Pilot study: bone marrow stem cells as a treatment for dogs with chronic spinal cord injury

Sarmento, Carlos Alberto Palmeira; Rodrigues, Márcio Nogueira; Bocabello, Renato Zonzini; Mess, Andrea; Miglino, María Angélica

doi:10.1186/2050-490x-2-9

Cited by 34 publications

(35 citation statements)

References 29 publications

(31 reference statements)

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“…This study is the first to provide objective evidence of the feasibility and therapeutic potential of intraspinal (nonsurgical) OEC transplantation in chronic spinal cord injury (SCI). Along with the study of allogeneic fetal canine BM‐MSCs in IVDD and NLBM‐MSC , this study demonstrates that use of percutaneous delivery represents a viable nonsurgical route of administration that has not been fully appreciated in past studies. Questions remain concerning the method of isolation and characterization of OEC, given the challenge to access lamina propria tissue from olfactory mucosa; biopsy of olfactory bulb has been proposed as an alternative source of OEC (in humans) .…”

Section: Study Disease Targetsmentioning

confidence: 87%

Concise Review: Stem Cell Trials Using Companion Animal Disease Models

2016

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Studies to evaluate the therapeutic potential of stem cells in humans would benefit from more realistic animal models. In veterinary medicine, companion animals naturally develop many diseases that resemble human conditions, therefore, representing a novel source of preclinical models. To understand how companion animal disease models are being studied for this purpose, we reviewed the literature between 2008 and 2015 for reports on stem cell therapies in dogs and cats, excluding laboratory animals, induced disease models, cancer, and case reports. Disease models included osteoarthritis, intervertebral disc degeneration, dilated cardiomyopathy, inflammatory bowel diseases, Crohn's fistulas, meningoencephalomyelitis (multiple sclerosis-like), keratoconjunctivitis sicca (Sjogren's syndrome-like), atopic dermatitis, and chronic (end-stage) kidney disease. Stem cells evaluated in these studies included mesenchymal stemstromal cells (MSC, 17/19 trials), olfactory ensheathing cells (OEC, 1 trial), or neural lineage cells derived from bone marrow MSC (1 trial), and 16/19 studies were performed in dogs. The MSC studies (13/17) used adipose tissue-derived MSC from either allogeneic (8/13) or autologous (5/13) sources. The majority of studies were open label, uncontrolled studies. Endpoints and protocols were feasible, and the stem cell therapies were reportedly safe and elicited beneficial patient responses in all but two of the trials. In conclusion, companion animals with naturally occurring diseases analogous to human conditions can be recruited into clinical trials and provide realistic insight into feasibility, safety, and biologic activity of novel stem cell therapies. However, improvements in the rigor of manufacturing, study design, and regulatory compliance will be needed to better utilize these models. STEM CELLS 2016;34:1709-1729 SIGNIFICANCE STATEMENTStudies in veterinary medicine which have employed companion animals to evaluate safety and efficacy of stem cells have not been systematically reviewed for the human medical and biomedical research community. The goal of this review is to shed light on examples whereby companion animal spontaneous disease models (i.e., veterinary patients) were utilized to study novel stem cell therapies, and to stimulate further discussion on the potential value of these models in multidisciplinary studies for the dual benefit of human and veterinary medicine.

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Section: Study Disease Targetsmentioning

confidence: 87%

Concise Review: Stem Cell Trials Using Companion Animal Disease Models

2016

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“…Apart from preclinical models, dMSCs have been applied in canine clinical spinal cord injuries (Bhat et al, 2019;Escalhão et al, 2017;Granger et al, 2012;Kim et al, 2011;J. H. Lee, Chung, et al, 2011;Nishida et al, 2011;Penha et al, 2014;Sarmento et al, 2014) (Bhat et al, 2019). Besides clinical scores, significantly better histological scores (Lim et al, 2007;Ryu et al, 2009) and lower fibrosis (S. H. Park et al, 2011;Park et al, 2012b), and fewer cavitations were seen on MRI (Jung et al, 2009;, J. H. Lee, Chung, et al, 2011Lim et al, 2007) in cell treated groups compared with control/sham groups.…”

Section: Nervous Systemmentioning

confidence: 99%

Mesenchymal stem cell basic research and applications in dog medicine

Gugjoo

Amarpal

Sharma

2019

Journal Cellular Physiology

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The stem cells, owing to their special characteristics like self‐renewal, multiplication, homing, immunomodulation, anti‐inflammatory, and dedifferentiation are considered to carry an “all‐in‐one‐solution” for diverse clinical problems. However, the limited understanding of cellular physiology currently limits their definitive therapeutic use. Among various stem cell types, currently mesenchymal stem cells are extensively studied for dog clinical applications owing to their readily available sources, easy harvesting, and ability to differentiate both into mesodermal, as well as extramesodermal tissues. The isolated, culture expanded, and characterized cells have been applied both at preclinical as well as clinical settings in dogs with variable but mostly positive results. The results, though positive, are currently inconclusive and demands further intensive research on the properties and their dependence on the applications. Further, numerous clinical conditions of dog resemble to that of human counterparts and thus, if proved rewarding in the former may act as basis of therapy for the latter. The current review throws some light on dog mesenchymal stem cell properties and their potential therapeutic applications.

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“…In the study of SCI, several species of experimental animals, for example, pigs (Jones et al, ; Lee et al, ; Šulla et al, ), dogs (Fujimaki et al, ; Bozynski et al, ; Sarmento et al, ), cats (Tsitsopoulos et al, ; Gu et al, ; Murphy et al, ), rabbits (Fang et al, ; Leonard et al, ; Mechírová et al, ), guinea pigs (Ouyang et al, ; Galle et al, ; Sun et al, ), rats (Chung et al, ; Losey and Anthony, ; Soubeyrand et al, ), and mice (Abematsu et al, ; Awad et al, ; Gaviria et al, 2012) are used.…”

mentioning

confidence: 99%

Arterial Blood Supply to the Spinal Cord in Animal Models of Spinal Cord Injury. A Review

Mazensky

Flešárová

Šulla

2017

The Anatomical Record

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Animal models are used to examine the results of experimental spinal cord injury. Alterations in spinal cord blood supply caused by complex spinal cord injuries contribute significantly to the diversity and severity of the spinal cord damage, particularly ischemic changes. However, the literature has not completely clarified our knowledge of anatomy of the complex three-dimensional arterial system of the spinal cord in experimental animals, which can impede the translation of experimental results to human clinical applications. As the literary sources dealing with the spinal cord arterial blood supply in experimental animals are limited and scattered, the authors performed a review of the anatomy of the arterial blood supply to the spinal cord in several experimental animals, including pigs, dogs, cats, rabbits, guinea pigs, rats, and mice and created a coherent format discussing the interspecies differences. This provides researchers with a valuable tool for the selection of the most suitable animal model for their experiments in the study of spinal cord ischemia and provides clinicians with a basis for the appropriate translation of research work to their clinical applications. Anat Rec, 300:2091-2106, 2017. © 2017 Wiley Periodicals, Inc.

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Pilot study: bone marrow stem cells as a treatment for dogs with chronic spinal cord injury

Cited by 34 publications

References 29 publications

Concise Review: Stem Cell Trials Using Companion Animal Disease Models

Concise Review: Stem Cell Trials Using Companion Animal Disease Models

Mesenchymal stem cell basic research and applications in dog medicine

Arterial Blood Supply to the Spinal Cord in Animal Models of Spinal Cord Injury. A Review

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