Pin1At regulates PIN1 polar localization and root gravitropism

Xi, Wanyan; Gong, Xun; Yang, Qiaoyun; Yu, Hao; Liou, Yih‐Cherng

doi:10.1038/ncomms10430

Cited by 50 publications

(46 citation statements)

References 64 publications

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“…Interestingly, a large-scale sequence alignment of multiple PIN HL sequences from land plants reveals some HL conserved regions, many of which contain known phosphorylation sites (Bennett et al., 2014) regulating PIN function (Ganguly et al., 2012). These motifs have to be accessible to cytoplasmic enzymes that modify the phosphorylation status (Michniewicz et al., 2007, Zhang et al., 2010, Barbosa et al., 2014, Zourelidou et al., 2014) and conformation (Xi et al., 2016) of the HL, supporting its intracellular localization also in congruence with our findings for Arabidopsis PM PINs. This also illustrates the adequacy of choosing the HL as the first protein region to be topologically mapped, as its likely intracellular orientation was a good validation of our experimental methods.…”

Section: Discussionmentioning

confidence: 99%

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

et al. 2016

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Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques, we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five α-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1. Since the canonical long PINs show a high degree of conservation in TM domains and auxin transport capacity has been demonstrated for Arabidopsis representatives of this group, this empirically enhanced topological model of PIN1 will be an important starting point for further studies on PIN structure–function relationships. In addition, we have established protocols that can be used to probe the topology of other plasma membrane proteins in plants.

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Section: Discussionmentioning

confidence: 99%

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

et al. 2016

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“…SNP ( BOPA2_12_11044) at 86 cM on 7H associated with the observed variations in the number of nodal roots is physically located in the domain of PPIB (HORVU7Hr1G095720). PPIB is catalyzed by LATERAL ROOTLESS2 to regulate root gravitropism, lateral root development, and induce thermo‐tolerance (Xi et al, ; Kaur et al, ). Root gravitropism is a physiological drought response that redirects root growth by gravitational pull toward the down water sources via auxin transport.…”

Section: Discussionmentioning

confidence: 99%

Section: Candidate Genes In the Detected Qtl Regions For Root Watermentioning

confidence: 99%

Genetic components of root architecture and anatomy adjustments to water‐deficit stress in spring barley

Oyiga

Palczak

Wojciechowski

et al. 2019

Plant Cell & Environment

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Roots perform vital roles for adaptation and productivity under water‐deficit stress, even though their specific functions are poorly understood. In this study, the genetic control of the nodal‐root architectural and anatomical response to water deficit were investigated among diverse spring barley accessions. Water deficit induced substantial variations in the nodal root traits. The cortical, stele, and total root cross‐sectional areas of the main‐shoot nodal roots decreased under water deficit, but increased in the tiller nodal roots. Root xylem density and arrested nodal roots increased under water deficit, with the formation of root suberization/lignification and large cortical aerenchyma. Genome‐wide association study implicated 11 QTL intervals in the architectural and anatomical nodal root response to water deficit. Among them, three and four QTL intervals had strong effects across seasons and on both root architectural and anatomical traits, respectively. Genome‐wide epistasis analysis revealed 44 epistatically interacting SNP loci. Further analyses showed that these QTL intervals contain important candidate genes, including ZIFL2, MATE, and PPIB, whose functions are shown to be related to the root adaptive response to water deprivation in plants. These results give novel insight into the genetic architectures of barley nodal root response to soil water deficit stress in the fields, and thus offer useful resources for root‐targeted marker‐assisted selection.

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“…Surface sterilized (0.1 % Triton X in 95% ethanol) Arabidopsis thaliana wild type (Col-0 ecotype) seeds were sown on 1xMS-agar (Murashige and Skoog Basal Salt Mixture, Sigma M5524) (Xi et al 2016) plates (50 mL) containing 1% sucrose (w/v) and 0.7% (w/v) agar (Sigma A1296).The seeds were kept at 4°C in darkness for minimum of 2 days (stratification) and then transferred to a growth chamber set at 23°C in 16/8 h light and dark cycles (long day cycle, LD). Selenium and Bacterial suspensions of Pseudomonas sp.…”

Section: Methods Materialsmentioning

confidence: 99%

High-resolution synchrotron imaging studies of intact fresh roots reveal soil bacteria promoted bioremediation and bio-fortification

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Lusa

Honkanen

et al. 2019

Preprint

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Plant-microbe interactions can be utilized in bio-based processes such as bioremediation and biofortification, either to remove hazardous radionuclides and heavy metals from the soil, or to increase the accumulation of desired elements into crops to improve their quality. Optimizing such elegant plant-microbe interactions requires detailed understanding of the chemical element compositions of fresh plant tissues at cellular organelle resolution. However, such analyses remain challenging because conventional methods lack the required spatial resolution, contrast or sensitivity. Using a novel combination of nanoscaleresolution 3D cryogenic synchrotron-light ptychography, holotomography and fluorescence tomography, we show how soil bacteria interact with Arabidopsis thaliana and promote the uptake of various metals. Co-cultivation with Pseudomonas sp. strain T5-6-I alters root anatomy and increases levels of selenium (Se), iron (Fe) and other micronutrients in roots. Our approach highlights the interaction of plants and microbes in bioremediation and biofortification on the subcellular level.

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Pin1At regulates PIN1 polar localization and root gravitropism

Cited by 50 publications

References 64 publications

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

Genetic components of root architecture and anatomy adjustments to water‐deficit stress in spring barley

High-resolution synchrotron imaging studies of intact fresh roots reveal soil bacteria promoted bioremediation and bio-fortification

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