Pilot study and bioinformatics analysis of differentially expressed genes in adipose tissues of rats with excess dietary intake

Abstract

excessive adipose tissue accumulation is an increasing health problem worldwide. The present study aimed to determine differentially expressed genes (deGs) that are associated with the excessive accumulation of adipose tissues by Pcr arrays in an excess dietary intake animal model. For this purpose, male Sprague dawley rats were randomly assigned to 2 groups: control (given an ordinary diet) and experimental (given twice the amount of the ordinary diet). after 2 months of feeding, the abdominal cavities of the rats from each group were opened, then subcutaneous and visceral adipose tissues were removed. The adipose tissues collected were then used for total rna extraction and then reverse transcribed to cdna, which was then used as a template to identify the deGs of 84 transcripts for rat obesity by rT 2 Profiler PCR Arrays. The results showed significant downregulation of bombesin-like receptor 3 (BrS3) and uncoupling protein 1 (ucP1) in visceral adipose tissues of experimental rats compared with those of the control rats, and differential gene expression analysis showed an association with fat cell differentiation and regulation of triglyceride sequestration, as well as fatty acid binding. The gene expression patterns observed in the present study, which may be associated with peroxisome proliferator-activated receptor-γ (PParG) on excessive visceral adipose tissue accumulation, may be useful in identifying a group of surrogate biomarkers for the early diet-induced accumulation of visceral adipose tissue detection in humans. The biomarkers can also be the specific targets for drug development to reduce excessive visceral adipose tissue accumulation in the body and its associated diseases.

Inflammatory landscape in Xeroderma pigmentosum patients with cutaneous melanoma

“…Although this work is done on a limited number of samples, it is the first study that has focused on the immune landscape in cutaneous melanoma in patients with DNA repair disorders as XP. It is likely that a small 88 . However, the current research identified the same patterns of gene expression as shown in previous studies that investigated melanoma cancer such as IL8 and TGFB 89 as well as in the RNA-seq databases that we investigated in this work.…”