Pilot Testing of Pichia guilliermondii: A Biocontrol Agent of Postharvest Diseases of Citrus Fruit

Droby, Samir; Hofstein, R.; Wilson, Charles L.; Wisniewski, Michael; Fridlender, Bertold; Cohen, Lea; Weiss, Batia; Daus, Avinoam; Timar, D.; Chalutz, E.

doi:10.1006/bcon.1993.1008

Cited by 102 publications

(38 citation statements)

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“…This yeast species has been widely reported as an effective BCA against fungal pathogens, including blue and green mould of citrus fruits (Droby et al, 1993;Kinay and Yildiz, 2008), tomato spoilages (Saligkarias et al, 2002;Zhao et al, 2010) and Colletotrichum capsici on chilli fruit (Nantawanit et al, 2010). In all cases, the efficacy of an antagonistic yeast was dependent on the antagonist concentration and the pathogen inoculum concentration.…”

Section: Discussionmentioning

confidence: 99%

Efficacy assessment of Pichia guilliermondii strain Z1, a new biocontrol agent, against citrus blue mould in Morocco under the influence of temperature and relative humidity

et al. 2011

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a b s t r a c tThe interactions of Penicillium italicum, which causes blue mould, and antagonistic yeast Pichia guilliermondii strain Z1 were examined in controlled environments, to determine the influence of relative humidity (RH) (45%, 75%, 85%, 98%, and 100%) and temperature (T) (5, 10, 15, 20, and 25°C). All main effects and interactions were significant (P 6 0.05), with the exception of interactions RHÂT and strain Z1 (BCA)ÂRHÂT. In the pathogen control, the lesion diameter of blue mould developed under all environmental conditions but was the largest at a RH range between 98% and 100%, independent of the temperature. The efficacy of strain Z1 appeared to be independent of the environment and reduced disease incidence by more than 85% in all environmental conditions. Rapid colonization of the antagonistic yeast strain Z1 on citrus wounded sites was recorded during the first week at 5°C. Colonization then stabilized at ±6.9 Â 10 6 CFU/ml for 30 days. This indicates that P. guilliermondii is able to adapt itself and colonize the wound sites prior to the arrival of the pathogen, displaying greater efficiency than when colonizing wounds after pathogen. The antagonist was capable of growing in low concentrations of orange juice (0.1-5%), with greatest growth at 5%. Applying strain Z1 (1 Â 10 8 CFU/ml) as a formulated product significantly reduced the incidence of infected fruits and the percentage of infected wounds relative to the pathogen control. Disease control with formulated product (45%) was slightly lower than that obtained with thiabendazole (20%) or strain Z1 culturable cells (25%). These results suggest that strain Z1 may be a useful BCA for control of blue mould under varying environmental conditions, and control may be enhanced by combining with other eco-friendly post-harvest treatments or improved formulation.

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Section: Discussionmentioning

confidence: 99%

Efficacy assessment of Pichia guilliermondii strain Z1, a new biocontrol agent, against citrus blue mould in Morocco under the influence of temperature and relative humidity

et al. 2011

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“…Also, calcium has been considered to increase biocontrol efficacy of antagonists. It may also replace the current requirement for addition of low concentrations of fungicides to ensure consistent performance of yeast control agents under large-scale and commercial conditions (Droby et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Salts Application for Suppressing Potato Early Blight Disease

