PIM2 promotes lung adenocarcinoma cell migration by regulating XIAP/NF-κB pathway

Abstract

Background and objective: Proviral insertion site in Moloney murine leukemia virus (PIM)2 functions as a serine/threonine kinase to participate in regulating cell proliferation and cell cycle. PIM2 has been shown to be elevated in the lung cancer cell lines. This study was performed to investigate the role of PIM2 in lung adenocarcinoma cell growth. Mateial and methods: Expression level of PIM2 in lung adenocarcinoma tissues and cells was detected by qRT-PCR (quantitative Reverse Transcription PCR) and western blot. The over-expression and knockdown of PIM2 were separately established by employing pcDNA and siRNA to explore the effects on the cell viability, apoptosis, invasion and migration. The downstream pathways were evaluated by western blot assay. Results: Lung adenocarcinoma tissues and cells showed an elevation of both PIM2 mRNA and protein expression. Knocking down PIM2 decreased the cell viability and promoted the apoptosis, which can be reversed by pcDNA-mediated over-expression of PIM2. PIM2 silencing suppressed the promotional effect of over-expression of PIM2 on cell invasion and migration through increasing IκBα expression and decreasing the X-linked inhibitor of apoptosis protein (XIAP), p65 and IκBα phosphorylation. While, over-expression of PIM2 showed opposite effect on IκBα and XIAP expression or p65 and IκBα phosphorylation. Conclusion: PIM2 can not only suppress lung adenocarcinoma cell apoptosis but also promote cell migration and invasion depending on XIAP/NF-κB signaling pathway.

CTRP3 regulates NF-κB and TGFβ1/Smad3 pathways to alleviate airway inflammation and remodeling in asthmatic mice induced by OVA

“…These results were comparable with the results of Pu et al 37 NF-κB is a potent inflammatory mediator that enhances cytokine production in a variety of diseases, including asthma. 38,39 Phosphorylation of IκB activates the NF-κB p65 subunit, which is responsible for increasing cytokine production and promoting inflammatory cascade reaction. 38,40 TGF-β1 is a multipotent cytokine involved in airway inflammation and fibrotic tissue remodeling during asthma pathology.…”

“…38,39 Phosphorylation of IκB activates the NF-κB p65 subunit, which is responsible for increasing cytokine production and promoting inflammatory cascade reaction. 38,40 TGF-β1 is a multipotent cytokine involved in airway inflammation and fibrotic tissue remodeling during asthma pathology. TGF-β1 functions through a number of different signaling pathways, including the Smad pathway.…”