mast cells from the heart

Cardiac Mast Cells Mediate Left Ventricular Fibrosis in the Hypertensive Rat Heart

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