El-Mougy¹,

Abdel-Kader²

2009

Journal of Plant Protection Research

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Abstract:The suppressive effect of sodium and calcium salts applied individually or combined with the yeast Saccharomyces cerevisiae against Alternaria solani the causal agent of early blight disease of potato was evaluated under laboratory, greenhouse and field conditions. In vitro test a complete inhibition in fungal growth was observed at concentration of 30 mg/ml of both sodium bicarbonate and calcium chloride. The commercial backing yeast S. cerevisiae (CBY) enhanced the inhibitory effect of tested salts reflected in increasing mycelial fungal growth reduction when combined at the rate of 1:1 at each concentration tested. In pot experiment, under artificial infestation with pathogenic fungus, application of sodium bicarbonate or calcium chloride significantly reduced the early blight incidence and severity by increasing their concentrations. Their most effective concentration were 30 mg/ml that reduced the disease incidence by 50 and 62.4%, respectively. Superior effect of sodium bicarbonate or calcium chloride in disease reduction was observed when they combined with CBY. Field trails for evaluating the most promising greenhouse treatments were preformed under natural infestations during two successive summer seasons. Calcium chloride proved higher efficacy for reducing both disease incidence and severity than that of sodium bicarbonate when applied either alone or combined with CBY. Also, it is observed that increasing concentrations of both sodium bicarbonate or calcium chloride showed parallel decrease in disease incidence and severity. Application of (CBY) enhanced the efficacy of salts spraying against early blight disease. Similar trend was also observed with the increase of potato tubers yield. On the light of the present study it could be suggested that the usage of combined application of the yeast S. cerevisiae with sodium bicarbonate or calcium chloride might be used as easily applied, safely and cost effective control methods against such plant diseases.

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“…Tie compatibiliry of a strain o1 P. guilliermondii witha commercial wax and a fungicide (thiabendazot. ; *u, evaluated against P' digitatum on postharvest citrus (Droby et al, 1993). Oitrer investigators observed-the same phenomenon with other biochemical treatments (McGuire, 1994;Chand-Goyal and Spotts, 1996)' In some cases, the combination of biological uni ,h.rnirui applications allowed the reduction of chemical concentration (Droby et al ,1993) Sporesorhyphaelocatedonskinandonexternalpartoffruitflesh(Bondoux,1992;Eckert, 1975).…”

Section: Tntegration Of Biological Control To Other Treatments Againsmentioning

confidence: 70%

“…; *u, evaluated against P' digitatum on postharvest citrus (Droby et al, 1993). Oitrer investigators observed-the same phenomenon with other biochemical treatments (McGuire, 1994;Chand-Goyal and Spotts, 1996)' In some cases, the combination of biological uni ,h.rnirui applications allowed the reduction of chemical concentration (Droby et al ,1993) Sporesorhyphaelocatedonskinandonexternalpartoffruitflesh(Bondoux,1992;Eckert, 1975). However,thermotherapytreatmentwithrvaterbathcanenhancethesensitivityof applestowoundpathogens,u.r,u,Alternariasp',Bcitrcreoorf''ex(lll'\llfi(Edneyand Burchill, 1967).…”

Section: Tntegration Of Biological Control To Other Treatments Againsmentioning

confidence: 74%

“…In this respect' an elegant and fast method of antagonist isolation has been adopted by Wilson et al, (1993) They applied rinsing waters from tomatoes and apples directly on wounds inoculated with the pattiogàn (8. cinerea) and isolated antagonistic micro-organisms from wounds which did not exhibit any symptom. We isolated mic5p-organisms from rinsing waters of Golden Delicious at harvest and after different periods of'storage (Jrjakli, 1996).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Yeast Species for Biocontrol of Apple Postharvest Diseases: An Encouraging Case of Study for Practical Use

Jijakli

Lepoivre

Grevesse

1999

Biotechnological Approaches in Biocontrol of Plant Pathogens

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Pilot Testing of Pichia guilliermondii: A Biocontrol Agent of Postharvest Diseases of Citrus Fruit

Cited by 102 publications

References 0 publications

Efficacy assessment of Pichia guilliermondii strain Z1, a new biocontrol agent, against citrus blue mould in Morocco under the influence of temperature and relative humidity

Efficacy assessment of Pichia guilliermondii strain Z1, a new biocontrol agent, against citrus blue mould in Morocco under the influence of temperature and relative humidity

Salts Application for Suppressing Potato Early Blight Disease

Yeast Species for Biocontrol of Apple Postharvest Diseases: An Encouraging Case of Study for Practical Use

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